We narrowed to 32,275 results for: uros

1. AAV-Syn-TRPV1-P2A-DsRed

   Plasmid

   #200830

   Purpose

   Transduce all neuron types with AAV to express TRPV1 along with a DsRed fluorescence reporter

   Depositor

   Hong Chen

   Article

   Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

   Insert

   TRPV1-P2A-DsRed (Trpv1 Rat)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Syn

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. mDlx-BiPOLES-mCerulean

   Plasmid

   #154946

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Insert

   BiPOLES
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   minimal Dlx

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5 FRT TO Myc ArA K162R

   Plasmid

   #59805

   Purpose

   Allows the integration of Myc ArA K162R in the genome and Tet-inducible expression

   Depositor

   Stephen Taylor

   Article

   Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

   Insert

   Aurora A (AURKA Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   K162R, kinase mutant

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV2-SynTetOff-FGL

   Plasmid

   #190234

   Purpose

   AAV vector, high-level expression of myrGFP with a somatodendritic-targeting signal (FGL) in neuronal cells

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Insert

   EGFP
   

   Use

   AAV

   Tags

   LDLR C-terminal sequence and myristoylation signa…

   Expression

   Mammalian

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. plenti hSyn GAPDH

   Plasmid

   #220918

   Purpose

   Neuronal specific expression of GAPDH

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   GAPDH (GAPDH Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Human a3 nicotinic receptor subunit EM construct

   Plasmid

   #163960

   Purpose

   pEZT-BM vector with alpha3 gene after CMV promoter, published EM construct

   Depositor

   Ryan Hibbs

   Article

   Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do

   Insert

   Human alpha3 nicotinic acetylcholine receptor subunit EM construct
   

   Use

   Baculovirus (bacmam) production and mammalian exp…

   Tags

   apocytochrome b(562)RIL (BRIL)

   Expression

   Mammalian

   Mutation

   Asn348-Ser402 replaced with apocytochrome b(562)R…

   Promoter

   CMV

   Available Since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Syn1-DIO-VGLUT2:miniSOG

   Plasmid

   #214200

   Purpose

   For Cre-dependent expression of VGLUT2:miniSOG (miniSOG is lumen-facing and HA-tagged)

   Depositor

   Thomas Hnasko

   Article

   Steinkellner et al ACS Chem Neurosci. 2021 Feb 17;12(4):626

   Insert

   Slc17a6 tagged with miniSOG and hemagglutinin
   

   Use

   AAV and Cre/Lox

   Tags

   miniSOG (tagged with HA) is fused to the first in…

   Expression

   Mammalian

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2xFLAGhYAP1-WW mutant

   Plasmid

   #17792

   Depositor

   Marius Sudol

   Article

   Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   Tryptophan 199 to Alanine; Proline 202 to Alanine

   Available Since

   May 9, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-GFP-IRES-mSNCA

   Plasmid

   #185714

   Purpose

   AAV expression of GFP and mouse α-Synuclein from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (Snca Mouse)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-ASAP2s

    Plasmid

    #101276

    Purpose

    Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

    Depositor

    Francois St-Pierre

    Article

    Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

    Insert

    ASAP2s
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn (human synapsin)

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pL-CRISPR.SFFV.PAC

    Plasmid

    #57829

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-CMV-cGAS C396/7A-HA

    Plasmid

    #130917

    Purpose

    Expresses human cGAS-HA with C396/397A point mutations; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    cGAS C396/7A
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    mutated residues 396 and 397 to alanines

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. hSyn-Aion-citrine

    Plasmid

    #192569

    Purpose

    AAV-vector for optogenetic long-term silencing of neurons

    Depositor

    Simon Wiegert

    Article

    Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

    Insert

    Aion
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    human synapsin

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc-y1sgRNA-Cas9

    Plasmid

    #49331

    Purpose

    Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

    Depositor

    Ji-Long Liu

    Article

    Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

    Inserts

    Cas9
    dU6-sgRNA
    y1 sgRNA (y Fly)
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Insect

    Mutation

    Human codon optimised

    Promoter

    Actin-5c and Drosophila U6

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. T7-VEE-CFP

    Plasmid

    #193357

    Purpose

    Self-replicating RNA vector expressing CFP fluorescent protein + PuroR

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    CFP
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hNR2F2-RFP

    Plasmid

    #22926

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    human nuclear receptor subfamily 2 group F member 2 promoter driving DsRedExpress (NR2F2 Human)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVS1-PdTK-CAG-mCh-[uBglII]

    Plasmid

    #107280

    Purpose

    puro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherry

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    AAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )
    

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-CMV-GCaMP3-373

    Plasmid

    #91779

    Purpose

    Mammalian expression of low affinity calcium-sensing fluorescent protein

    Depositor

    Douglas Kim, GENIE Project

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    GCaMP3-373
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP18 (CK2alpha' K69M)

    Plasmid

    #27090

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2alpha' K69M (CSNK2A2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K69M

    Promoter

    CMV

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pLCKO_LacZ_sgRNA

    Plasmid

    #74179

    Purpose

    lentiviral vector expressing sgRNA targeting LacZ

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    LacZ sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 Promoter

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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