We narrowed to 13,681 results for: crispr cas9

1. pMLS360

   Plasmid

   #73687

   Purpose

   Punc-47::2xNLS-FLP-D5 Expression vector

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Punc-47::2xNLS-FLP-D5::let-858 3'-UTR
   

   Use

   Flp/frt

   Expression

   Worm

   Promoter

   Punc-47

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB8383

   Plasmid

   #126917

   Purpose

   Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of tHMG1

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   tHMG1
   

   Expression

   Yeast

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-SpG-P2A-mScarlet (MNW006)

   Plasmid

   #174136

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDD375

   Plasmid

   #91825

   Purpose

   Codon-optimized mKate2 donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   mKate2-C1
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAP635-2

   Plasmid

   #99485

   Purpose

   Myc::TEV::3XFlag::Dendra2::3XFlag::TEV::Myc

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   Myc::TEV::3XFlag::Dendra2::3XFlag::TEV::Myc
   

   Expression

   Bacterial

   Available Since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDD397

   Plasmid

   #91822

   Purpose

   AID FP-slot donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Auxin-inducible degron (AID)
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. YN2_1_LT84_RPL13A

   Plasmid

   #177712

   Purpose

   Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDD398

   Plasmid

   #91832

   Purpose

   AID NT-tag linker donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Auxin-inducible degron (AID)
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAP685-2

   Plasmid

   #99490

   Purpose

   TEV::eGFP::ER::6xHis::linker::3XFlag

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   TEV::eGFP::RE::6Xhis::linker::3XFlag
   

   Expression

   Bacterial

   Available Since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. AByF

    Plasmid

    #111084

    Purpose

    Integrates marker gene onto D. melanogaster Y chromosome

    Depositor

    Omar Akbari

    Article

    Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

    Inserts

    Y homology arm left
    gypsy insulator
    3xP3 promoter
    tdTomato
    SV40 3'UTR
    CTCF insulator
    Y homology arm right
    

    Expression

    Insect

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB8382

    Plasmid

    #126916

    Purpose

    Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI
    

    Expression

    Yeast

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTLR-donor-UAS

    Plasmid

    #119001

    Purpose

    Repair template for the TLR reporter with 10 UAS binding sites for yeast Gal4

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    mVenus
    

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTLR-donor-tetO

    Plasmid

    #119000

    Purpose

    Repair template for the TLR reporter with 7 tet operator elements

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    mVenus
    

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAP639-2F

    Plasmid

    #99486

    Purpose

    3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag
    

    Expression

    Bacterial

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAP683-2

    Plasmid

    #99488

    Purpose

    TEV::eGFP::ER::HA::linker::3XFlag

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    TEV::eGFP::RE::HA::linker::3XFlag
    

    Expression

    Bacterial

    Available Since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAP607-2

    Plasmid

    #99483

    Purpose

    Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc
    

    Expression

    Bacterial

    Available Since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAP684-3

    Plasmid

    #99489

    Purpose

    TEV::eGFP::ER::Ollas::linker::3XFlag

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    TEV::eGFP::RE::Ollas::linker::3XFlag
    

    Expression

    Bacterial

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBHM2674

    Plasmid

    #241727

    Purpose

    Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains BSR marker for selection on blasticidin S

    Depositor

    Hiten Madhani

    Article

    Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Sept. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBHM2672

    Plasmid

    #241719

    Purpose

    Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains BLE marker for selection on phleomycin

    Depositor

    Hiten Madhani

    Article

    Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Sept. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC57-ITR2-H1-sgCTG-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222857

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(CTG)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgCTG
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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