We narrowed to 4,486 results for: LTA

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NmCitrine-PHdomain

Plasmid

#66783

Purpose

Expression of PIP2 binding protein in sea urchin embryos

Depositor

Amro Hamdoun

Article

Campanale et al Dev Dyn. 2014 Jul;243(7):917-27. doi: 10

Insert

PLCdelta (PLCD1 Human)

Use

Sea urchin, zebrafish, xenopus

Tags

mCitrine

Expression

Mammalian

Mutation

only contains PH domain

Promoter

SP6

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
pLKO.1 shPIK3CD.v1 puro

Plasmid

#58706

Purpose

Lentiviral shRNA vector for knockdown of human PIK3CD

Depositor

Kevin Janes

Article

Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

Insert

shPIK3CD (PIK3CD Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only
GAL FLAG25QDProGFP p306

Plasmid

#15831

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q Delta Pro (HTT Human)

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
GAL FLAG103QDProGFP p306

Plasmid

#15834

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 103Q Delta Pro (HTT Human)

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
CAG-Cremyc-2A-GFP

Plasmid

#116879

Purpose

Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

Depositor

Qiang Lu

Article

Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

Insert

Cremyc-2A-GFP

Expression

Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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Yellow Cameleon-Nano15/pBig

Plasmid

#51962

Purpose

Expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in Dictyostelium

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano15

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-PlcR(K30/32L)

Plasmid

#181831

Purpose

Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR (K30L,K32L)

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-syn-ChR2-Kv-P2A-H2b-mRuby2

Plasmid

#89570

Purpose

Channelrhodopsin localized to neuronal soma with simultaneous nuclear labeling

Depositor

McLean Bolton

Article

Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

Inserts

Channelrhodopsin
Histone 2B

Use

AAV

Tags

mRuby2

Expression

Mammalian

Mutation

H134R

Promoter

synapsin

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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CaV2.1 (pCR3.1)

Plasmid

#26573

Depositor

Diane Lipscombe

Article

Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

Insert

Cacna1a (Cacna1a Mouse)

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
CVS-N2c-nl.EGFP-SypGFP

Plasmid

#172380

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

nuclear-localized EGFP + SypEGFP

Use

Neurotropic virus

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Klf4

Plasmid

#19764

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

Insert

Klf4 (Klf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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GAL FLAG72QDProGFP p306

Plasmid

#15833

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 72Q Delta Pro (HTT Human)

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
His_Naa80d in pTYB12

Plasmid

#188455

Purpose

Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

Naa80 (NAA80 Human)

Tags

intein-CBD, 6xHis

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-E2_Crimson

Plasmid

#172377

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

E2_Crimson

Use

Neurotropic virus

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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EYFP-Clathrin

Plasmid

#20921

Depositor

Xiaowei Zhuang

Article

Rust et al Nat Struct Mol Biol. 2004 Jun . 11(6):56

Insert

Clathrin, light polypeptide (Lca) (Clta Mouse)

Tags

EYFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pMTG06-Amp-FRT

Plasmid

#128275

Purpose

Deg-LITer2.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-RRRG-P2A-GFP

Depositor

Gabor Balazsi

Article

Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Inserts

TetR-LOV-RRRG
EGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pBS NL4-3 envFS eGFP Δrev

Plasmid

#101348

Purpose

All mutations in rev are silent with respect to the tat reading frame. Start ATG->ACG and nts 68-71 were mutated AGC->TCA to change tyrosine to a stop.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Mutation

delta rev

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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LZF1826 pAAV_hSyn-DiO-SomArchon-EGFP-P2A-stGtACR2

Plasmid

#135412

Purpose

simultaneous optical recording and manipulation of electrical activity in neurons

Depositor

Adam Cohen

Article

Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

Insert

SomArchon-EGFP-P2A-stGtACR2

Use

AAV

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pGem-nHyPer2:sHyper2

Plasmid

#84737

Purpose

Simultaneous expresion of nuclear and stromal HyPer2 in plant cells

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

HyPer2 targeted to nucleus

Tags

NLS-SV40

Expression

Plant

Promoter

pFMV/p35S

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC101 NL4-3 envFS Δrev ΔRRE RTEm26CTE

