We narrowed to 4,340 results for: Pet2;

1. pJK314

   Plasmid

   #71140

   Purpose

   Expression of Acetobacter aceti 1023 PurE (H89V mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   changes histidine-89 to valine; additional N-term…

   Promoter

   T7

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK174

   Plasmid

   #71835

   Purpose

   Expression of Acetobacter aceti 1023 PurE and PurK in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Expression

   Bacterial

   Mutation

   additional N-terminal methionine encoded by this …

   Promoter

   T7 and endogenous

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK279

   Plasmid

   #71135

   Purpose

   Expression of Acetobacter aceti 1023 PurE (H89D mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   changes histidine-89 to aspartate; additional N-t…

   Promoter

   T7

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK283

   Plasmid

   #71139

   Purpose

   Expression of Acetobacter aceti 1023 PurE (H59N mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   changes histidine-59 to asparagine; additional N-…

   Promoter

   T7

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK282

   Plasmid

   #71138

   Purpose

   Expression of Acetobacter aceti 1023 PurE (H59D mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   changes histidine-59 to aspartate; additional N-t…

   Promoter

   T7

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK281

   Plasmid

   #71137

   Purpose

   Expression of Acetobacter aceti 1023 PurE (H59F mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   changes histidine-59 to phenylalanine; additional…

   Promoter

   T7

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK280

   Plasmid

   #71136

   Purpose

   Expression of Acetobacter aceti 1023 PurE (H89F mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   changes histidine-89 to phenylalanine; additional…

   Promoter

   T7

   Available Since

   Jan. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK175

   Plasmid

   #71132

   Purpose

   Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   additional N-terminal methionine encoded by this …

   Promoter

   T7

   Available Since

   Jan. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pfMDH_Triple

   Plasmid

   #67733

   Purpose

   Expresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed histidine 303 to alanine, arginine 373 to…

   Promoter

   T7

   Available Since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pET:CACT:GFP

    Plasmid

    #60737

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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