We narrowed to 4,818 results for: U6

1. pAV-U6+27-Tornado-biSiRA

   Plasmid

   #196345

   Purpose

   Overexpression of circular biSiRA

   Depositor

   Murat Sunbul

   Article

   Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

   Insert

   Tornado-biSiRA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-U6-sgRNA-hSyn-mCherry

   Plasmid

   #87916

   Purpose

   sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

   Depositor

   Alex Hewitt

   Article

   Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC103-PBNeo-U6-sgRNA-Expression

   Plasmid

   #48228

   Purpose

   sgRNA expression on PiggyBac vector with Neo-selectable marker

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. U6-5'+3' MS2-CircRNA

   Plasmid

   #213752

   Purpose

   For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. v1em-Cterm-PE2max-U6-pegRNA

   Plasmid

   #198733

   Purpose

   AAV genome encoding C-terminal PE2max and U6 expression cassette

   Depositor

   David Liu

   Article

   Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

   Insert

   NpuC-CtermPE2max
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. v1em-Nterm-PE2max-U6-sgRNA

   Plasmid

   #198732

   Purpose

   AAV genome encoding N-terminal PE2max and U6 expression cassette

   Depositor

   David Liu

   Article

   Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

   Insert

   Nterm PE2max-NpuN
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-U6/sgClta-sfGFP-Clta

   Plasmid

   #200261

   Purpose

   U6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.

   Depositor

   Justin Eyquem

   Article

   Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

   Insert

   sfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT4-U6-sgRNA-CMV-EGFP

   Plasmid

   #239587

   Purpose

   Sleeping-beauty based EV for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

   Depositor

   Andreas Moor

   Article

   Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

   Type

   Empty backbone

   Use

   Sleeping beauty transposone

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-FLEXfrt-SaCas9-U6-sgRNA

   Plasmid

   #124845

   Purpose

   Vector for FLP-dependent expression of SaCas9 with gRNA

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   SauCas9 gRNA scaffold
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAV-U6+27-Tornado-Squash

    Plasmid

    #177915

    Purpose

    Expresses Squash aptamer as circular RNA in mammalian cells using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Tornado-Squash
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. U6-AsCas12f-full-length-sgRNA

    Plasmid

    #204638

    Purpose

    Expression of full-length AsCas12f sgRNA in mammalian cells

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    full-length AsCas12f sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. U6-sgH11-eCas9-T2A-BlastR

    Plasmid

    #172492

    Purpose

    For CRISPR-assisted HDR, Hipp11 locus

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Insert

    , BlastR and sgRNA targeting the Hipp11 (H11) safe harbor locus
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ISDra2-TnpB-mCherry-U6-ωRNA*

    Plasmid

    #212967

    Purpose

    Expression vector for expression of ISDra2-TnpB driven by chicken β-actin promoter and mCherry driven by CMV promoter

    Depositor

    Chunlong Xu

    Article

    Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

    Inserts

    ISDra2-TnpB-T2A-mCherry
    ωRNA*
    

    Use

    CRISPR

    Tags

    NLS and NLS-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG, T7 and U6

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC57kan-T7-gRNA-U6 V2

    Plasmid

    #115520

    Purpose

    To construct multiple sgRNA expression vector for CRISPR

    Depositor

    Xingxu Huang

    Insert

    sgRNA scaffolf-U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO5.U6.DR130(CasRX)_Empty.EFS.tRFP657

    Plasmid

    #212961

    Purpose

    Lentiviral plasmid for the cloning of mature gRNAs of the RfxCas13d (CasRx) CRISPR system. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

    Depositor

    Roland Rad

    Article

    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

    Insert

    DR1_CasRx_backbone
    

    Use

    CRISPR and Lentiviral

    Tags

    TagRFP657

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-FLEX-SaCas9-U6-sgGrin1

    Plasmid

    #124852

    Purpose

    Mutagenesis of Grin1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin1 (Grin1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. U6-BbsI-GGsite-mCherry-Puro

    Plasmid

    #221232

    Purpose

    Cloning vector for sgRNA with mCherry and Puro

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-FLEX-SaCas9-U6-sgSlc18a2

    Plasmid

    #124849

    Purpose

    Mutagenesis of Slc18a2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-FLEXfrt-SaCas9-U6-sgSlc18a2

    Plasmid

    #124862

    Purpose

    Mutagenesis of Slc18a2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-SpCas9-GFP-U6-gRNA

    Plasmid

    #79144

    Purpose

    All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC

    Depositor

    Jizhong Zou

    Article

    Stoehr et al JCI Insight. 2019 May 21;5. pii: 128496.

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-FLEX-SaCas9-U6-sgSlc17a8

    Plasmid

    #124848

    Purpose

    Mutagenesis of Slc17a8

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a8 (Slc17a8 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PB-hEF1A-MG-U6-sgRNA

    Plasmid

    #175505

    Purpose

    All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a, U6

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV.minBG-EGFP-W3SL_2x-U6-sasgAi14

    Plasmid

    #231365

    Purpose

    minBG-driven EGFP, also encoding U6-driven sgRNAs to direct saCas9 to both sides of stop cassette in Ai14 mice.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-U6-sasgRNACMV- mCherry-WPREpA

    Plasmid

    #217015

    Purpose

    S. aureus sgRNAs backbone along with mCherry expression

    Depositor

    Nadav Ahituv

    Article

    Matharu et al Science. 2018 Dec 13. pii: science.aau06

    Insert

    mCherry
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-FLEX-SaCas9-U6-sgGabrg2

    Plasmid

    #124857

    Purpose

    Mutagenesis of Gabrg2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg2 (Gabrg2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. U6-hGRIN2B-CAG-ps-SpCas9

    Plasmid

    #102851

    Purpose

    A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    3X Flag and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHR-U6-sgRNA-CMV-puro

    Plasmid

    #169450

    Purpose

    backbone for sgRNA expression

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. U6-(BbsI)sgRNA_CAG-Venus-bpA

    Plasmid

    #86985

    Purpose

    For cloning and expression of sgRNA together with Venus expression

    Depositor

    Ralf Kuehn

    Article

    Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

    Insert

    Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Venus GFP variant

    Promoter

    CAG

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCFD5-U6-3-t-attP40

    Plasmid

    #133561

    Purpose

    gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    attP40 region guide RNAs
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLb-Cpf1-pGL3-U6-sgRNA

    Plasmid

    #107682

    Purpose

    Expresses LbCpf1 crRNA in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge