4,932 results

1. pAcGHLT-B-DDB1

   Plasmid

   #48638

   Purpose

   Expresses Full Lenght DDB1 in Insect Cells (ampR)

   Depositor

   Ning Zheng

   Article

   Li et al Cell. 2006 Jan 13;124(1):105-17.

   Insert

   DDB1 (DDB1 Human)
   

   Expression

   Insect

   Available Since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pP{ELAV-GeneSwitch}

   Plasmid

   #83957

   Purpose

   Expresses conditional RU486-dependent GAL4 protein under control of the elav promotor

   Depositor

   Haig Keshishian

   Article

   Osterwalder et al Proc Natl Acad Sci U S A. 2001 Oct 23;98

   Insert

   fusion protein consisting of the GAL4-DBD, human progesterone receptor-ligand-binding domain, and P65-AD (GAL4, RELA Human, Budding Yeast)
   

   Expression

   Insect

   Promoter

   elav

   Available Since

   Dec. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-BbsI-chiRNA

   Plasmid

   #45946

   Purpose

   Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

   Depositor

   Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

   Article

   Gratz et al Genetics. 2013 May 24.

   Insert

   U6-BbsI-chiRNA
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   Dm-snRNA:U6:96Ab

   Available Since

   June 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTriEx4-H6-FGAm (FlincG3)

   Plasmid

   #49202

   Purpose

   Fluorescent reporter for cGMP

   Depositor

   John Garthwaite

   Article

   Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

   Insert

   FlincG3
   

   Tags

   His tag + other tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Met to Lys in cpEGFP region similar to CGaMP3

   Promoter

   CMV

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAC-GAL4

   Plasmid

   #24344

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   GAL4
   

   Expression

   Insect

   Available Since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGBdest-GST-PI3Kc1

   Plasmid

   #187992

   Purpose

   Plasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326

   Depositor

   Sascha Martens

   Inserts

   ATG14 (ATG14 Human)
   VPS34 (PIK3C3 Human)
   VPS15 (PIK3R4 Human)
   BECN1 (BECN1 Human)
   

   Tags

   GST

   Expression

   Bacterial and Insect

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFBOH-MHL_DCAF11:1-546

   Plasmid

   #210907

   Purpose

   Insect expression of full-length human DCAF11. N-terminal 6X His tag with TEV cleavage.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   DCAF11:M1-Q546 (DCAF11 Human)
   

   Tags

   MHHHHHHSSGRENLYFQG

   Expression

   Insect

   Mutation

   R207H natural variant (VAR_020121)

   Promoter

   Polyhedrin

   Available Since

   March 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. mEos3-Rac1

   Plasmid

   #92039

   Purpose

   Expresses mEos3-Rac1 fusion protein

   Depositor

   Ji Yu

   Article

   Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

   Insert

   RAC1 (RAC1 Human)
   

   Tags

   mEos3

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEos3-dnRac1

   Plasmid

   #92040

   Purpose

   Expresses mEos3 fusion with dnRac1 (T17N).

   Depositor

   Ji Yu

   Article

   Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

   Insert

   RAC1 (RAC1 Human)
   

   Tags

   mEos3

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T17N (dominant negative)

   Promoter

   CMV

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. mEos3-caRac1

    Plasmid

    #92041

    Purpose

    Expresses mEos3 fusion with caRac1 (Q61L)

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q61L (constitutively active)

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLIB-Cyclin K

    Plasmid

    #231718

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    CCNK (CCNK Human)
    

    Expression

    Insect

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4-PSMA5-PSMA6-PSMA7

    Plasmid

    #214138

    Purpose

    Insect cell expression of human 20S CP alpha-subunits

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMA1 (PSMA1 Human)
    PSMA2 (PSMA2 Human)
    PSMA3 (PSMA3 Human)
    PSMA4 (PSMA4 Human)
    PSMA5 (PSMA5 Human)
    PSMA6 (PSMA6 Human)
    PSMA7 (PSMA7 Human)
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWALIUM10-GW-TurboID-V5

