We narrowed to 17,876 results for: she

1. pelBHisTEV

   Plasmid

   #157741

   Purpose

   empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites

   Depositor

   Debra Hansen

   Type

   Empty backbone

   Use

   Tags

   PelB signal sequence + His10-tag + TEV protease c…

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AICSDP-39:RAB5A-mEGFP

   Plasmid

   #107579

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human RAB5A

   Depositor

   Allen Institute for Cell Science

   Insert

   RAB5A Homology Arms with mEGFP-linker (RAB5A Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   May 9, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. TC1541A

   Plasmid

   #164875

   Purpose

   Split GFP reporter expressing GFP(beta1-10) following C>U editing of the target site

   Depositor

   Tian Chi

   Article

   Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

   Insert

   Split GFP(beta1-10)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTmpKm

   Plasmid

   #176227

   Purpose

   A template plasmid for amplification of the cI-hok-neo cassette

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   cI of phage lambda, hok of the R1 plasmid, neo
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmTurquoise2-Drp1

   Plasmid

   #110946

   Purpose

   Expresses dynamin-like protein 1 fused to mTurquoise2

   Depositor

   Pablo Rivera-Fuentes

   Insert

   Dynamin-1-like protein (DNM1L Human)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. MECP2 in pENTR

   Plasmid

   #216592

   Purpose

   gateway cloning donor vector containing MECP2

   Depositor

   Douglas Allan, Christopher Loewen

   Insert

   MECP2 (MECP2 Human)
   

   Use

   Gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

   Plasmid

   #159908

   Purpose

   Mutagenesis of Htr2a

   Depositor

   Larry Zweifel

   Insert

   Htr2a (Htr2a Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3-FLAG-SENP3

   Plasmid

   #115521

   Purpose

   Mammalian expression construct

   Depositor

   Martine Roussel

   Article

   Kuo et al Cell Cycle. 2008 Nov 1;7(21):3378-87. do

   Insert

   SENP3 (Senp3 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. DYRK1A

   Plasmid

   #38913

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   DYRK1A (DYRK1A Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   codon-optimized

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pGFP Cas

    Plasmid

    #50729

    Purpose

    mammalian expression of GFP-Cas. Note-This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Insert

    Cas (Bcar1 Rat)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag FoxO3

    Plasmid

    #14937

    Depositor

    Joan Massague

    Article

    Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

    Insert

    FoxO3 (FOXO3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid

    #50470

    Purpose

    Gq-coupled hM3D-IRES-mCitrine under the control of GFAP promoter

    Depositor

    Bryan Roth

    Insert

    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    GFAP

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-CAG-Dest-pA-pgk-bsd

    Plasmid

    #74918

    Purpose

    Gateway destination piggyBac vector

    Depositor

    Jose Silva

    Article

    dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pJCX3-ACTG2_R257C

    Plasmid

    #228911

    Purpose

    Expresses mutant R257C smooth muscle actin gamma-2 in human cells

    Depositor

    Roberto Dominguez

    Insert

    Actin gamma 2, smooth muscle (ACTG2 Human)
    

    Use

    Tags

    HIS-FLAG-TEV

    Expression

    Bacterial and Mammalian

    Mutation

    R257C

    Promoter

    CMV

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJCX3-ACTG2_WT

    Plasmid

    #228910

    Purpose

    Expresses wildtype smooth muscle actin gamma-2 in human cells

    Depositor

    Roberto Dominguez

    Insert

    Actin gamma 2, smooth muscle (ACTG2 Human)
    

    Use

    Tags

    HIS-FLAG-TEV

    Expression

    Bacterial and Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG416-GAL-FUS-G156E

    Plasmid

    #181716

    Purpose

    Galactose inducible expression of FUS-G156E

    Depositor

    Alejandro Chavez

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G156E

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOET5 CVB3-VLP

    Plasmid

    #218146

    Purpose

    Forms coxsackievirus 3-like particle in insect cells by expression of VP1–VP4 polyprotein (GeneID M33854.1) and 3CD protease (GeneID M33854.1).

    Depositor

    Minna Hankaniemi

    Inserts

    VP1–VP4
    3CD
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CMV and Polyhedrin

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTmpSm

    Plasmid

    #176228

    Purpose

    A template plasmid for amplification of the cI-hok-strA cassette

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

    Insert

    cI of phage lambda, hok of the R1 plasmid, strA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lamin A (G465D)-mRFP

    Plasmid

    #124271

    Purpose

    Lamin A mutant (G465D), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.

    Depositor

    Eric Schirmer

    Insert

    LMNA (G465D) (LMNA Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    LMNA (G465D)

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBad-PhoCl2f

    Plasmid

    #164036

    Purpose

    Improved PhoCl variant with faster dissociation rate

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    PhoCl2f
    

    Use

    Tags

    His tag, T7 tag, Xpress Tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBad promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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