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Plasmid#60161PurposeYeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.DepositorInsertyEVenus-PEST
UseTagsPEST degronExpressionYeastMutationPromoterSIC1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB784
Plasmid#60163PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB655
Plasmid#60142PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationA4VPromoterMET17prAvailable sinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ158
Plasmid#69307PurposeGateway entry vector with hSpCas9 (Human codon optimized)DepositorInserthSpCas9 (Human codon optimized)
UseCRISPRTags3XFLAGExpressionMutationNLS fusionPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
B3rec.zf1
Plasmid#108354PurposeZebrafish codon optimized B3 recombinase that acts on blown-out sitesDepositorInsertB3 recombinase
UseSynthetic BiologyTagsExpressionMutationZebrafish codon optimizedPromoterlacAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNB788
Plasmid#60165PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUB_NonoptλHAramp-FLuc100
Plasmid#210579PurposeExpresses Nonoptimal lambda HA-Optimal Firefly luciferaseDepositorInsertNonoptimal lambda HA-Optimal Firefly luciferase
UseLentiviral and LuciferaseTagslambda HAExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SM-FLuc100-pA
Plasmid#210584PurposeExpresses SM(FLAG)-optimal Firefly luciferase - pADepositorInsertSpaghetti monster (FLAG)-optimal FLuc
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNB786
Plasmid#60151PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB812
Plasmid#60160PurposeYeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.DepositorInsertyEVenus-PEST
UseTagsPEST degronExpressionYeastMutationPromoterRNR1prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
469_pME_iCre
Plasmid#171792PurposepME-Vector with codon improved iCre sequence. Tol2-kit compatible.DepositorInsertcodon-optimized iCre sequence
UseTagsExpressionMutationPromoterNoneAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-Opt WWP1
Plasmid#179327PurposeE. coli optimized expression of GST-fusion of human WWP1DepositorInsertWWP1 (WWP1 Human)
UseTagsGSTExpressionBacterialMutationCodon optimized for expression in E. coliPromoterAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-WWP1F792D
Plasmid#179328PurposeE. coli optimized expression of GST-fusion of human WWP1 F792DDepositorInsertWWP1 F792D (WWP1 Human)
UseTagsGSTExpressionBacterialMutationF792D mutation; Codon Optimized for expression in…PromoterAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)
Plasmid#182287PurposeEncodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
UseTagsnuclear export signal (NES)ExpressionMammalianMutationY306A/Y384F (AF) double mutant of Methanosarcina …PromoterCMV and U6Available sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF-5xT7-TtCsm
Plasmid#128572PurposeFor expression of T. thermophilus Csm in E. coli (Csm1-5)DepositorInsertsCsm1
Csm2, Csm3, Csm4, Csm5
UseTagsHRV 3C protease cleavage site and 10xHis tag on C…ExpressionBacterialMutationCodon optimized for expression in E. coli and Cod…PromoterT7 promoter, lac operatorAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mitoTPNOX
Plasmid#87854PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertmitoTPNOX
UseTagsC-terminal FLAG tag with a linker and mitochondri…ExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMLM3705
Plasmid#47754PurposeExpresses mammalian cell codon-optimized dCas9-VP64DepositorInsertcodon optimized dCas9-VP64
UseCRISPRTags3x FLAG, NLS, and VP64ExpressionMammalianMutationD10A and H840A mutations in Cas9 (catalytically i…PromoterCMVAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2+/N-Halo
Plasmid#184417Purposevector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesisDepositorTypeEmpty backboneUseFor mrna synthesisTagsHalo-tagExpressionMutationCodon optimized for expression in Xenopus laevis …PromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHsp70-Cas9
Plasmid#45945PurposeThe codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.DepositorInsertCodon optimized Cas9
UseCRISPRTags3x FLAGExpressionMutationSilent change from C to A at bp 834 of insertPromoterAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-1
Plasmid#61736PurposeStreptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNADepositorInsertssSpCas9
tracrRNA
crRNA cassette
UseCRISPRTagsExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsIAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only