    Plasmid

    #196283

    Purpose

    Gateway destination vector to express C-terminal TurboID fusion under UAS promoter, for making transgenic flies

    Depositor

    Norbert Perrimon

    Type

    Empty backbone

    Use

    Gateway destination vector

    Expression

    Insect

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFD8

    Plasmid

    #140619

    Purpose

    Expression of one of several LbCas12a crRNAs in Drosophila

    Depositor

    Michael Boutros

    Article

    Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    Drosophila U6:3

    Available Since

    Oct. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMT-puro

    Plasmid

    #17923

    Purpose

    Insect expression vector

    Depositor

    David Sabatini

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Drosophila metallothionein gene promoter drives e…

    Expression

    Insect

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGRACE

    Plasmid

    #230910

    Purpose

    Tracing of CRISPR activity in whole animals.

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    GFP
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFBOH-MHL_CDC20:161-477

    Plasmid

    #210943

    Purpose

    Insect expression of human CDC20 fragment S161 to P477. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CDC20:S161-P477 (CDC20 Human)
    

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available Since

    March 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pFBD-BirA_CDC20:161-P477

    Plasmid

    #210888

    Purpose

    Insect expression of human CDC20 fragment S161 to P477. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CDC20:S161-P477 (CDC20 Human)
    

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available Since

    March 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pHY002_Strep_Avi_hsDCAF15

    Plasmid

    #174479

    Purpose

    Expresses DCAF15 in insect cells

    Depositor

    Eric Fischer

    Article

    Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

    Insert

    DCAF 15 (DCAF15 Human)
    

    Tags

    StrepII-Avi-TEV

    Expression

    Insect

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWALIUM10-V5-TurboID-GW

    Plasmid

    #196285

    Purpose

    Gateway destination vector to express N-terminal TurboID fusion under UAS promoter, for making transgenic flies

    Depositor

    Norbert Perrimon

    Type

    Empty backbone

    Use

    Gateway destination vector

    Expression

    Insect

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHACK-GAL4>QF2

    Plasmid

    #80275

    Purpose

    GAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.

    Depositor

    Christopher Potter

    Article

    Lin et al Genetics. 2016 Jun 22. pii: genetics.116

    Insert

    QF2
    

    Expression

    Insect

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFastBac1-pGC-A

    Plasmid

    #186626

    Purpose

    pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)

    Depositor

    Debra Hansen

    Article

    Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1

    Insert

    NPR1 (NPR1 Human)
    

    Tags

    hemagglutinin (HA) signal peptide + His10-tag + T…

    Expression

    Insect

    Mutation

    deleted amino acids 1-32

    Promoter

    polyhedring

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFastBac-ATG101-ATG13 HORMA

    Plasmid

    #99332

    Purpose

    Mammalian expression of one subcomplex from the Human ULK1 complex

    Depositor

    James Hurley

    Article

    Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

    Inserts

    ATG101 (ATG101 Human)
    ATG13 (ATG13 Human)
    

    Tags

    GST

    Expression

    Insect

    Promoter

    p10 and pH

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBPnlsLexA::GADflUw

    Plasmid

    #26232

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    nlsLexA::GADfl
    

    Expression

    Insect

    Available Since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pWALIUM-intron 3x UAS attB

    Plasmid

    #206360

    Purpose

    includes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequences

    Depositor

    Erdem Bangi

    Article

    Teague et al PLoS Genet. 2023 Jun 2;19(6):e1010792. d

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFBOH-MHL_EIF3B:127-814

    Plasmid

    #210909

    Purpose

    Insect expression of human EIF3B fragment D127 to E814. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    EIF3B:D127-E814 (EIF3B Human)
    

    Tags

    MHHHHHHSSGRENLYFQGMQ

    Expression

    Insect

    Mutation

    Y760C sequence conflict

    Promoter

    Polyhedrin

    Available Since

    March 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. Orco-T2A-QF2-9xQUAS-GCaMP6f-3XP3-dsRed

    Plasmid

    #157974

    Purpose

    Template plasmid for inserting GCaMP reporter into the Orco gene of Aedes aegypti with CRISPR/Cas9

    Depositor

    Carolyn McBride

    Article

    Zhao et al Nature. 2022 May;605(7911):706-712. doi:

    Insert

    Orco
    

    Expression

    Insect

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Opie2-PuroR

    Plasmid

    #131613

    Purpose

    Expresses PuroR and dsRed.

