We narrowed to 32,277 results for: uros

1. pGM32DEST

   Plasmid

   #74747

   Purpose

   Encodes for the QF-GR recombinant gene, splice linker, and mCherry reporter gene

   Depositor

   Jordan Ward, Keith Yamamoto

   Article

   Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

   Inserts

   Gateway cassette
   QF-GR (NR3C1 Synthetic, Human, Neurospora crassa)
   

   Tags

   Fusion of the Neurospora crassa QF activator to t…

   Expression

   Worm

   Promoter

   No promoter

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGP-CMV-GCaMP6-210

   Plasmid

   #91778

   Purpose

   Mammalian expression of low affinity calcium-sensing fluorescent protein

   Depositor

   Douglas Kim, GENIE Project

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   GCaMP6-210
   

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pXL003-ishRNA-beta-catenin-2

   Plasmid

   #36299

   Depositor

   Sean Palecek

   Article

   Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

   Insert

   shRNA of beta-catenin-2 (CTNNB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   IRES-RFP-Puro

   Expression

   Mammalian

   Promoter

   TetO-H1

   Available Since

   Aug. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

4. p323.hSyn.Halo-mFMRP.Wpre

   Plasmid

   #185909

   Purpose

   Expresses FMRP fused to HaloTag for protein localization through fluorescence imaging

   Depositor

   Young J Yoon

   Article

   Monday et al Neuron. 2022 Aug 17;110(16):2588-2606.e6

   Insert

   HaloTag fused to mouse Fragile X mental retardation protein (Ctsa Mouse)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Promoter

   hSyn (human Synapsin promoter)

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA 3.1 mitoAURKA

   Plasmid

   #157750

   Purpose

   Expression of AURKA targeted to mitochondria

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Expression

   Mammalian

   Mutation

   Aurora A without the first 30AA + MTS of CoxVIII

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV.CMV.SV40.THBS1-HA-KDEL.SV40(polyA)

   Plasmid

   #156164

   Purpose

   Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus and KDEL (ER retention) sequence

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   Thrombospondin-1 (Thbs1 Mouse)
   

   Use

   AAV

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   THBS1 with addition of ER-retention sequence KDEL

   Promoter

   CMV

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a(+) NRP1 b1b2 (273-586)

   Plasmid

   #162733

   Purpose

   Bacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

   Depositor

   Brett Collins

   Article

   Daly et al Science. 2020 Oct 20. pii: science.abd30

   Insert

   Human NRP1 b1b2 domains (residues 273-586)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Codon optimised

   Available Since

   March 29, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V

   Plasmid

   #119723

   Purpose

   AAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Thomas Oertner

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Inserts

   CaMPARI2
   CaMPARI2
   

   Use

   AAV

   Tags

   NES-his

   Expression

   Mammalian

   Mutation

   F391W, L398V

   Promoter

   hSyn1

   Available Since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-IRES-hrGFP-mWnt3a

   Plasmid

   #89771

   Purpose

   Expresses mouse Wnt3a in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Article

   Rodriguez et al J Neurosci. 2014 Jul 30;34(31):10285-97.

   Insert

   mouse Wnt3a (Wnt3a Mouse)
   

   Use

   AAV

   Tags

   None

   Promoter

   CMV

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO-Tet-On-ARNTL-shRNA3

    Plasmid

    #166915

    Purpose

    Lentivirus for inducible knockdown of human ARNTL(BMAL1)

    Depositor

    Roland Friedel

    Article

    Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

    Insert

    ARNTL shRNA (BMAL1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-hSyn-EGFP-CAAX

    Plasmid

    #208684

    Purpose

    Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    membrane-localized enhanced green fluorescent protein EGFP-CAAX
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AP1muA single guide RNA

    Plasmid

    #230030

    Purpose

    guide RNA for AP1muA tagging at the C-terminus in pX459

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

    Insert

    AP1M1 guide (AP1M1 Human)
    

    Use

    CRISPR

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-TRE3_ mTFP1- Vamp2

    Plasmid

    #201214

    Purpose

    AAV expression of TRE(TRE3G)-driven, Vamp2-conjugated mTFP1 expression for fluorescent labling of axon terminals

    Depositor

    Tetsuo Yamamori

    Article

    Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

    Insert

    mTFP1 (from addgene #54613)
    

    Use

    AAV

    Tags

    mouse Vamp2

    Expression

    Bacterial and Mammalian

    Promoter

    TRE3G

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-PPO-Venus

    Plasmid

    #139502

    Purpose

    Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Insert

    parapinopsin
    

    Tags

    Venus tag following 3x Ala linker

    Expression

    Mammalian

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TBK1 gRNA (BRDN0001148392)

    Plasmid

    #76362

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TBK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TBK1 (TBK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAVS1 KanR foundation lox casette

    Plasmid

    #132388

    Purpose

    Foundation cassette containing lox sites for cre-mediated exchange of inducible vectors, confers mammalian G418 resistance, targets AAVS1 locus

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    G418 resistance
    

    Use

    CRISPR and Cre/Lox

    Expression

    Mammalian

    Promoter

    promoterless

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TBK1 gRNA (BRDN0001145663)

    Plasmid

    #76361

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TBK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TBK1 (TBK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-nEF-NpHR3.3-EYFP

    Plasmid

    #137151

    Purpose

    Viral expression of NpHR3.3-EYFP

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    NpHR3.3-EYFP
    

    Use

    AAV

    Mutation

    W179F

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40

    Plasmid

    #101062

    Purpose

    AAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Insert

    CaMPARI2-H396K
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Promoter

    hsyn (synapsin-1)

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-GRAB_CCK1.0

    Plasmid

    #208673

    Purpose

    Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge