We narrowed to 11,125 results for: HA

1. pcDNA.5.1-FHS-TNRC6A-SD-S1332A/S1346A

   Plasmid

   #215907

   Purpose

   Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A mutation

   Depositor

   Kumiko Ui-Tei

   Article

   Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

   Insert

   TNRC6A (TNRC6A Human)
   

   Tags

   Flag-HA-SBP

   Expression

   Mammalian

   Mutation

   contain aa1224-1709, S1332A, S1346A

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA.5.1-FHS-TNRC6A-SD-S1616D/S1691D

   Plasmid

   #215908

   Purpose

   Expression of TNRC6A-silencing domain (SD) with S1616D/S1691D mutation

   Depositor

   Kumiko Ui-Tei

   Article

   Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

   Insert

   TNRC6A (TNRC6A Human)
   

   Tags

   Flag-HA-SBP

   Expression

   Mammalian

   Mutation

   contain aa1224-1709, S1616D, S1691D

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAVS1_dCas9-XTEN-KRAB-p2A-mCherry

   Plasmid

   #222107

   Purpose

   Donor vector for CRISPRi integration at AAVS1 with an mCherry fluorophore

   Depositor

   Jonathan Weissman

   Article

   She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

   Insert

   CRISPRi-kox1(KRAB) (cas9 Human, S. pyogenes)
   

   Use

   AAV and CRISPR

   Tags

   HA and P2A-mCherry

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Stargazin-EBFP2-FRB

   Plasmid

   #222417

   Purpose

   To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells

   Depositor

   Stephen Royle

   Article

   Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

   Insert

   Cacng2 (Cacng2 Mouse)
   

   Tags

   EBFP2-HA-FRB

   Expression

   Mammalian

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. dCas9-FCPF

   Plasmid

   #220131

   Purpose

   Expresses dCas9-FCPF in mammalian cells

   Depositor

   Xinlai Cheng

   Article

   Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

   Insert

   dCas9-FCPF (cas9 S. pyogenes, Synthetic)
   

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAVS1_dCas9-XTEN-KRAB-p2A-GFP

   Plasmid

   #222108

   Purpose

   Donor vector for CRISPRi integration at AAVS1 with a GFP fluorophore

   Depositor

   Jonathan Weissman

   Article

   She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

   Insert

   CRISPRi-kox1(KRAB) (cas9 Human, S. pyogenes)
   

   Use

   AAV and CRISPR

   Tags

   HA and P2A-GFP

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Available Since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEM-601[insert(+1) TA-rich]

   Plasmid

   #221196

   Purpose

   601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601

   Depositor

   Gregory Bowman

   Article

   Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

   Insert

   601[insert(+1)TA-rich]
   

   Use

   Unspecified

   Mutation

   601 has an additional mutation added 22 bp from d…

   Available Since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNS49 (enAsCas12a-KRAB piggyBac)

   Plasmid

   #217335

   Purpose

   piggyBac expresion of enAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   enAsCas12a
   

   Use

   CRISPR

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   E174R/S542R/K548R

   Promoter

   CAG

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDY279

   Plasmid

   #182959

   Purpose

   Str resistant,CoE1* ori. CRISPR/Cas9 gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations in ptsI. (for 1st round editing)

   Depositor

   Shelley Copley

   Article

   Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

   Insert

   gRNA (ttcaggcattttagcatccc), homologous repair template for ptsI
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDY281

    Plasmid

    #182960

    Purpose

    Amp resistant, CoE1* ori. CRISPR/Cas9 induced by AHTc, gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations of ptsI. (for 2nd round editing)

    Depositor

    Shelley Copley

    Article

    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

    Insert

    gRNA (acctggtgaagccaatattc), homologous repair template for ptsI
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FKBP12-CB2mutx2-mTagBFP2-HAx2

    Plasmid

    #207147

    Purpose

    Tandem mutant calcium-buffer with rapalog dimerizer

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    Calbindin-2 (CALB2 Human)
    

    Tags

    FKBP12, HA, and mTagBFP2

    Expression

    Mammalian

    Mutation

    E40Q, E87Q, E131Q, E175Q, E219Q

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 185_pETcon_SARS2_BQ1-1

    Plasmid

    #208085

    Purpose

    yeast surface display of the SARS-CoV-2 BQ.1.1 variant RBD

    Depositor

    Tyler Starr

    Article

    Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

    Insert

    SARS-CoV-2 BQ.1.1 RBD (S SARS-CoV-2 virus, Budding Yeast)
    

    Tags

    HA and c-Myc

    Expression

    Yeast

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 186_pETcon_SARS2_XBB1-5

    Plasmid

    #208086

    Purpose

    yeast surface display of the SARS-CoV-2 XBB.1.5 variant RBD

    Depositor

    Tyler Starr

    Article

    Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

    Insert

    SARS-CoV-2 XBB.1.5 RBD (S SARS-CoV-2 virus, Budding Yeast)
    

    Tags

    HA and c-Myc

    Expression

    Yeast

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcS-RDG-C1C2

    Plasmid

    #200163

    Purpose

    This plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.

    Depositor

    Masako Harada

    Article

    Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)

    Inserts

    RDG-C1C2
    C1C2 domain of lactadherin
    

    Tags

    HA and HIS

    Expression

    Mammalian

    Mutation

    sequence adopted from doi: 10.1371/journal.pone.0…

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLVX-CALPAIN-7-mCherry, F98D

    Plasmid

    #180653

    Purpose

    Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. F98D mutation. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-53.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRNA resistant to: GCACCCAUACCUUUACAUU

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCA528 RPS6KL1 MIT, residues 20-140

    Plasmid

    #180623

    Purpose

    Bacterial expression for RPS6KL1 MIT domain, residues 20-140. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-32.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    RPS6KL1 (RPS6KL1 Human)
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 20-140

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-THTAP-SNX15, residues 263-342

    Plasmid

    #180624

    Purpose

    Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-GST tag. Internal ID:WISP21-130.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SNX15 (SNX15 Human)
    

    Tags

    HIS-GST

    Expression

    Bacterial

    Mutation

    Residues 263-342

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCA528 SNX15 MIT residues 263-342

    Plasmid

    #180625

    Purpose

    Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-SUMO tag. Internal ID:WISP21-14

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SNX15 (SNX15 Human)
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 263-342

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCA528 USP8, residues 1-147

    Plasmid

    #180636

    Purpose

    Bacterial expression for USP8 MIT domain, residues 1-147. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-86.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    USP8 (USP8 Human)
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-147

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET16B VPS4A, residues 1-84

    Plasmid

    #180638

    Purpose

    Bacterial expression for VPS4A MIT domain residues 1-84. Has N-terminal HIS-TAG. Internal ID: WISP05-43

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    VPS4A (VPS4A Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Residues 1-84

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge