We narrowed to 11,083 results for: HA

1. pCMV-T7-SpCas9(MKKCMN)-P2A-EGFP (AHK129)

   Plasmid

   #223077

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKKCMN amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(MKKCMN)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(MKKCMN)=D1135M, S1136K, G1218K, E1219C, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-T7-SpCas9(KKKCMN)-P2A-EGFP (AHK131)

   Plasmid

   #223078

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KKKCMN amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(KKKCMN)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(KKKCMN)=D1135K, S1136K, G1218K, E1219C, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-T7-SpCas9(MRRWMR)-P2A-EGFP (RAS2816)

   Plasmid

   #223068

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(MRRWMR)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-T7-SpCas9(ARGIMR)-P2A-EGFP (AHK147)

   Plasmid

   #223069

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(ARGIMR)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(ARGIMR)=D1135A, S1136R, G1218G, E1219I, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-T7-SpCas9(ARTWGR)-P2A-EGFP (RAS2995)

   Plasmid

   #223070

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with ARTWGR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(ARTWGR)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(ARTWGR)=D1135A, S1136R, G1218T, E1219W, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PB-Neo-TetO-Cdk13_Nterm_FlagHa

   Plasmid

   #127345

   Purpose

   N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vector

   Depositor

   Phil Sharp

   Article

   Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

   Insert

   Cyclin Dependent Kinase 13 (Cdk13 Mouse)
   

   Use

   Piggybac transposase vector

   Tags

   Flag- HA- Tandem Epitopes

   Expression

   Mammalian

   Mutation

   Base pairs 1-1554 of transgene are codon optimize…

   Promoter

   TetO Promoter

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV.hSynap.iAChSnFR-Venus-NULL

   Plasmid

   #137953

   Purpose

   Expresses iAChSnFR (non-binding yellow version) under hSynapsin promoter

   Depositor

   Loren Looger

   Article

   Borden et al bioRxiv 2020

   Insert

   iAChSnFR (yellow version, non-binding variant)
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   cpSFGFP replaced with cpSFVenus; Y140A binding si…

   Promoter

   hSynapsin

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR223_HRAS_p.A11D

   Plasmid

   #81305

   Purpose

   Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   HRAS (HRAS Human)
   

   Use

   Gateway entry vector

   Mutation

   A11D

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR223_HRAS_p.A59T

   Plasmid

   #81292

   Purpose

   Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   HRAS (HRAS Human)
   

   Use

   Gateway entry vector

   Mutation

   A59T

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV.hSynapsin.SF-iGluSnFR.S72A (AAV1)

    Viral Prep

    #106176-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.hSynapsin.SF-iGluSnFR.S72A (#106176). In addition to the viral particles, you will also receive purified pAAV.hSynapsin.SF-iGluSnFR.S72A plasmid DNA. hSynapsin driven SF-iGluSnFR.S72A, glutamate sensor. The S72A mutant has lower affinity for glutamate. Slower on-rate and faster off-rate. Enhanced temporal resolution and spatial resolution. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Promoter

    Syn

    Available Since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

11. eScaf

    Bacterial Strain

    #220921

    Purpose

    cssDNA production strain

    Depositor

    Shawn Douglas

    Article

    Shen et al Nucleic Acids Res. 2024 Apr 24;52(7):409

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

12. BL21(DE3)-R3-pRARE2

    Bacterial Strain

    #26242

    Purpose

    Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Bacterial Resistance

    Chloramphenicol

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

13. Golden GATEway Cloning Kit

    Plasmid Kit

    #1000000054

    Purpose

    Combinatorial assembly method that combines Golden Gate (Type IIs endonucleases) cloning with Multisite Gateway (recombination) cloning to construct complex plasmids in an efficient and modular way

    Depositor

    Joachim Wittbrodt

    Available Since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

14. US0 pyrF-/hisB-/tnaA-

    Bacterial Strain

    #228512

    Purpose

    Indole-responsive Growth Selection

    Depositor

    Timothy Whitehead

    Article

    Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

    Bacterial Resistance

    Tetracycline

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

15. pMS2-GFP

    Plasmid

    #27121

    Depositor

    Robert Singer

    Article

    Fusco et al Curr Biol. 2003 Jan 21. 13(2):161-7.

    Insert

    MS2
    

    Tags

    EGFP, HA, and SV40 NLS

    Expression

    Mammalian

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-EF1a-DIO-hM4D(Gi)-mCherry

    Plasmid

    #50461

    Purpose

    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter

    Depositor

    Bryan Roth

    Insert

    hM4D(Gi)-mCherry (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA and mCherry

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    EF1a

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-P-RAN-H2A2B

    Plasmid

    #106412

    Purpose

    P-RAN-H2A2B (RANbody H2A2B::vHRP)

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

    Insert

    P-RAN-H2A2B
    

    Tags

    HA and His

    Expression

    Mammalian

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SnailHA_pcDNA3

    Plasmid

    #31697

    Depositor

    Paul Wade

    Article

    Kajita et al Mol Cell Biol. 2004 Sep . 24(17):7559-66

    Insert

    Snail (SNAI1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EBV BGLF4 KD

    Plasmid

    #26692

    Depositor

    Robert Kalejta

    Article

    Kuny et al PLoS Pathog. 2010 . 6(9):.

    Insert

    BGLF4
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Kinase deficient mutant: Lysine 102 mutated to I…

    Available Since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

20. PKC epsilon DN

    Plasmid

    #21243

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC epsilon (Prkce Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K437R

    Available Since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only