We narrowed to 1,046 results for: Psp;

1. pSpCas9(BB)-2A-mCherry

   Plasmid

   #197420

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9_T2A_mCherry sgBFP

   Plasmid

   #192929

   Purpose

   All-in-one plasmid for cutting BFP region in DSB Spectrum V2

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A mCherry

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSP64T (DM#111)

   Plasmid

   #15030

   Depositor

   Douglas Melton

   Article

   Krieg et al Nucleic Acids Res. 1984 Sep 25. 12(18):7

   Insert

   beta-globin 5' and 3' UTR
   

   Expression

   Bacterial

   Available Since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. dPspCas13b-mutFTO

   Plasmid

   #223697

   Purpose

   Expressing fusion protein of dPspCas13b and inactive mutant of human FTO

   Depositor

   Chuan He

   Article

   Wei et al Science. 2022 May 27;376(6596):968-973.

   Insert

   dPspCas13b-GGS-mutFTO (FTO Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSP6-RNAP

   Plasmid

   #48143

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   rna polymerase SP6
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   V314A (numbering based on NCBI Sequence ID: NP_85…

   Available Since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEC2483 (pSpy1C)

   Plasmid

   #220248

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpyC-GFP

   Plasmid

   #220191

   Purpose

   sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   pBG42 promoter with GFP in sgRNA site
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   monomeric superfolder green fluorescent protein

   Promoter

   pBG42

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9(BB)-2A-miRFP670

   Plasmid

   #91854

   Purpose

   Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988

   Depositor

   Ralf Kuehn

   Inserts

   hSpCas9-2A-miRFP670
   BB-guide RNA
   

   Use

   CRISPR

   Tags

   2A-miRFP670 and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh and U6

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3-HA PSPC1

   Plasmid

   #101764

   Purpose

   Expresses PSPC1 in mammalian cells

   Depositor

   Yuh-Shan Jou

   Article

   Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

   Insert

   paraspeckle component 1 (PSPC1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSP64-mHCN2

    Plasmid

    #53060

    Purpose

    Expresses alpha subunit of mouse HCN2 channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Chen et al J Biol Chem. 2000 Nov 17;275(46):36465-7

    Insert

    Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (Hcn2 Mouse)
    

    Use

    Xenopus oocyte expression

    Mutation

    E55G, R237H, and K283R polymorphisms compared to …

    Promoter

    SP6

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSplit2-NO-PRPF6

    Plasmid

    #51740

    Purpose

    Express LucN-PRPF6 fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    PRP6 (PRPF6 Human)
    

    Tags

    FLAG and Luciferase (1-398aa)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpyB-GFP

    Plasmid

    #220190

    Purpose

    sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG35 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG35

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSP64-hKCNE1

    Plasmid

    #53049

    Purpose

    Expresses beta subunit for IKs channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

    Insert

    Potassium voltage-gated channel subfamily E member 1 (KCNE1 Human)
    

    Use

    Xenopus oocyte expression

    Promoter

    SP6

    Available Since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. BII-CPspaH2B

    Plasmid

    #133385

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pscALPSpuro-RsACE2

    Plasmid

    #159665

    Purpose

    Expresses Rhinolophus sinicus ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    rhinolophus sinicus ACE2 (ACE2 rhinolophus sinicus)
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-PSPC1-CT

    Plasmid

    #124374

    Purpose

    expresses EGFP-PSPC1-CT131 in mammalian cell

    Depositor

    Yuh-Shan Jou

    Article

    Lang et al Nat Commun. 2019 Dec 16;10(1):5716. doi:

    Insert

    PSPC1 CT (PSPC1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSPORT-RREB1

    Plasmid

    #41145

    Depositor

    Joshua Mendell

    Article

    Kent et al Oncogene. 2012 Jul 2. doi: 10.1038/onc.2

    Insert

    RREB-1 (RREB1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSP64TS (DM#117)

    Plasmid

    #15034

    Depositor

    Douglas Melton

    Article

    Krieg et al Nucleic Acids Res. 1984 Sep 25. 12(18):7

    Insert

    beta-globin 5' and 3' UTR
    

    Expression

    Bacterial

    Available Since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pSP64-hERG1a

    Plasmid

    #53051

    Purpose

    Expresses alpha subunit of hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Promoter

    SP6

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSPL3-ABCC8-2

    Plasmid

    #135916

    Purpose

    Encodes ABCC8 exon 2 wild type for the analysis of splicing variants

    Depositor

    Diana Valverde Pérez

    Article

    Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

    Insert

    ATP-binding Cassette subfamily C member 8 (ABCC8 Human)
    

    Use

    Exon trapping

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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