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Yeast Expression

8,025 results

Showing: 421 - 450 of 8025 results

  1. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid
    #91249
    Purpose
    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    91249-plasmid-map-sequence-id-181276

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  2. pCEV-G2-Km ymNeongreen

    Plasmid
    #193959
    Purpose
    TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymNeongreen in budding yeast
    Depositor
    Bas Teusink
    Insert
    ymNeonGreen
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    193959-plasmid-map-sequence-id-383894

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  11. p406GAL1

    Plasmid
    #17437
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    GAL1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17437-plasmid-map-sequence-id-8611

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  14. PGAL

    Plasmid
    #60337
    Purpose
    Contains full length native galactose promoter
    Depositor
    Hal Alper
    Article
    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884
    Inserts
    promoter
    yECitrine
    Use
    Synthetic Biology; Yeast synthetic hybrid promote…
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 6, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    60337-plasmid-map-sequence-id-474139

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  17. pWZ699

    Plasmid
    #208117
    Purpose
    Payload yeast assembly vector with TK-mActb on backbone
    Depositor
    Jef Boeke
    Article
    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-
    Type
    Empty backbone
    Use
    Synthetic Biology; Copy number inducible
    Tags
    Expression
    Bacterial, Mammalian, and Y…
    Mutation
    Promoter
    Human PGK1 for thymidine kinase
    Available since
    Nov. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    208117-plasmid-map-sequence-id-409744

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  21. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid
    #73796
    Purpose
    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA
    Depositor
    Ronald Davis
    Article
    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1
    Inserts
    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    Use
    CRISPR
    Tags
    Mxi1 and NLS
    Expression
    Yeast
    Mutation
    D10A, H840A
    Promoter
    pGPM1, pRPR1(TetO), and pTef1
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73796-plasmid-map-sequence-id-237125

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  26. pNSU299_GAL2

    Plasmid
    #166088
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GAL2 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    GAL2 (GAL2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166088-plasmid-map-sequence-id-329112

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  27. pCL28-decoder

    Plasmid
    #159450
    Purpose
    Insert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    PlexAtet-LexA-ER-B112-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PlexAtet
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159450-plasmid-map-sequence-id-313198

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  28. pCL66-decoder

    Plasmid
    #159453
    Purpose
    Insert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    Pfus1mut-tetR-nls-malE-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Pfus1mut
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159453-plasmid-map-sequence-id-313208

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  29. pRS415-GPD

    Plasmid
    #183940
    Purpose
    Control vector that has a GPD promoter with no insert
    Depositor
    Michael Downey
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183940-plasmid-map-sequence-id-363367

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Showing: 421 - 450 of 8025 results