422 results

1. Ochre (rEcΔ2.ΔA.B3.tW*)

   Bacterial Strain

   #234622

   Purpose

   Genomically recoded bacteria for site-specific incorporation of two nonstandard amino acids into proteins

   Depositor

   Farren Isaacs

   Article

   Grome et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

   Bacterial Resistance

   Hygromycin

   Available Since

   May 16, 2025

   Availability

   Academic Institutions and Nonprofits only

2. RHO3

   Bacterial Strain

   #124700

   Purpose

   E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

   Depositor

   Herbert Schweizer

   Article

   Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

   Bacterial Resistance

   Dap (400 µg/ml)

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

3. EC1000

   Bacterial Strain

   #71852

   Purpose

   RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

   Depositor

   Jan Kok, Todd Klaenhammer

   Article

   Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

   Bacterial Resistance

   Kanamycin

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

4. Escherichia coli DH10B-MOB

   Bacterial Strain

   #229395

   Purpose

   Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Bacterial Resistance

   60 ug/ml of dap

   Species

   Escherichia coli

   Available Since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

5. V. natriegens NC7

   Bacterial Strain

   #215356

   Purpose

   Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

   Depositor

   Buz Barstow

   Article

   Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

   Bacterial Resistance

   None

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

6. ML14

   Bacterial Strain

   #61911

   Purpose

   C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. tyrA gene deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

   Bacterial Resistance

   None

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

7. B-95.ΔAΔfabR

   Bacterial Strain

   #197934

   Purpose

   BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

   Depositor

   Kensaku Sakamoto

   Article

   Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

   Bacterial Resistance

   None

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

8. C321.ΔA.exp

   Bacterial Strain

   #49018

   Purpose

   This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

   Depositor

   George Church

   Article

   Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

   Bacterial Resistance

   Bleocin (zeocin)

   Species

   Escherichia coli

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

9. MutaT7

   Bacterial Strain

   #156460

   Purpose

   An E. coli strain that expresses MutaT7 for mutagenizing genes downstream from a T7 promoter.

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

   Bacterial Resistance

   Streptomycin

   Species

   Rattus norvegicus

   Available Since

   Oct. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

10. HB101 endA::frt lambda pir

    Bacterial Strain

    #45472

    Depositor

    Michele Swanson

    Article

    Bryan et al Mol Microbiol. 2011 Apr;80(1):231-47. do

    Bacterial Resistance

    Streptomycin

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

11. C321.∆A T7RNAP ompT- rne- lon-

    Bacterial Strain

    #182778

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivated

    Depositor

    Michael Jewett

    Article

    Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

    Bacterial Resistance

    Spectinomycin

    Available Since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

12. bMS.346

    Bacterial Strain

    #220588

    Purpose

    Increase editing efficiency of retron and oligo recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Bacterial Resistance

    None

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

13. V. natriegens NC1

    Bacterial Strain

    #215355

    Purpose

    Vibrio natriegens with genomically integrated natural competence regulator, TfoX, with chloramphenicol resistance. For zero-capital molecular biology applications.

    Depositor

    Buz Barstow

    Article

    Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

    Bacterial Resistance

    None

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

14. rApoI

    Bacterial Strain

    #156459

    Purpose

    An E. coli strain that expresses rat APOBEC1. Used as a control strain for mutagenesis experiments with MutaT7.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Bacterial Resistance

    Streptomycin

    Species

    Rattus norvegicus

    Available Since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

15. drApo1–T7

    Bacterial Strain

    #156461

    Purpose

    An E. coli strain that expresses an inactive variant of MutaT7. Used as a control strain for mutagenesis experiments with MutaT7.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Bacterial Resistance

    Streptomycin

    Species

    Rattus norvegicus

    Available Since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

16. pRK24

    Bacterial Strain

    #51950

    Purpose

    broad-host range natural conjugative plasmid

    Depositor

    Farren Isaacs

    Article

    Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi

    Bacterial Resistance

    Tetracycline

    Available Since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

17. DH10B-M.Osp807II

    Bacterial Strain

    #114267

    Purpose

    The strain expresses M.Osp807II methylase and zeocin selection marker from the arsB locus.

    Depositor

    Christopher A. O'Callaghan

    Article

    Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

    Bacterial Resistance

    Bleocin (zeocin)

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

18. DH10B-M2.NmeMC58II

    Bacterial Strain

    #114268

    Purpose

    The strain expresses M2.NmeMC58II methylase and zeocin selection marker from the arsB locus.

    Depositor

    Christopher A. O'Callaghan

    Article

    Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

    Bacterial Resistance

    Bleocin (zeocin)

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

19. B-95.ΔA

    Bacterial Strain

    #197933

    Purpose

    BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

    Depositor

    Kensaku Sakamoto

    Article

    Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

    Bacterial Resistance

    None

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

20. Syn61Δ3(ev5)

    Bacterial Strain

    #174514

    Purpose

    Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Bacterial Resistance

    Streptomycin

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only