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Addgene
Showing: 81 - 100 of 217 results
  1. C1-mCitrine-FKBP-EZRabd(t567D)

    Plasmid
    #155227
    Purpose
    Acute increase in MPA when used with FRB-FKBP dimerizaton system upon addition with Rapamycin
    Depositor
    Insert
    mCitrine fused to FKBP and the actin BD of Ezrin (T567D), aa552-586 for use with FRB and rapamycin induced dimerization system
    Use
    Tags
    mCitrine
    Expression
    Mammalian
    Mutation
    Changed Thr 567 to Asp in original Ezrin
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. str-KDEL_Stx5LdeltaER-SBP-mCitrine

    Plasmid
    #154846
    Purpose
    Synchronize trafficking of Stx5LdeltaER (M55V) from the ER, for use with FLIM (RUSH system)
    Insert
    Stx5 (Long isoform) (STX5 Human)
    Use
    Tags
    Streptavidin Binding Protein (SBP) and mCitrine
    Expression
    Mammalian
    Mutation
    Changed Methionine 55 to Valine, causes loss of S…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  3. pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50463
    Purpose
    Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Availability
    Academic Institutions and Nonprofits only
  4. pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50464
    Purpose
    Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promote
    Depositor
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    Promoter
    human Synapsin 1
    Availability
    Academic Institutions and Nonprofits only
  5. pAAV-CaMKIIa-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50466
    Purpose
    Gq-coupled hM3D-IRES-mCitrine under the control of CaMKIIa promoter
    Depositor
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Availability
    Academic Institutions and Nonprofits only
  6. pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50467
    Purpose
    Gi-coupled hM4D-IRES-mCitrine under the control of CaMKIIa promoter
    Depositor
    Has Service
    AAV2 and AAV8
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Availability
    Academic Institutions and Nonprofits only
  7. pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid
    #50468
    Purpose
    Gs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoter
    Depositor
    Insert
    rM3D-IRES-mCitrine
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Availability
    Academic Institutions and Nonprofits only
  8. pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50470
    Purpose
    Gq-coupled hM3D-IRES-mCitrine under the control of GFAP promoter
    Depositor
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Availability
    Academic Institutions and Nonprofits only
  9. pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50471
    Purpose
    Gi-coupled hM4D-IRES-mCitrine under the control of GFAP promoter
    Depositor
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Availability
    Academic Institutions and Nonprofits only
  10. pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid
    #50472
    Purpose
    Gs-coupled rM3D-IRES-mCitrine under the control of GFAP promoter
    Depositor
    Has Service
    AAV5
    Insert
    rM3D-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Availability
    Academic Institutions and Nonprofits only
  11. hSyn-FAS-DO-ReaChR-mCitrine-WPRE

    Plasmid
    #64035
    Purpose
    Expresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructs
    Depositor
    Insert
    hSyn-FAS-DO-ReaChR-mCitrine-WPRE
    Use
    AAV
    Tags
    ReaChR tagged with mCitrine
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Availability
    Academic Institutions and Nonprofits only
  12. pN_35S/mCitrine/P_UBQ10/Derlin1-mOrange2

    Plasmid
    #118000
    Purpose
    Co-expression of mCitrine under the control of the CaMV 35S promoter and Derlin1 fused to mOrange2 (an ER-targeting marker protein) under the control of the A. thaliana ubiquitin-10 promoter.
    Depositor
    Insert
    Derlin1-mOrange2
    Use
    Plant/bacteria binary vector
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Ubiquitin-10
    Availability
    Academic Institutions and Nonprofits only
Showing: 81 - 100 of 217 results