We narrowed to 7,726 results for: PRS;

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pRS416-PTEF1-MCP-5M-ECM33_intron

Plasmid

#127600

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-5P-ECM33_intron

Plasmid

#127599

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins inserted near 5′ end of intron

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-ECM33_intron

Plasmid

#127598

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS-ATG1(mut)(406)

Plasmid

#122069

Purpose

Expressing a mutant ATG1

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Mutation

Codon changes without changing the amino acid seq…

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-ATG7p-ATG1(406)

Plasmid

#122071

Purpose

Expressing ATG1

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Mutation

The native promoter was replaced by ATG7 promoter…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG9-GFP T119D

Plasmid

#114668

Purpose

Expresses yeast ATG9 (T119D) under the endogenous promoter with a GFP C-terminal tag

Depositor

Daniel Klionsky

Article

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Promoter

ATG9

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 N295D/D450G

Plasmid

#87287

Purpose

Encodes Hsh155 N295D D450G for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

N295D & D450G

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 R294L/H331D

Plasmid

#87282

Purpose

Encodes Hsh155 R294L H331D for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

R294L & H331D

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 N295D/H331D

Plasmid

#87285

Purpose

Encodes Hsh155 N295D H331D for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

N295D & H331D

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 N295D/K335E

Plasmid

#87286

Purpose

Encodes Hsh155 N295D K335E for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

N295D & K335E

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 R294L/D450G

Plasmid

#87284

Purpose

Encodes Hsh155 R294L D450G for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

R294L & D450G

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 R294L/K335E

Plasmid

#87283

Purpose

Encodes Hsh155 R294L K335E for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

R294L & K335E

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1deltaC(E224Q, GiVL-2)

Plasmid

#32035

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Tags

His6-SUMO

Expression

Bacterial

Mutation

E224Q and N195-M215 replaced by P697-D714 of GiDi…

Promoter

T7

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1deltaC(E147Q/D151N)

Plasmid

#32024

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Tags

His6-SUMO

Expression

Bacterial

Mutation

E147Q/D151N

Promoter

T7

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal-RNQ1 L94A

Plasmid

#18691

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 L94A

Expression

Yeast

Mutation

Leucine 94 mutated to Alanine (L94A)

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
7861 pRS305-pUG72-HSC82out

Plasmid

#10899

Depositor

Susan Lindquist

Article

Cowen et al Science. 2005 Sep 30. 309(5744):2185-9.

Insert

HSC82 URA3 (HSC82 Budding Yeast)

Use

Cre/Lox

Expression

Yeast

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only

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SepRS(2)/pSertRNA(B4)/EF-Sep

Plasmid

#173897

Purpose

The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

Depositor

Jason W Chin

Article

Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

Inserts

SepRS(2)
pSertRNA
EF-Sep

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp15-mNeongreen

Plasmid

#182389

Purpose

Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET his-eGFP

Plasmid

#113551

Purpose

For bacterial expression and purification of eGFP

Depositor

Jeanne Stachowiak

Article

Busch et al Nat Commun. 2015 Jul 24;6:7875. doi: 10.

Insert

his-eGFP

Expression

Bacterial

Mutation

A206K

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-mSA-EGFP

Plasmid

#39862

Depositor

Sheldon Park

Article

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin fused to EGFP

Tags

6xHis, EGFP, and FLAG

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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