We narrowed to 11,125 results for: HA

1. pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11

   Plasmid

   #193752

   Purpose

   human codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGH

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   EF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA
   

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. VCP(A232E)-EGFP

   Plasmid

   #23973

   Depositor

   Nico Dantuma

   Article

   Tresse et al Autophagy. 2010 Feb 22. 6(2):.

   Insert

   Valosin containing protein (VCP Human)
   

   Tags

   6xHis, EGFP, and HA

   Expression

   Mammalian

   Mutation

   A232E

   Available Since

   Feb. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. IMPT-10316:GABR1

   Plasmid

   #157850

   Purpose

   HA-TEV-VFT-TMD-CC-PreX-eGFP in pFastBac1 for Sf9 expression

   Depositor

   Vadim Cherezov, Raymond Stevens

   Article

   Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

   Insert

   GABR1 (GABBR1 Human)
   

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. EP-Pol vector

   Plasmid

   #131686

   Purpose

   A lentiviral vector that constitutively expresses an error-prone variant of the adenovirus DNA polymerase

   Depositor

   Matthew D. Shoulders

   Article

   Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

   Insert

   Adenovirus DNA polymerase
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   F421Y, D827A

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Wild-type AdPol vector

   Plasmid

   #131685

   Purpose

   A lentiviral vector that constitutively expresses the wild-type adenovirus DNA polymerase

   Depositor

   Matthew D. Shoulders

   Article

   Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

   Insert

   Adenovirus DNA polymerase
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   June 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGV15 (CK2alpha K68M)

   Plasmid

   #27089

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2alpha K68M (kinase dead) (CSNK2A1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K68M (kinase dead)

   Promoter

   CMV

   Available Since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

7. 011LP-pAAV-TNT4-EmGFP-pA

   Plasmid

   #120280

   Purpose

   AAV vector for cardiomyocyte specific expression of Emerald Green Fluorescent Protein using cardiac troponin T promoter

   Depositor

   Xander Wehrens

   Article

   Ni et al Circ Res. 2019 Jan 18;124(2):256-262. do

   Insert

   EmGFP
   

   Use

   AAV

   Tags

   3XFlag and HA

   Promoter

   TNT Cardiac Troponin T promoter

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2TAL3-RR

   Plasmid

   #37276

   Purpose

   TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)

   Depositor

   David Grunwald

   Article

   Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

   Type

   Empty backbone

   Tags

   C terminal Tal From pTAL3, FokI RR, HA, and N-ter…

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-FLAG-CCR5

   Plasmid

   #98949

   Purpose

   Mammalian expression plasmid for N-terminal FLAG-tagged human CCR5

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CCR5 (CCR5 Human)
   

   Tags

   FLAG (dykdddd) epitope tag and Signal/leader sequ…

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid

    #84240

    Purpose

    Expresses GA-inducible VPR-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV5 MEKK3 WT

    Plasmid

    #12186

    Depositor

    Gary Johnson

    Article

    Blank et al J Biol Chem. 1996 Mar 8. 271(10):5361-8.

    Insert

    MEKK3 (Map3k3 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pGL4 mIL-17 2kb RORgamma-t mut

    Plasmid

    #20127

    Depositor

    Warren Strober

    Article

    Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

    Insert

    mIL-17 (Il17a Mouse)
    

    Use

    Luciferase

    Mutation

    RORgamma-t binding site in 0.9kb of 2kb promoter …

    Available Since

    Jan. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pCEP4-myc-PDGFRalpha

    Plasmid

    #136434

    Purpose

    Mammalian expression plasmid for c-myc-tagged PDGFRA

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    PDGFRalpha (PDGFRA Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLpA-DN.JNK2

    Plasmid

    #51943

    Purpose

    Adenoviral vector for dominant negative JNK2 expression

    Depositor

    Jeffery Molkentin

    Article

    Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

    Insert

    DN JNK2 (Mapk9 Mouse)
    

    Use

    Adenoviral

    Tags

    HA

    Mutation

    dominant negative: the dual phosphorylation moti…

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pMJ923

    Plasmid

    #78313

    Purpose

    Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli

    Depositor

    Martin Jinek

    Article

    Burger et al Development. 2016 Apr 29. pii: dev.13480

    Insert

    SpCas9
    

    Tags

    HA-2xNLS-mCherry-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    human codon-optimized gene sequence

    Promoter

    T7

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-EF1-FHC-POLQ-DM

    Plasmid

    #64880

    Purpose

    lentiviral expression of human POLQ double mutant (in POL and HDL domains)

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    K121M and D2330A,Y2331A

    Promoter

    EF1alpha

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCSF107mT-GATEWAY-3'-3HA

    Plasmid

    #67616

    Purpose

    A pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning technology

    Depositor

    Todd Stukenberg

    Article

    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

    Type

    Empty backbone

    Use

    In vitro transcription or translation off of an s…

    Tags

    three inframe 3' HA tags

    Expression

    Mammalian

    Promoter

    CMV and SP6

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pAC2-dual-dCas9VP48-sgExpression

    Plasmid

    #48236

    Purpose

    Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9VP48
    

    Use

    CRISPR

    Tags

    HA-tag and VP48

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBADHISTEV_PfeE

    Plasmid

    #129120

    Purpose

    pBADHISTEV carrying pfeE for overexpression of PfeE in E. coli

    Depositor

    James Naismith

    Article

    Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

    Insert

    Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
    

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    PBAD

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. POLArISact

    Plasmid

    #164971

    Purpose

    Expresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cells

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    POLArISact
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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