We narrowed to 19,494 results for: HA

1. pFRT-DHX36iso1

   Plasmid

   #159585

   Purpose

   Express N-ter FLAG HA tagged DHX36 isoform 1 in mammalian cells

   Depositor

   Markus Hafner

   Article

   Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

   Insert

   DHX36 (DHX36 Human)
   

   Tags

   FLAG HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CPSF5

   Plasmid

   #155480

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   CPSF5 (NUDT21 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET42b-BE3

   Plasmid

   #87437

   Purpose

   Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

   Insert

   BE3 (Apobec1 Human, Rat, Bacillus phage PBS2)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3

   Plasmid

   #170163

   Purpose

   Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Has Service

   AAV1

   Insert

   ChroME2s-P2A-H2B-mRuby3
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PRPF4

   Plasmid

   #155850

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   PRPF4 (PRPF4 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Epac-S-H74

   Plasmid

   #170338

   Purpose

   mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Klarenbeek et al PLoS One. 2011 Apr 29;6(4):e19170. doi:

   Insert

   mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHLmMBP-3

   Plasmid

   #72345

   Purpose

   Mammalian expression of secreted N-terminally 8His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Tags

   6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

   Expression

   Mammalian

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1.Gα12-LgB115

   Plasmid

   #134363

   Purpose

   Nanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gα12-LgB115 (GNA12 Human)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Promoter

   T7

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p6600 MSCV-IP N-HAonly JUN

   Plasmid

   #34898

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   JUN (JUN Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   MSCV LTR

   Available Since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1.Gα11-LgB97

    Plasmid

    #134361

    Purpose

    Nanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gα11-LgB97 (GNA11 Human)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    T7

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. -3.6kb human IFN-gamma luc

    Plasmid

    #17599

    Depositor

    Howard Young

    Article

    Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

    Insert

    IFN gamma promoter (IFNG Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    -3.6 kb to +70 of promoter

    Available Since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p6608 MSCV-IP N-HAonly CHAF1B

    Plasmid

    #34901

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    CHAF1B (CHAF1B Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    MSCV LTR

    Available Since

    Feb. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA6 asyn A30P

    Plasmid

    #105729

    Purpose

    Mammalian expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

    Insert

    alpha-synuclein A30P (SNCA Human)
    

    Expression

    Mammalian

    Mutation

    A30P

    Promoter

    CMV

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA3 Flag Jnk1a1(apf)

    Plasmid

    #13846

    Depositor

    Roger Davis

    Article

    Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

    Insert

    Jnk1a1(apf) (MAPK8 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    replaced dual phosporylation motif Thr(183)-Pro-T…

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET42b-HF-BE3

    Plasmid

    #87438

    Purpose

    Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

    Insert

    HF-BE3 (Apobec1 Human, Rat, Bacillus phage PBS2)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Topo_SpCas9.tracr_AsCas12a.DR

    Plasmid

    #155050

    Purpose

    TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
    

    Use

    CRISPR

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pXJ42-p200 CUX1

    Plasmid

    #100813

    Purpose

    Plasmid vector expressing human p200 CUX1 (amino acids 1-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag

    Depositor

    Alain Nepveu

    Article

    Wilson et al J Biol Chem. 2009 Jan 2;284(1):36-45. do

    Insert

    p200 CUX1 (CUX1 Human)
    

    Use

    Note that this plasmid does not have an sv40 ori

    Tags

    HA and Myc

    Expression

    Mammalian

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HRAS

    Plasmid

    #55653

    Purpose

    expression of mouse HRAS

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    HRAS (Hras Mouse)
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMT-sox10-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80062

    Purpose

    Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    Sox10 promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CAG-DIO-ChroME2f-ST-P2A-H2B-mRuby3

    Plasmid

    #171150

    Purpose

    Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2f-P2A-H2B-mRuby3
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge