We narrowed to 61,282 results for: sap

1. pLenti-uORF1-MET

   Plasmid

   #214104

   Purpose

   MET 5'UTR upstream of mGFP (uORF1 mutated)

   Depositor

   Paolo Comoglio

   Article

   Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

   Insert

   MET 5'UTR
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   uORF1 mutation

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDual_dsCas9_Puro_hs_NC_chr1

   Plasmid

   #214688

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

   Depositor

   Kevin Janes

   Insert

   dgRNA_Chr1
   

   Use

   CRISPR and Lentiviral

   Mutation

   Puromycin resistance cassette has silent mutation…

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDual_dsCas9_Puro_hs_TP73

   Plasmid

   #214689

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human TP73

   Depositor

   Kevin Janes

   Insert

   dgRNA_TP73 (TP73 Human)
   

   Use

   CRISPR and Lentiviral

   Mutation

   Puromycin resistance cassette has silent mutation…

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAcGFP-C1-DGKd2

   Plasmid

   #224897

   Purpose

   Express N-terminal mCherry fused to human diacylglycerol kinase delta isoform 2 in mammalian cells

   Depositor

   Chiaki Murakami

   Insert

   DGKD (DGKD Human)
   

   Tags

   AcGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed

   Plasmid

   #226590

   Purpose

   Pharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.

   Depositor

   D. James Surmeier

   Insert

   PSAML-141F,Y115F-GlyR
   

   Use

   AAV

   Tags

   P2A-Fusion Red

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Rab11A[8-175]-His-C Q70L

   Plasmid

   #228042

   Purpose

   Protein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling. Constitutively active mutation (Q70L).

   Depositor

   Wade Harper

   Insert

   Rab11A[8-175]-His-C Q70L (RAB11A Human)
   

   Expression

   Bacterial

   Mutation

   Q70L

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-ITR2-H1-sgCTG-SlugD10A-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #222857

   Purpose

   This plasmid codes for the Slug-KH nickase with a sg(CTG)6

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   Slug-KHD10A+sgCTG
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, ITR2 and ITR2

   Expression

   Mammalian

   Mutation

   KH variant and D10A mutation

   Promoter

   H1 and eCMV

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57-ITR2-H1-sgAGC-SlugD10A-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #222859

   Purpose

   This plasmid codes for the Slug-KH nickase with a sg(AGC)6

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   Slug-KHD10A+sgAGC
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, ITR2 and ITR2

   Expression

   Mammalian

   Mutation

   KH variant and D10A mutation

   Promoter

   H1 and eCMV

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC57-ITR2-H1-sgCAG-SlugD10A-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #222858

   Purpose

   This plasmid codes for the Slug-KH nickase with a sg(CAG)6

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   Slug-KHD10A+sgCAG
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, ITR2 and ITR2

   Expression

   Mammalian

   Mutation

   KH variant and D10A mutation

   Promoter

   H1 and eCMV

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC57-ITR2-H1-sgGCA-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222860

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(GCA)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgGCA
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-cT3G-cERP2-MYBL1

    Plasmid

    #222547

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of MYBL1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    MYBL1 (MYBL1 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-cT3G-cERP2-SMAD4

    Plasmid

    #222573

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of SMAD4

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    SMAD4 (SMAD4 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-cT3G-cERP2-ZNF541

    Plasmid

    #222560

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of ZNF541

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    ZNF541 (ZNF541 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-cT3G-cERP2-NFYB

    Plasmid

    #222561

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of NFYB

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    NFYB (NFYB Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. N-SF-TAP-DEST-TLK2-D592V

    Plasmid

    #218835

    Purpose

    Expresses N-terminal Strep-FLAG-tagged human TLK2 mutant D592V (kinase dead).

    Depositor

    Travis Stracker

    Insert

    TLK2 Q592V (kinase dead) (TLK2 Human)
    

    Tags

    FLAG-STREP

    Expression

    Mammalian

    Mutation

    Q592V

    Promoter

    CMV

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAcGFP-N1_CBPdIDR7-H1

    Plasmid

    #221930

    Purpose

    Transient expression of GFP-tagged CBPdeltaIDR7-H1 in mammalian cells

    Depositor

    Daniel Bose

    Insert

    CBPdeltaIDR7-H1 (CREBBP Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deletion of IDR7, CBP aa 2187 - 2216

    Available Since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAcGFP-N1_CBPdCFID

    Plasmid

    #221909

    Purpose

    Transient expression of GFP-tagged CBPdeltaCFID in mammalian cells

    Depositor

    Daniel Bose

    Insert

    CBPdeltaCFID (CREBBP Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deletion of the CBP-FUS interaction domain (CFID)…

    Available Since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAcGFP-N1_CBPdIDR6-H2

    Plasmid

    #221929

    Purpose

    Transient expression of GFP-tagged CBPdeltaIDR6-H2 in mammalian cells

    Depositor

    Daniel Bose

    Insert

    CBPdeltaIDR6-H2 (CREBBP Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deletion of IDR6, CBP aa 1951 - 1978

    Available Since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOCC120-MBP-DDX3X(D354V)-monoGFP-His

    Plasmid

    #219986

    Purpose

    DDX3X(D354V) protein purification

    Depositor

    Simon Alberti

    Insert

    DDX3X(D354V) (DDX3X Human)
    

    Tags

    MBP and monoGFP and 6xHis

    Expression

    Insect

    Mutation

    D354V

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOCC120-MBP-DDX3X(G325E)-monoGFP-His

    Plasmid

    #219987

    Purpose

    DDX3X(G325E) protein purification

    Depositor

    Simon Alberti

    Insert

    DDX3X(G325E) (DDX3X Human)
    

    Tags

    MBP and monoGFP and 6xHis

    Expression

    Insect

    Mutation

    G325E

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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