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pAVEC1_N-StrepIII

Plasmid

#231276

Purpose

For stably expressing fusion proteins with N terminal StrepIII tags in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

StrepIII

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC1_C-mTurquoise2

Plasmid

#231278

Purpose

For stably expressing fusion proteins with C terminally tagged mTurquoise2 in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mTurquoise2

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC1_C-mNeonGreen

Plasmid

#231280

Purpose

For stably expressing fusion proteins with C terminally tagged mNeonGreen in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mNeonGreen

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC1_C-mScarletI

Plasmid

#231282

Purpose

For stably expressing fusion proteins with C terminally tagged mScarletI in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mScarletI

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC1_C-StrepIII

Plasmid

#231284

Purpose

For stably expressing fusion proteins with C terminal StrepIII tags in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

StrepIII

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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ISA179: Tagatose pathway

Plasmid

#241968

Purpose

Phagemid for the tagatose pathway. Contains p15A, CmR, pac site, AraC, C1, and pBAD.

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

Tagatose pathway

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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POC1553

Plasmid

#238470

Purpose

pMOBC360_VL: Vector Left (VL) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pART15-asB539

Plasmid

#127394

Purpose

Expresses amcyan fluorescent gene and sRNA nt 539-559

Depositor

Ryan Senger

Article

Tanniche et al Biotechnol Prog. 2023 May-Jun;39(3):e332

Insert

AmCyan

Expression

Bacterial

Promoter

PBAD

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pART15-C1

Plasmid

#127395

Purpose

Expresses amcyan fluorescent gene

Depositor

Ryan Senger

Article

Tanniche et al Biotechnol Prog. 2023 May-Jun;39(3):e332

Insert

AmCyan

Expression

Bacterial

Promoter

PBAD

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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POC1554

Plasmid

#238471

Purpose

pMOBC360_VR: Vector Right (VR) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-Lck-mCerulean3

Plasmid

#234538

Purpose

to label the membrane of cells with a fluorescent protein (mCerulean3)

Depositor

Hajime Hirase

Article

Wang et al Anat Sci Int. 2025 Jun 27. doi: 10.1007/

Insert

Lck-mCerulean3

Use

AAV

Mutation

None

Promoter

Short CAG

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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POC1358

Plasmid

#236103

Purpose

Assembled plasmid containing four inserts (from POC1343, POC1344, POC1345 and POC1346) assembled in POC1355

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1525

Plasmid

#226496

Purpose

pMOBC360_VM: Vector Middle (VM) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1551

Plasmid

#226498

Purpose

pMOBK360_StandVKL: Standard Vector (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1552

Plasmid

#226499

Purpose

pMOBC360_StandVC: Standard Vector (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1520

Plasmid

#221581

Purpose

pMOBK360_VR: Vector Right (VR) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1355

Plasmid

#221572

Purpose

Assembly vector designed to be methylated by the M.Osp807II BsaI-associated switch methylase and for the assembly of inserts 1, 2, 3 and 4 from POC1343, POC1344, POC1345 and POC1346; respectively

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1430

Plasmid

#221577

Purpose

Assembly vector designed to be methylated by the M.Osp807II BsaI-associated switch methylase and for the assembly of inserts 1, 2, 3 and 4 from POC1426, POC1427, POC1428 and POC1429; respectively

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1518

Plasmid

#221579

Purpose

pMOBK360_VL: Vector Left (VL) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1519

Plasmid

#221580

Purpose

pMOBK360_VM: Vector Middle (VM) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLB025

Plasmid

#234634

Purpose

pET-28a(+) based plasmid for expression of H16_A1358 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_A1358

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGH916

Plasmid

#230842

Purpose

Ef1a-DivA-BE3-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DivA-BE3

Use

Lentiviral

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH907

Plasmid

#230837

Purpose

Ef1a-MS2-AIDDead-T2A-GFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

MS2-AIDDead

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGH518

Plasmid

#230836

Purpose

Ef1a-MS2-AID*-T2A-GFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

MS2-AID*

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH896

Plasmid

#230840

Purpose

Ef1a-DivA-BE-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DivA-BE

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH397

Plasmid

#230853

Purpose

Ef1a-AID*-n'Cas9-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

AID*-n'Cas9

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH911

Plasmid

#230856

Purpose

Ef1a-BE3-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

BE3 (rAPOBEC1)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH897

Plasmid

#230841

Purpose

Ef1a-nDivA-BE-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

nDivA-BE

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH389

Plasmid

#230847

Purpose

Ef1a-AID*-dCas9-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

AID*-dCas9

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH385

Plasmid

#230848

Purpose

Ef1a-dCas9-AID*-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

dCas9-AID*

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH483

Plasmid

#230835

Purpose

Ef1a-dCas9-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

dCas9

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH811

Plasmid

#230850

Purpose

Ef1a-nDivA-BE-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

nDivA-BE

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH484

Plasmid

#230838

Purpose

Ef1a-AID*-dCas9-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

AID*-dCas9

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH915

Plasmid

#230843

Purpose

Ef1a-BE3-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

BE3

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH880

Plasmid

#230839

Purpose

Ef1a-dCas9-AID*-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

dCas9-AID*

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH720

Plasmid

#230845

Purpose

Ef1a-ABE7.10-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

ABE7.10

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV HA-RIM2

Plasmid

#206151

Purpose

CMV HA-RIM2

Depositor

Erika Holzbaur

Article

Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

Insert

Rim2 (Rims2 Rat)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-LflChmG

Plasmid

#221343

Purpose

Express LflChmG in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmG

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-LflChmH

Plasmid

#221344

Purpose

Express LflChmH in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmH

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-ChmF

Plasmid

#221345

Purpose

Express ChmF in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmF

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-ChmK

Plasmid

#221346

Purpose

Express ChmK in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmK

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-ChmL

Plasmid

#221347

Purpose

Express ChmL in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmL

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-ChmJ

Plasmid

#221342

Purpose

Express ChmJ in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmJ

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pReporter_mNeonGreen

Plasmid

#213838

Purpose

Facilitates genomic integration of the control reporter without zinc finger binding sites.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pReporter_ZF1_mNeonGreen

Plasmid

#213840

Purpose

Facilitates genomic integration of the reporter with upstream binding sites for ZF-1

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pReporter_ZF2_mNeonGreen

Plasmid

#213841

Purpose

Facilitates genomic integration of the reporter with upstream binding sites for ZF-2

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pStorage_Ptet-1_ZF1

Plasmid

#213844

Purpose

CombiGEM storage plasmid for ZF-1 CR fusions.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pStorage_Ptet-2_ZF2

Plasmid

#213845

Purpose

CombiGEM storage plasmid for ZF-2 CR fusions.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEU

Plasmid

#213846

Purpose

LEU integrating plasmid backbone used to create all of the reporter plasmids.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pReporter_TetR

Plasmid

#213847

Purpose

Facilitates genomic integration of tetR expression cassette.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

TetR

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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