We narrowed to 11,125 results for: HA

1. pEAK-Flag/INPP4B

   Plasmid

   #24324

   Depositor

   Lewis Cantley

   Article

   Gewinner et al Cancer Cell. 2009 Aug 4. 16(2):115-25.

   Insert

   Inositol Polyphosphate 4-Phosphatase Type II (INPP4B Human)
   

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   none

   Available Since

   May 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.GFAP.SV40.Thbs1-HA.SV40(polyA)

   Plasmid

   #195552

   Purpose

   Expresses Thbs1 under control of short GFAP promoter

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   Thrombospondin-1 (Thbs1 Mouse)
   

   Use

   AAV

   Tags

   HA-tag

   Expression

   Mammalian

   Promoter

   short GFAP

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSIIpuro-TGFa-ScNeo

   Plasmid

   #209895

   Purpose

   To monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   TGFa-ScNeo (TGFA Human)
   

   Use

   Lentiviral

   Tags

   GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CaMKIIa-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

   Plasmid

   #131003

   Purpose

   bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of CamKIIa promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   Kv2.1-HA

   Promoter

   CaMKIIa

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3/er-(n2)oxStayGold(c4)

   Plasmid

   #185822

   Purpose

   A soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.

   Depositor

   Atsushi Miyawaki

   Article

   Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

   Insert

   er-(n2)oxStayGold(c4)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.3-hALDOA

   Plasmid

   #210809

   Purpose

   For transient transfection

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. plenti-UBC- IRF4-3xHA-pGK-PUR

   Plasmid

   #107386

   Purpose

   Lentiviral expression of IRF4-3xHA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IRF4 (IRF4 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV BARK D110A p2A mCherry

   Plasmid

   #117690

   Purpose

   CMV-iBARK(D110A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs D110A peptide
   

   Tags

   HA / FLAG

   Expression

   Mammalian

   Mutation

   D110A

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. scaffold-linker-U6

   Plasmid

   #89639

   Purpose

   construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

   Depositor

   Wensheng Wei

   Article

   Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBUN6A11

    Plasmid

    #50579

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-VP64
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Mutation

    dCas9 = defective in DNA cleavage; 4×minimal VP16…

    Promoter

    OsU3p and Ubi1p

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBUN6I11

    Plasmid

    #50580

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-KRAB
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Mutation

    dCas9 that is defective in DNA cleavage; the maiz…

    Promoter

    OsU3p and Ubi1p

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBiFC-PBZ-VC

    Plasmid

    #110648

    Purpose

    expresses a PAR-binding (PBZ) domain fused to venus C-terminus

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

    Insert

    PBZ domain from APLF, amino acids 371-451 (APLF Human)
    

    Tags

    HA and VC

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-ALK2 wt

    Plasmid

    #80870

    Purpose

    mammalian expression of ALK2 WT

    Depositor

    Aristidis Moustakas

    Article

    Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

    Insert

    ALK2 (ACVR1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pTBRII / CEP-Zeo / Hygro

    Plasmid

    #16622

    Depositor

    Bert Vogelstein

    Article

    Zhou et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Insert

    TGF beta type II receptor (TGFBR2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

15. GFP(N-glyc)-Tac-TC

    Plasmid

    #162496

    Purpose

    Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in the N-terminus in mammalian cells, and there is one N-glycosylation site in GFP tag.

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    partial length Tac (TMD and cytosolic tail) (IL2RA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Tac is in partial length with its TMD and cysotol…

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid

    #84261

    Purpose

    Expresses AND gate ABA/GA-inducible VPR-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9
    PYL1-VPR-p2a-GID1-ABI
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), ABI, GAI, GID1, HA tag, PYL1, p2a, …

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1

    Plasmid

    #194279

    Purpose

    Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

    Inserts

    humanized VP64 dSaCas9 VP64 T2A Thy1.1
    gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hU6 and hUbC

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC94-pmax-dCas9VP160-2A-puro

    Plasmid

    #48226

    Purpose

    dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
    

    Use

    CRISPR

    Tags

    2A-puro, HA Tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT2/sh-Col1a1/GFP4_Seq1.2

    Plasmid

    #201403

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Expression

    Mammalian

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ10871

    Plasmid

    #183962

    Purpose

    U6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Inserts

    dCas12a(wildtype)
    poly-crRNA array targeting Sox2-Klf4-Oct4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG and U6

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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