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

    Inserts

    PuroR
    dsRed
    

    Expression

    Insect

    Promoter

    Opie2 and hr5Ie1

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBPp65ADZpUw

    Plasmid

    #26234

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    p65AD-Zip (RELA Human)
    

    Expression

    Insect

    Available Since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJFRC81-10XUAS-IVS-Syn21-GFP-p10

    Plasmid

    #36432

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Proc Natl Acad Sci U S A. 2012 Apr 9.

    Insert

    GFP
    

    Expression

    Insect

    Available Since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAcBac1.tR4-MbPyl

    Plasmid

    #50832

    Purpose

    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

    Depositor

    Peter Schultz

    Article

    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

    Inserts

    two-copy Pyl tRNA cassette
    MbPyl
    two copy Pyl tRNA cassette
    

    Use

    Baculoviral

    Expression

    Insect

    Promoter

    CMV and U6 and H1

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pACEBac1-PARP2-Flag-His-WT

    Plasmid

    #111574

    Purpose

    Expresses full length PARP2 in Sf9 (insect) cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP2 (PARP2 Human)
    

    Tags

    C-terminal 6xHis and C-terminal Flag

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSSRB069_pAC8-hsDDB1dB

    Plasmid

    #218790

    Purpose

    Insect cell expression vector for 6xHis-TEV-hsDDB1∆B

    Depositor

    Eric Fischer

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    DNA damage-binding protein 1 (DDB1 Human)
    

    Tags

    6xHis-TEV

    Expression

    Insect

    Mutation

    Deleted amino acids 396-705 and replaced with GNG…

    Promoter

    polyhedrin

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBS130

    Plasmid

    #26290

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    phiC31 integrase (int Streptomyces phage phiC31)
    

    Use

    Drosophila transgenesis

    Expression

    Bacterial and Insect

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCoPURO

    Plasmid

    #17533

    Depositor

    Francis Castellino

    Article

    Iwaki et al Biotechniques. 2003 Sep . 35(3):482-4, 4

    Insert

    Puromycine Acetyl Transferase
    

    Expression

    Insect

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFBD-Akt1(D323A/D325A)

    Plasmid

    #86592

    Purpose

    Expresses human full-length Akt1(D323A/D325A) mutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Mutation

    D323A/D325A transforming mutations

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

    Plasmid

    #111903

    Purpose

    Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.

    Depositor

    Andrew Carter

    Inserts

    His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
    DYNC1I2 (DYNC1I2 Human)
    DYNC1LI2 (DYNC1LI2 Human)
    DYNLT1 (DYNLT1 Human)
    DYNLRB1 (DYNLRB1 Human)
    DYNLL1 (DYNLL1 Human)
    

    Tags

    8xHis, SNAPf-tag, TEV site, and ZZ-tag

    Expression

    Insect

    Mutation

    Optimised for expression in Sf9 cells.

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAC-14-LEXA-QF

    Plasmid

    #46104

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    LexA-QF
    

    Expression

    Insect

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pIZ-Flag6His-BmAgo3

    Plasmid

    #50559

    Purpose

    Expresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cells

    Depositor

    Yukihide Tomari

    Article

    Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

    Insert

    BmAgo3
    

    Tags

    6xHIS and Flag

    Expression

    Insect

    Promoter

    OpIE2

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. ClCry4 PHR

    Plasmid

    #160552

    Purpose

    Expression of protein for various experiments including finding interaction partners

    Depositor

    Joseph Takahashi

    Article

    Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

    Insert

    Clcry4 HR
    

    Tags

    Hexa his

    Expression

    Insect

    Available Since

    Nov. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge