We narrowed to 11,632 results for: VARS

1. mCherry-C1-TGAT(C242S,C253S)

   Plasmid

   #84336

   Purpose

   Expresses TGAT tagged with mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

   Insert

   TGAT(C242S,C253S) (TRIO Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C242S, C253S

   Promoter

   CMV

   Available Since

   Dec. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPRv2 puro

   Plasmid

   #98290

   Purpose

   Variant of lentiCRISPRv2 that confers puromycin resistance

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Promoter

   EF-1a

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPRv2 neo

   Plasmid

   #98292

   Purpose

   Variant of lentiCRISPRv2 that confers G418 resistance

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Promoter

   EF-1a

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPRv2 hygro

   Plasmid

   #98291

   Purpose

   Variant of lentiCRISPRv2 that confers hygromycin resistance

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Promoter

   EF-1a

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiCRISPRv2 blast

   Plasmid

   #98293

   Purpose

   Variant of lentiCRISPRv2 that confers blasticidin S resistance

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Promoter

   EF-1a

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-CMV-SOD2-2A-Catalase-WPRE

   Plasmid

   #67635

   Purpose

   AAV vector expressing both SOD2 and mitochondrial targeted Catalase

   Depositor

   Connie Cepko

   Article

   Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

   Insert

   SOD-2A-Catalase (Sod2, Cat Mouse)
   

   Use

   AAV

   Mutation

   Deleted the Peroxisome Targeting Signal from Cata…

   Promoter

   CMV

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-syn-FLEX-jYCaMP1s

   Plasmid

   #135420

   Purpose

   Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

   Depositor

   Kaspar Podgorski

   Article

   Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

   Has Service

   AAV1

   Insert

   jYCaMP1s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-AAV-syn-jGCaMP8s-WPRE

   Plasmid

   #162374

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8s
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S26M F286Y Q315H

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEAT8-137 M1V

   Plasmid

   #173807

   Purpose

   Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells

   Depositor

   Anne Gershenson

   Article

   Krishnan et al Methods Mol Biol. 2017;1639:195-209. doi

   Insert

   SERPiNA1 (SERPINA1 Human)
   

   Expression

   Bacterial

   Mutation

   E1M Otherwise this plasmid encodes the most commo…

   Promoter

   T7

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-CMV-jGCaMP7f

    Plasmid

    #104483

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7f
    

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-AAV-syn-jGCaMP8m-WPRE

    Plasmid

    #162375

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Kif1bβ-pcDNA3

    Plasmid

    #22534

    Depositor

    William Kaelin

    Article

    Schlisio et al Genes Dev. 2008 Apr 1. 22(7):884-93.

    Insert

    Kif1bβ (KIF1B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wt cDNA

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

    Plasmid

    #207878

    Purpose

    AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

    Depositor

    Yeh-Hsing Lao

    Article

    Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)GαsL-260-NLuc

    Plasmid

    #207158

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAS (GNAS Human)
    

    Expression

    Mammalian

    Mutation

    NLuc inserted at position D(260)N

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-EF1a MICA*009-IRES-ZsGreen

    Plasmid

    #114007

    Purpose

    Induces expression of human MICA allele 009 cDNA

    Depositor

    Kai Wucherpfennig

    Article

    Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

    Insert

    MICA*009 (MICA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSIN4-EF2-ABCG2-IRES-Neo

    Plasmid

    #25983

    Depositor

    Ren-he Xu

    Article

    Zeng et al Stem Cells. 2009 Oct . 27(10):2435-45.

    Insert

    ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 (ABCG2 Human)
    

    Use

    Lentiviral

    Tags

    EF1-apha promoter and IRES-Neo

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPICZ-pre-Ost1-alphaf(I)-E2-Crimson

    Plasmid

    #117661

    Purpose

    Test intracellular localisation of E2-Crimson and study the secretion efficiency

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-Ost1-alphaf(I)-E2-Crimson
    

    Expression

    Yeast

    Mutation

    This plasmid encodes the fluorescent protein E2-C…

    Promoter

    pAOX1

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-hSynI-rtTAV16

    Plasmid

    #102367

    Purpose

    AAV vector to drive TET-ON activator expression from human synapsin I promoter

    Depositor

    Tetsuo Yamamori

    Article

    Watakabe et al Front Neural Circuits. 2014 Sep 17;8:110

    Insert

    rtTAV16
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    rtTA variant reported in Gene Therapy (2006) 13, …

    Promoter

    human synapsin I promoter

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP-AAV-syn-jGCaMP8f-WPRE

    Plasmid

    #162376

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCH1/RAD51o

    Plasmid

    #102562

    Purpose

    Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon

    Depositor

    Maria Spies

    Article

    Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11

    Inserts

    Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Human, Synthetic)
    E. coli groE operon
    

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli Expression

    Promoter

    T7

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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