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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,290 results for: phy

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Showing: 921 - 940 of 5290 results

  1. MSP2262

    Plasmid
    #70703
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A & H557A in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70703-plasmid-map-sequence-id-127765

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  3. MSP2279

    Plasmid
    #70706
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A and H557A in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70706-plasmid-map-sequence-id-127768

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  5. pmirGLO-Smad4-1719

    Plasmid
    #49383
    Purpose
    Insert is a fragment of the Smad4 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.
    Depositor
    Martine Roussel
    Article
    Murphy et al Cancer Res. 2013 Oct 21.
    Insert
    Smad family member 4 (SMAD4 Human, Mouse)
    Use
    Luciferase
    Promoter
    Human phosphoglycerate kinase (PGK) promoter
    Available Since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    49383-plasmid-map-sequence-id-73406

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  7. pmirGLO-Smad5-1719

    Plasmid
    #49382
    Purpose
    Insert is a fragment of the Smad5 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.
    Depositor
    Martine Roussel
    Article
    Murphy et al Cancer Res. 2013 Oct 21.
    Insert
    Smad5 (Smad5 Human, Mouse)
    Use
    Luciferase
    Promoter
    Human phosphoglycerate kinase (PGK) promoter
    Available Since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    49382-plasmid-map-sequence-id-73405

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  14. SAV2137

    Plasmid
    #255512
    Purpose
    DERA enzyme production under control of T7 promoter and inducible lac operator
    Depositor
    Radhakrishnan Mahadevan
    Article
    Kim et al J Biol Chem. 2020 Jan 10;295(2):597-609.
    Insert
    2-deoxyribose-5-phosphate aldolase (DERA) from Staphylococcus aureus
    Tags
    6xHis tag-TEV protease recognition site (ENLYFQ/G…
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    255512-plasmid-map-sequence-id-509487

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  15. p305N-30

    Plasmid
    #246313
    Purpose
    Evaluation of PtU6.2c3 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    PtU6.2c3 promoter
    Use
    CRISPR
    Expression
    Plant
    Mutation
    deletion: -T (-29 from TSS) within TATA
    Promoter
    PtU6.2c3 promoter
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246313-plasmid-map-sequence-id-491198

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  16. p305N-31 (nonfunctional)

    Plasmid
    #246314
    Purpose
    Evaluation of PtU6.2c4 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    PtU6.2c4 promoter (nonfunctional)
    Use
    CRISPR
    Expression
    Plant
    Mutation
    deletions: -A (-41 from TSS), -T (-30), -T (-29);…
    Promoter
    PtU6.2c4 promoter (nonfunctional)
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246314-plasmid-map-sequence-id-491197

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  17. p305N-9 (nonfunctional)

    Plasmid
    #246292
    Purpose
    Evaluation of AtU6.29c13 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    AtU6.29c13 promoter (nonfunctional)
    Use
    CRISPR
    Expression
    Plant
    Mutation
    deletions: -A (-58 from TSS), -T (-57) within USE
    Promoter
    AtU6.29c13 promoter (nonfunctional)
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246292-plasmid-map-sequence-id-491207

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  18. p305N-17 (nonfunctional)

    Plasmid
    #246300
    Purpose
    Evaluation of HbU6.2m3 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    HbU6.2m3 promoter (nonfunctional)
    Use
    CRISPR
    Expression
    Plant
    Mutation
    C-to-T (-29 from TSS), G-to-A (-24)
    Promoter
    HbU6.2m3 promoter (nonfunctional)
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246300-plasmid-map-sequence-id-491174

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  19. p305N-4 (nonfunctional)

    Plasmid
    #246287
    Purpose
    Evaluation of AtU6.1c1 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    AtU6.1c1 promoter (nonfunctional)
    Use
    CRISPR
    Expression
    Plant
    Mutation
    G-to-C at -15 from TSS
    Promoter
    AtU6.1c1 promoter (nonfunctional)
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246287-plasmid-map-sequence-id-491143

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  20. p305N-26 (nonfunctional)

    Plasmid
    #246309
    Purpose
    Evaluation of MtU6.6m7 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    MtU6.6m7 promoter (nonfunctional)
    Use
    CRISPR
    Expression
    Plant
    Mutation
    C-to-A (-63 from TSS), T-to-G (-57)
    Promoter
    MtU6.6m7 promoter (nonfunctional)
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246309-plasmid-map-sequence-id-491101

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Showing: 921 - 940 of 5290 results