We narrowed to 5,290 results for: phy
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Plasmid#63836PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
ExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK511
Plasmid#71591PurposeProduces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag, His222>Gln mutant (TpCSH6-H222Q)DepositorInsertcitrate synthase
TagsHis6ExpressionBacterialMutationchanges histidine-222 to glutaminePromoterT7 promoterAvailable SinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
S. aureus DHPS
Plasmid#64079PurposeExpression of Staphylococcus aureus dihydropteroate synthaseDepositorInsertdihydropteroate synthase
TagsHistidine tagExpressionBacterialAvailable SinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA5woH
Plasmid#65019PurposeExpresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureusDepositorInsertsortase A
TagsS tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, D160N, D165A, K19…Promotertetracycline promoter/operatorAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrG_B1-DK
Plasmid#117981PurposeSdrG B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B1
TagsHIS, sortase, and ybbrExpressionBacterialPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrG_B2-DK
Plasmid#117982PurposeSdrG B2 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B2
TagsHIS, sortase, and ybbrExpressionBacterialPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA
Plasmid#61592PurposeA human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).DepositorHas ServiceAAV6 and AAV8InserthSaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianAvailable SinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22b_EcSSB_1
Plasmid#78203PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (G26C)
ExpressionBacterialMutationG26C (please see depositor comment below)PromoterT7Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Fos DD
Plasmid#8968DepositorAvailable SinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28-SaCas9
Plasmid#178901PurposeSaCas9 (Cas9 from Staphylococcus aureus)DepositorInsertSaCas9
TagsHis6-Tag, bipartite nuclear localization signal f…ExpressionBacterialAvailable SinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJAB988
Plasmid#133920PurposeminiICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoterDepositorInsertminiICE-tet(M)cat(PC194)_PT7-GFP
UseSynthetic BiologyExpressionBacterialPromoterT7 promoterAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
gWiz_BM40-vGAT-VL-hIgG1-CL
Plasmid#218437Purposemammalian expression plasmid for the light chain fragment of an anti-vGAT Fab domainDepositorInsertvGAT-VL-hIgG1-CL
UseAffinity Reagent/ AntibodyTagsBM40ExpressionMammalianPromoterCMVAvailable SinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFSW GAP43-jRGECO1a P2A Syp-Halo
Plasmid#185565PurposeExpression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequenceDepositorInsertGAP43-jRGECO1a P2A Syp-Halo
UseLentiviralTagsHaloTagAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPgiCas13b
Plasmid#184833PurposeInducible expression of PgiCas13b nuclease in bacteria.DepositorInsertCas13b nuclease from Porphyromonas gingivalis
UseCRISPRExpressionBacterialPromoterT7 promoterAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA7
Plasmid#64986PurposeExpresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, E105K, E108A, D16…Promotertetracycline promoter/operatorAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSNRW-Z5-Nluc
Plasmid#174487PurposeRecombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.DepositorInsertZ5-Nluc
Tags6x Histidine tag (N terminal on insert)ExpressionBacterialPromoterT7lac promoterAvailable SinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
114_UBI_iCre_crystGFP
Plasmid#171797PurposeTol2-plasmid ubiquitously expressing iCre (ubiquitin promoter) along with eGFP selection marker (Crystallin promoter -Lens/Eyes-)DepositorInsertoptimised iCre under the control of the Ubiquitin promoter
PromoterUbiquitinAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH47802-p35S::tdTomato-ER::tNOS
Plasmid#160220PurposetdTomato fluorescent protein-ER localization, 35 promoter, tNOS terminator, transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 1 reverseDepositorInsertp2x35S::TdTomato-ER::tNOS
UseSynthetic Biology; Moclo toolkit level 1 acceptor…ExpressionPlantAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC0067 PguCas13 (B11) His6-TwinStrep-SUMO-BsaI
Plasmid#115218PurposeExpresses PguCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPguCas13
TagsSUMOExpressionBacterialAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZA26
Plasmid#158504PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-4xMyc. Km plant selection marker.DepositorAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only