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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,033 results for: LEA

Showing: 351 - 400 of 16033 results

  2. Cerulean-Venus-FRET-10

    Plasmid
    #58177
    Purpose
    Localization: Cytosolic FRET, Excitation: , Emission:
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Cerulean and Venus
    Expression
    Mammalian
    Available Since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. PEA1-Nuclease-GFP

    Plasmid
    #171994
    Purpose
    Delivers all prime editing nuclease components in a single, GFP selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    171994-plasmid-map-sequence-id-338231

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  6. mCerulean3-mVenus-FRET-10

    Plasmid
    #58180
    Purpose
    Localization: Cytosolic FRET, Excitation: , Emission:
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mCerulean3 and mVenus
    Expression
    Mammalian
    Available Since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pCS2+ H2B-mCerulean

    Plasmid
    #99268
    Purpose
    Contains a fusion of human histone H2B to fluorescent protein mCerulean in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription
    Depositor
    Philipp Keller
    Insert
    mCerulean-3XFLAG
    Tags
    H2B
    Expression
    Mammalian
    Promoter
    SP6
    Available Since
    Sept. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    99268-plasmid-map-sequence-id-192965

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  13. pOpen-Endonuclease IV E. coli

    Plasmid
    #165551
    Purpose
    DNA AP endonuclease; Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini; Also has 3'-diesterase activity which can remove 3' phosphate, 3'-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Endonuclease IV E. coli
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165551-plasmid-map-sequence-id-339868

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  14. mCerulean-Gamma-Tubulin-17

    Plasmid
    #55370
    Purpose
    Localization: Centrosomes, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    Gamma-Tubulin (TUBG1 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. mCerulean-PCNA-19-SV40NLS-4

    Plasmid
    #55386
    Purpose
    Localization: Nucleus, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    3xSV40 NLS and mCerulean
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pCerulean-N1 Cyclin B1 (1147)

    Plasmid
    #61852
    Purpose
    mammalian expression of Cyclin B1 fused to Cerulean
    Depositor
    Jonathon Pines
    Insert
    Cyclin B1 (CCNB1 Human)
    Tags
    Cerulean
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  21. mCerulean-Beta-Catenin-20

    Plasmid
    #55352
    Purpose
    Localization: Tight Junctions, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    Beta-Catenin (Ctnnb1 Mouse)
    Tags
    mCerulean
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    55352-plasmid-map-sequence-id-177099

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  34. mCerulean-proα2(I)-G610C

    Plasmid
    #119837
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-5 replaced by fluorescent tag, Gly…
    Promoter
    CMV
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    119837-plasmid-map-sequence-id-233867

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  35. G protein alpha-s-mCerulean

    Plasmid
    #66084
    Purpose
    G protein alpha-s internally tagged with mCerulean and EE epitope
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Insert
    G-alpha-s-EE-mCerulean (Gnas Aequorea victoria, Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available Since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    66084-plasmid-map-sequence-id-118926

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  36. mCerulean-cpVenus-Metallothionein-3

    Plasmid
    #58176
    Purpose
    Localization: Cytosolic FRET, Excitation: , Emission:
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    cpVenus-Metallothionein and mCerulean
    Expression
    Mammalian
    Available Since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  47. pAS1-his-24::Cerulean

    Plasmid
    #124323
    Purpose
    Expressing Nuclear Localized Cerulean
    Depositor
    Andrew Leifer
    Insert
    Cerulean (his-24 Nematode)
    Expression
    Worm
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    124323-plasmid-map-sequence-id-241693

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  48. mCerulean-Alpha Actinin-19

    Plasmid
    #55351
    Purpose
    Localization: Alpha Actinin, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    Alpha-Actinin (ACTN1 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 351 - 400 of 16033 results