161 results
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Plasmid Kit#1000000225PurposeThe CRISPR/Cas9 Insertional Mutagenesis Protocol (CRIMP) and toolkit (CRIMPkit) is a universal and highly efficient targeted insertional mutagenesis system designed for use in zebrafish.DepositorAvailable SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only
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Minimal Antibiotic Resistance Platform (ARP)
Plasmid Kit#1000000143PurposeUsed for the general dereplication of antibiotics in natural product extracts. Plasmids constitutively express individual resistance genes that target the most commonly found antibiotics.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
UbiGate Components (set1)
Plasmid Kit#1000000144PurposeGenerate bacterial expression cassettes of up to six ORFs simultaneously; also contains ubiquitin, the E1 UBA1, an E2 collection, and a selection of E3s and their substrates for ubiquitination assaysDepositorAvailable SinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2 Gateway compatible toolbox for the study of the nervous system and neurodegenerative disease
Plasmid Kit#1000000087PurposeTol2 Zebrafish toolkit: 4 promoters, 6 fluorophores, MultiSite Gateway (Invitrogen) compatible and cloning sites. Production transgenic zebrafish for neurodegenerative disordersDepositorAvailable SinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
B. subtilis Single Gene Deletion Library--Kanamycin
Plasmid Kit#1000000115PurposeB. subtilis Single Gene Deletion Library - Kanamycin: Arrayed deletion library in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.DepositorAvailable SinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
The Expanded EasyClone-MarkerFree toolkit
Plasmid Kit#1000000190PurposeThe Expanded EasyClone-MarkerFree toolkit is a new set of 8 validated chromosomal loci for the stable integration of heterologous DNA into baker’s yeast Saccharomyces cerevisiae.DepositorAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Hamdoun lab FP fusion plasmids
Plasmid Kit#1000000033PurposeScreen fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed, e.g. zebrafish, sea urchin, frog (Xenopus), or worm (C. elegans)DepositorAvailable SinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
Endy/Shearwin pOSIP plasmid set
Plasmid Kit#1000000035Purpose11 Plasmids for use in clonetegration: method for decreasing time and effort needed for integration of DNA into prokaryotic chromosomes. Facilitates cloning, expression of difficult genetic elementsDepositorAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
EXPRESSYALI
Plasmid Kit#1000000245PurposeThe EXPRESSYALI Set is a complete kit for the introduction of genes into Yarrowia chromosomal loci. The kit includes six part plasmids, 12 marker-free integrative vectors, and 11 gRNA plasmids.DepositorAvailable SinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
SapTrap-SEC kit for C. elegans genome engineering
Plasmid Kit#1000000109PurposeBuild sgRNA and homologous repair templates that incorporate self-excising drug selection cassette (SEC), using SapTrap cloning method. Addition of AAG junction.DepositorAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Yeast GPCR-sensor Toolkit
Plasmid Kit#1000000157PurposeThe Yeast GPCR-sensor Toolkit includes 42 plasmids for constructing highly-tunable GPCR-based biosensors in yeast.DepositorAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
The Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit
Plasmid Kit#1000000210PurposeToolkit contains Cas9-gRNA vectors, integration plasmids, and organellar localization markers as a set of plasmids for Cas9-mediated integration of tagged genes and markers into the yeast genome.DepositorAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
B. subtilis CRISPRi Essential Gene Knockdown Library
Plasmid Kit#1000000116PurposeB. subtilis CRISPRi Essential Gene Knockdown Library: Arrayed library that uses CRISPRi to sterically hinder transcription and therefore knock down expression of essential genes.DepositorAvailable SinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
MetClo DNA Assembly Kit
Plasmid Kit#1000000153PurposeMetClo DNA Assembly Kit allows modular, hierarchical assembly of DNA fragments using IIS restriction enzyme. Includes 3 E.coli strains for BsaI, BpiI and LguI-based MetClo assembly.DepositorAvailable SinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Zinc Finger Consortium Expression Vector and Modular Assembly Kit
Plasmid Kit#1000000005PurposeCan be used to efficiently assemble zinc finger arrays via modular assembly (Sangamo, Barbas, and Toolgen). Also includes the bacterial two-hybrid (B2H) system used to screen arrays for activity.DepositorAvailable SinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
Yeast GoldenBraid Cloning System and Toolkit
Plasmid Kit#1000000138PurposeModular cloning system for generation of high expression transcriptional units; Integrates in yeast (S. cerevisiae) genome at two different loci supporting high and stable transgene expressionDepositorAvailable SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRISPRainbow Kit for Multiplex Labeling
Plasmid Kit#1000000079PurposeCRISPR/Cas9 to tag up to 7 different genomic locations. Unique labeling system allows scientists to track DNA movement in live cells and study the structure of the genome in real time.DepositorAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Zinc Finger Consortium Expression Vector Kit v1.0
Plasmid Kit#1000000010PurposeContains plasmids and strains for the bacterial two-hybrid (B2H) system used to screen zinc finger arrays for activity. Also called the Modular Assembly Accessory Reagents Kit.DepositorAvailable SinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
ScEnSor Kit
Plasmid Kit#1000000215PurposeThis three-step (Build - Transform - Assess) Saccharomyces cerevisiae kit is comprised of two modules (Engineering and Biosensor) that can be used independently or in combination.DepositorAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Wnt Open Source
Plasmid Kit#1000000022PurposeOpen Source Wnt: 95 plasmid library of all 19 Wnt clones. Collection contains full-length open reading frames (ORFs) for every human Wnt ligand.DepositorAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only