Plasmid

#101351

Purpose

HIV-1 delta Rev was cloned into pSC101 and modified to include an RTEm26CTE element in order to utilize the NXF1 RNA export pathway. The RRE was also mutated.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBSc + MShal

Plasmid

#16166

Depositor

Larry Salkoff

Article

Pak et al Proc Natl Acad Sci U S A. 1991 May 15. 8

Insert

Mus musculus potassium voltage-gated channel (Kcnd1 Mouse)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pFUGW-ZipACR-EYFP

Plasmid

#88844

Purpose

Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

ZipACR-NotI-EYFP

Use

Lentiviral

Tags

EYFP

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pHW450

Plasmid

#198831

Purpose

Voltage-sensitive fluorescent protein.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::VSFP-Butterfly-1-2::let-858 3'UTR

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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GAL FLAG25QDProCFPp303

Plasmid

#15584

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q Delta Pro (HTT Human)

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
GAL FLAG46QDProGFP p306

Plasmid

#15832

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 46Q Delta Pro (HTT Human)

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
pTRCHisB-TRF2∆M

Plasmid

#50489

Purpose

E. Coli N-term HexHis Tagged Protein Expression Vector

Depositor

Jack Griffith

Article

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TRF2 Delta Myb (TERF2 Human)

Tags

6x His, T7 tag, and TEV protease site

Expression

Bacterial

Mutation

Contains amino acids 3-437

Promoter

pTrc

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
CVS-N2c-tdTomato-ChIEF

Plasmid

#172370

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

tdTomato + ChIEF

Use

Neurotropic virus

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUGW-RapACR-EYFP

Plasmid

#111075

Purpose

Fast anion channelrhodopsin (current half-decay time 3-10 ms depending on voltage and light intensity) for time-resolved inhibition of neuronal spiking

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin RapACR

Use

Lentiviral

Tags

EYFP

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CVS-N2c-EYFP

Plasmid

#172383

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

EYFP

Use

Neurotropic virus

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CVS-N2c-mCitrine

Plasmid

#172384

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

mCitrine

Use

Neurotropic virus

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CVS-N2c-EGFP-ChIEF

Plasmid

#172371

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

EGFP + ChIEF

Use

Neurotropic virus

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CVS-N2c-SypRFP

Plasmid

#172381

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

Synaptophysin1-RFP fusion protein

Use

Neurotropic virus

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CVS-N2c-jGCaMP8f

Plasmid

#172387

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

GCaMP8f

Use

Neurotropic virus

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CVS-N2c-nl.EGFP-tdTomato

Plasmid

#172382

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

nuclear-localized EGFP + tdTomato

Use

Neurotropic virus

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Myog'-2A-mCherryNLS-PGK-Purodtk

Plasmid

#69547

Purpose

Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

Depositor

Charles Gersbach

Inserts

2A-mCherry-NLS
Myogenin 5' homology arm (Myog Mouse)
Myogenin 3' homology arm (Myog Mouse)
Puro-delta-tk

Use

Mouse Targeting

Tags

2x nuclear localization signal and PVT1-2A "…

Mutation

Silent V205V mutation in myogenin gene to prevent…

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
y2

Plasmid

#53586

Purpose

Produces an active Arabidopsis thaliana lycopene epsilon-cyclase in E. coli. Use with pAC-LYC or pAC-LYCipi to make delta-carotene in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 1996 Sep;8(9):1613-26.

Insert

lcyE (LUT2 Mustard Weed)

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR4_FLAG/HA_FUS_His6

Plasmid

#26365

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

FUS (FUS Human)

Use

Gateway Cloning

Tags

FLAG/HA and His6

Mutation

delta 527 (stop codon)

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
Hiv7CMV-Cremyc-2A-GFP

Plasmid

#117148

Purpose

Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

Depositor

Qiang Lu

Article

Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

Insert

Cremyc-2A-GFP

Use

Lentiviral

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GAL FLAG46QDProCFPp303

Plasmid

#15585

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 46Q Delta Pro (HTT Human)

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
GPD 25QDProGFP p416

Plasmid

#15568

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q Delta Pro (HTT Human)

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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