We narrowed to 13,722 results for: crispr cas9

1. pAGM47523

   Plasmid

   #153221

   Purpose

   Level 0 zCas9i vector

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Insert

   SpCas9 with introns and 2 NLS
   

   Use

   Synthetic Biology

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-EGxxFP

   Plasmid

   #50716

   Purpose

   5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.

   Depositor

   Masahito Ikawa

   Article

   Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

   Insert

   split EGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. AZ64_pE.DonorCLYBL.TS

   Plasmid

   #199228

   Purpose

   Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   Expression unit for EGFP reporter
   

   Use

   Gene targeting donor plasmid

   Expression

   Mammalian

   Promoter

   Human PGK1 gene promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSK-Sasg

   Plasmid

   #214350

   Purpose

   Expresses cloning backbone for SlugCas9 sgRNA

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugCas9 tracrRNA
   

   Expression

   Mammalian

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. U6-Nme2_tracr

   Plasmid

   #192146

   Purpose

   Expresses Nme2-sgRNA cloned with BspQI

   Depositor

   Yongming Wang

   Article

   Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

   Insert

   Nme2 sgRNA scaffold
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS423-TyrSgH

   Plasmid

   #163973

   Purpose

   Helper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoter

   Depositor

   Jiazhang Lian

   Article

   Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS423-SgH

   Plasmid

   #163972

   Purpose

   Helper plasmid for cloning gRNAs under the control of the SNR52 promoter.

   Depositor

   Jiazhang Lian

   Article

   Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSK-Spsg

   Plasmid

   #214351

   Purpose

   Expresses cloning backbone for SpCas9 sgRNA

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SpCas9 tracrRNA
   

   Expression

   Mammalian

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS423-ProSgH

   Plasmid

   #163974

   Purpose

   Helper plasmid for cloning gRNAs under the control of the tRNA(Pro) promoter

   Depositor

   Jiazhang Lian

   Article

   Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. UCT1m

    Plasmid

    #121041

    Purpose

    MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    UCT-part guide RNA (Stanley Qi sequence)
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pV1200

    Plasmid

    #111429

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSL1394

    Plasmid

    #129522

    Purpose

    pDEF-SpCas9::GFP, For deletion of pyuis4, PyBiP-minimal promoter, 3rd Generation

    Depositor

    Scott Lindner

    Article

    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

    Insert

    SpCas9
    

    Use

    Plasmodium, rodent-infectious species

    Tags

    3XFLAG-NLS

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEPOR0SP0020

    Plasmid

    #117531

    Purpose

    Level0 Golden Gate part: CDS, SaCas9

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SaCas9 (with stop codon)
    

    Use

    CRISPR

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEPOR0SP0013

    Plasmid

    #117521

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-p

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-p (with stop codon)
    

    Use

    CRISPR

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL1166

    Plasmid

    #129520

    Purpose

    pDEF-SpCas9::GFP, For deletion of pyalba4, PyBiP-full promoter, 2nd Generation

    Depositor

    Scott Lindner

    Article

    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

    Insert

    SpCas9
    

    Use

    Plasmodium, rodent-infectious species

    Tags

    3XFLAG-NLS

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEPOR0SP0021

    Plasmid

    #117532

    Purpose

    Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SaCas9 (no stop codon)
    

    Use

    CRISPR

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL1211

    Plasmid

    #129521

    Purpose

    pDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd Generation

    Depositor

    Scott Lindner

    Article

    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

    Insert

    SpCas9
    

    Use

    Plasmodium, rodent-infectious species

    Tags

    3XFlag-NLS

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEPOR0SP0014

    Plasmid

    #117524

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-KA

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-KA (with stop codon)
    

    Use

    CRISPR

    Mutation

    K855A

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEPOR0SP0009

    Plasmid

    #117522

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-p (no stop codon)
    

    Use

    CRISPR

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICSL90005

    Plasmid

    #117520

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-h (no stop codon)
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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