422 results

1. EcM2.1

   Bacterial Strain

   #64052

   Purpose

   Optimized strain for high-efficiency genome manipulations using CoS-MAGE and tolC cycling

   Depositor

   George Church

   Article

   Gregg et al Nucleic Acids Res. 2014 Apr;42(7):4779-9

   Bacterial Resistance

   Ampicillin

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

2. E. coli BW25113 ΔtnaA ΔtrpR

   Bacterial Strain

   #205015

   Purpose

   Used for the characterization of the TrpR1-PtrpO1 biosensor system with the deletion of tnaA gene and trpR gene (no antibiotics marker)

   Depositor

   Yajun Yan

   Article

   Gong et al ACS Synth Biol. 2022 Jun 17;11(6):2175-2

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available Since

   Aug. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

3. TB204 △metA

   Bacterial Strain

   #230039

   Purpose

   Fluorescently labelled (GFP) E. coli, auxotrophic for methionine

   Depositor

   Olga Schubert, Martin Ackermann

   Article

   Micali et al Proc Natl Acad Sci U S A. 2023 Nov 7;120

   Bacterial Resistance

   None

   Available Since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

4. MG1655GFP

   Bacterial Strain

   #109393

   Purpose

   MG1655 with a "capacity monitor" inserted in the lambda phage integration site.

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

   Bacterial Resistance

   Kanamycin

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

5. ∆cosσε ∆HG

   Bacterial Strain

   #197132

   Purpose

   Deficient in the packaging cos site and encoding rhamnose-inducible P4 delta and epsilon genes. It also has tail fiber (H) and its chaperone (G) deleted.

   Depositor

   Baojun Wang

   Article

   Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

   Bacterial Resistance

   Hygromycin

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

6. RF13

   Bacterial Strain

   #62078

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

   Bacterial Resistance

   None

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

7. C321.∆A T7RNAP ompT- rne-

   Bacterial Strain

   #182777

   Purpose

   Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivated

   Depositor

   Michael Jewett

   Article

   Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

   Bacterial Resistance

   Spectinomycin

   Available Since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

8. sAJM.1505

   Bacterial Strain

   #108253

   Purpose

   Marionette-Pro. Marionette cluster inserted in the direction of leading strand replication, between 3,720,027 and 3,721,644 in E. coli BL21

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Bacterial Resistance

   Chloramphenicol

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

9. C321.deltaA (Isaacs lab)

   Bacterial Strain

   #73581

   Purpose

   Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Bacterial Resistance

   Ampicillin

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

10. BL21 Rosetta2 ΔrecBCD GamS

    Bacterial Strain

    #176586

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol and ampicillin

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

11. DH10B-ALT

    Bacterial Strain

    #61151

    Purpose

    DH10B modified to constitutively express araC, lacI, and tetR, integrated at the attB site on the E Coli genome. This is DH10B-ALT-Tet with the tetracycline resistance cassette deleted.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Bacterial Resistance

    None

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

12. BL21 Rosetta2 ΔrecBCD

    Bacterial Strain

    #176583

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

13. SQ171/pCSacB/ptRNA100

    Bacterial Strain

    #155206

    Purpose

    SQ171 strain supported by pCSacB and ptRNA100 plasmids

    Depositor

    Alexander Mankin

    Article

    Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

    Bacterial Resistance

    Kanamycin

    Available Since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

14. DH5alpha::DddI

    Bacterial Strain

    #179631

    Purpose

    For expression of toxic DddA

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Bacterial Resistance

    Gentamicin

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

15. nuc5-

    Bacterial Strain

    #40804

    Depositor

    George Church

    Article

    Mosberg et al PLoS One. 2012;7(9):e44638. Epub 2012 Se

    Bacterial Resistance

    Ampicillin

    Available Since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

16. 39R861+

    Bacterial Strain

    #119737

    Purpose

    Six stable plasmids can be used as size standards

    Depositor

    Ruth Hall

    Article

    Moran et al Plasmid. 2019 Mar;102:6-9. doi: 10.1016/

    Bacterial Resistance

    None

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

17. RF11

    Bacterial Strain

    #61961

    Purpose

    C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. metA gene deleted

    Depositor

    Toshio Iwasaki, Robert Gennis

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

18. LC-E18

    Bacterial Strain

    #115924

    Purpose

    Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Bacterial Resistance

    None

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

19. BL21ΔCF

    Bacterial Strain

    #102265

    Purpose

    ompC, ompF double knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

20. RF12

    Bacterial Strain

    #62077

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. trpA trpB genes deleted.

    Depositor

    Toshio Iwasaki, Robert Gennis

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only