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Plasmid#128195PurposeLevel1 ZmUBIp::A3A-PBE (wheat codon optimized):NOStDepositorInsertZmUBIp::A3A-PBE (wheat codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH68
Plasmid#128188PurposeLevel1 ZmUBIp::SpCas9-NG (human codon optimized):NOStDepositorInsertZmUBIp::SpCas9-NG (human codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH82
Plasmid#128193PurposeLevel1 ZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOStDepositorInsertZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH93
Plasmid#128196PurposeLevel1 ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOStDepositorInsertZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH59
Plasmid#128185PurposeLevel1 ZmUBIp::StCas9 (Arabidopsis codon optimized):NOStDepositorInsertZmUBIp::StCas9 (Arabidopsis codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH81
Plasmid#128192PurposeLevel1 ZmUBIp::ScCas9 (wheat codon optimized):NOStDepositorInsertZmUBIp::ScCas9 (wheat codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)
Plasmid#174138PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarletDepositorInserthuman codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationVRER=D1135V/G1218R/R1335E/T1337RPromoterCMV and T7Available sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)
Plasmid#174139PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarletDepositorInserthuman codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationVRQR=D1135V/G1218R/R1335Q/T1337RPromoterCMV and T7Available sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFH132
Plasmid#136547PurposeLevel1 ZmUBIp::FnCas12a (human codon optimized):NOStDepositorInsertZmUBIp::FnCas12a (human codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH133
Plasmid#136548PurposeLevel1 ZmUBIp::LbCas12a (human codon optimized):NOStDepositorInsertZmUBIp::LbCas12a (human codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH25
Plasmid#131764PurposeLevel0 SpCas9, wheat codon optimized (version 3)DepositorInsertSpCas9, wheat codon optimized, version 3
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH109
Plasmid#128197PurposeLevel1 ZmUBIp::nScCas9 (wheat codon optimized):NOStDepositorInsertZmUBIp::nScCas9 (wheat codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH60
Plasmid#128186PurposeLevel1 ZmUBIp::FnCas12a (rice codon optimized):NOStDepositorInsertZmUBIp::FnCas12a (rice codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH61
Plasmid#128187PurposeLevel1 ZmUBIp::LbCas12a (rice codon optimized):NOStDepositorInsertZmUBIp::LbCas12a (rice codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH70
Plasmid#128190PurposeLevel1 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOStDepositorInsertZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH77
Plasmid#125816PurposeLevel0 enCas9-PolI3M-TBD, wheat codon optimizedDepositorInsertenCas9-PolI3M-TBD, wheat codon optimized
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH91
Plasmid#125819PurposeLevel0 nScCas9 (nickase), wheat codon optimizedDepositorInsertnScCas9 (nickase), wheat codon optimized
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
R777-E237 Hs.RASAL3
Plasmid#70521PurposeGateway ORF clone of codon optimized human RASAL3 [NM_022904.1] with stop codon (for native or N-terminal fusions)DepositorInsertRASAL3 (RASAL3 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTDpelB-C_sfYFPTwinStrep
Plasmid#45944Purposeexpresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequenceDepositorInsertsynthetic sfYFP (codon usage adapted to P.putida KT2440)
UseTagsPelB export sequence and Twin-Strep-tagExpressionBacterialMutationPromoterlacIq/PtrcAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-V5.hOr47a
Plasmid#126474PurposeHuman codon-optimized version of D. melanogaster Or47a (hOr47a) without N-terminal tags for improved intracellular trafficking in mammalian cellsDepositorInsertOr47a (Or47a Fly)
UseTagsV5 tagExpressionMammalianMutationcodon optimization for H. sapiensPromoterCMVAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only