We narrowed to 11,125 results for: HA

1. pLenti-PGK-NRAS(G12V)

   Plasmid

   #35632

   Depositor

   Daniel Haber

   Article

   Singh et al Cell. 2012 Feb 17;148(4):639-50.

   Insert

   N-Ras(G12V) (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Glycine 12 to Valine

   Promoter

   PGK

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

2. BII-Sh-FUCCI-Luc

   Plasmid

   #133364

   Purpose

   Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   Clover-Geminin-IRES-mKO2-Cdt1
   

   Tags

   HA-tagged Clover-Geminin

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

   Plasmid

   #162334

   Purpose

   Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   dSaCas9-KRAB
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC93-pmax-dCas9VP160

   Plasmid

   #48225

   Purpose

   dCas9VP160 on pmax expression vector

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP160 activation domain
   

   Use

   CRISPR

   Tags

   HA Tag and VP160

   Expression

   Mammalian

   Mutation

   D10A;H840A (catalytically inactive)

   Promoter

   CAGGS

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNLS-2xBFP(4C)

   Plasmid

   #176151

   Purpose

   Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.

   Depositor

   Siegfried Musser

   Article

   Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

   Insert

   NLS-2xBFP(4C)
   

   Tags

   6xHis tag and SV40 nuclear localization sequence …

   Expression

   Bacterial

   Mutation

   Compared with EGFP, the two BFP domains have the …

   Promoter

   Ptrc

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUHD.NFATc1nuc

   Plasmid

   #40616

   Depositor

   Jerry Crabtree

   Article

   Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

   Insert

   NFATc1nuc (NFATC1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S172A S175A S176A S178A S179A S181A S184A S187A S…

   Promoter

   CMV2

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. Barcode backbone

   Plasmid

   #158665

   Purpose

   This plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combination

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   hcRED

   Expression

   Mammalian

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG_EGFP-GFE3

   Plasmid

   #79869

   Purpose

   Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-GFE3
   

   Use

   Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Promoter

   Beta-actin

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMX IkB alpha M

   Plasmid

   #12329

   Depositor

   Inder Verma

   Article

   Van Antwerp et al Science. 1996 Nov 1. 274(5288):787-9.

   Insert

   IkB alpha M (Nfkbia Mouse)
   

   Expression

   Mammalian

   Mutation

   Dominant-negative mutant. Many signal transductio…

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-CD4-sTC-M13-GFP-P2A-CaM-V5-sTN

    Plasmid

    #219781

    Purpose

    CaST-P2A for HEK cell expression

    Depositor

    Christina Kim

    Article

    Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

    Insert

    CD4-sTC-M13-GFP-P2A-CaM-V5-sTN
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Biomolecular FeedBack (FB)

    Plasmid

    #109391

    Purpose

    This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pJ23100mut-dCas9-T- phtpG1-sgRNA
    

    Expression

    Bacterial

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLL3.7m-psRaf1

    Plasmid

    #89364

    Purpose

    A lenti-viral vector expressing photoswitchable Raf1 in mammalian cells

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2017 Feb 24;355(6327):836-842.

    Insert

    psRaf1 (RAF1 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Human influenza hemagglutinin (HA) tag and pdDron…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. mt-DdCBE G1333-N

    Plasmid

    #174560

    Purpose

    mitochondrial-targeting DdCBE (split DddA, G1333-N) Golden Gate destination vector

    Depositor

    Jin-Soo Kim

    Article

    Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

    Insert

    DdCBE G1333-N
    

    Tags

    HA

    Expression

    Plant

    Promoter

    PcUbi

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYDE009

    Plasmid

    #194151

    Purpose

    Delivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.

    Depositor

    Edward Geisinger

    Article

    Bai et al J Bacteriol. 2021 May 20;203(12):e005652

    Insert

    tetR-tetP-dcas9-rrnB T1-T7Te
    

    Expression

    Bacterial

    Promoter

    tetp

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)

    Plasmid

    #191164

    Purpose

    Similar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequences

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587:EGFP (additional cutsites)
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pM9-BetaGal-8C

    Plasmid

    #172489

    Purpose

    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    M9-BetaGal-8C
    

    Tags

    6xHis and M9 nuclear localization sequence (from …

    Expression

    Bacterial

    Mutation

    BetaGal has two cysteine mutations (C77A, C1022S)…

    Promoter

    T7

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. dCas9-NS3-NLS/VPR

    Plasmid

    #112244

    Purpose

    Encodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.

    Depositor

    John Ngo

    Article

    Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

    Insert

    dCas9-NS3-NLS/VPR
    

    Tags

    AU1 (N term on NS3) and HA (C term on NS3)

    Expression

    Mammalian

    Mutation

    NS3 mutant T54A

    Promoter

    CMV

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. p6610 MSCV-IP N-HAonly TIMP1

    Plasmid

    #35009

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    TIMP1 (TIMP1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    MSCV LTR

    Available Since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a

    Plasmid

    #31483

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 2.1 (V2468 zfn target) EF Puro
    

    Use

    Lentiviral

    Mutation

    Traffic Light 2.0 series have a +3 PEST sequence …

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. p6592 MSCV-IP N-HAonly WWP2

    Plasmid

    #34905

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    WWP2 (WWP2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    MSCV LTR

    Available Since

    Feb. 8, 2012

    Availability

    Academic Institutions and Nonprofits only