166,552 results

1. BPK1520

   Plasmid

   #65777

   Purpose

   Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SpCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

   Plasmid

   #67974

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2ABFP cassette, WPRE
   

   Use

   Lentiviral

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. SPLICS PM-ER Long P2A

   Plasmid

   #164111

   Purpose

   Detect the long Plasma membrane-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP PM-ER Long P2A
   

   Expression

   Mammalian

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Myd88-CFP

   Plasmid

   #13026

   Depositor

   Doug Golenbock

   Insert

   myeloid differentiation primary response gene (88) (MYD88 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPLICS PM-ER Short P2A

   Plasmid

   #164112

   Purpose

   Detect the short Plasma membrane-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP PM-ER Short P2A
   

   Expression

   Mammalian

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. mTagBFP2-pBAD

   Plasmid

   #54572

   Purpose

   Localization: Protein Expression Vector , Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   6xHis, T7, and mTagBFP2

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. lentiCRISPRv2 hygro

   Plasmid

   #98291

   Purpose

   Variant of lentiCRISPRv2 that confers hygromycin resistance

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Promoter

   EF-1a

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNeonGreen-C1-Nir1-LNS2

   Plasmid

   #220083

   Purpose

   High affinity PA biosensor with green fluorophore (also known as NeonGreen-PILS-Nir1)

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   PITPNM3 (PITPNM3 Human)
   

   Tags

   NeonGreen-GGSGGM

   Expression

   Mammalian

   Mutation

   amino acids 613-897

   Promoter

   CMV

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-DIO-BACE-HA

   Plasmid

   #232353

   Purpose

   Beta-Secretase to be expressed (1:1) with either ATLAS protein.

   Depositor

   Don Arnold

   Article

   Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

   Has Service

   AAV8

   Insert

   BACE (BACE1 Human)
   

   Use

   AAV

   Tags

   HA

   Promoter

   hSyn

   Available Since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. emiRFP670-cGAS

    Plasmid

    #231896

    Purpose

    Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    cGAS (CGAS Human)
    

    Tags

    emiRFP670

    Expression

    Mammalian

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-gp78-FLAG

    Plasmid

    #62370

    Purpose

    Express C-terminally FLAG tagged gp78

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    gp78 (AMFR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    *

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. p21-Reporter

    Plasmid

    #52432

    Purpose

    DNA damage inducible Red Fluorescent Protein for mammalian expression

    Depositor

    Joel Voldman

    Article

    Fendyur et al Anal Chem. 2014 Aug 5;86(15):7598-605. d

    Insert

    p21-p53 Tandem Response Element Promoter
    

    Tags

    TurboRFP

    Expression

    Mammalian

    Promoter

    13X repeated p21-p53 response element

    Available Since

    Oct. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRN11

    Plasmid

    #84455

    Purpose

    shuttle plasmid for sarA P1-mCherry expression

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    mCherry
    

    Use

    Shuttle vector e.coli-s.aureus, expression of sgf…

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. synIRES_RC25

    Plasmid

    #189970

    Purpose

    Part 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    synIRES_RC25
    

    Use

    Cloning

    Promoter

    N/A

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTIPI_mIGg2a_Anti-HA [12CA5]

    Plasmid

    #210656

    Purpose

    Mammalian expression of Anti-HA [12CA5] mouse IgG2a heavy chain; to be used with pTIPI_mK_Anti-HA [12CA5] light chain (Plasmid 210657) to make the antibody.

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Anti-HA [12CA5] mouse IgG2a heavy chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Mouse IgG2a heavy chain

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pTIPI_mK_Anti-HA [12CA5]

    Plasmid

    #210657

    Purpose

    Mammalian expression of Anti-HA [12CA5] mouse kappa light chain; to be used with pTIPI_mIGg2a_Anti-HA [12CA5] heavy chain (Plasmid 210656) to make the antibody.

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Anti-HA [12CA5] kappa light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Mouse kappa light chain

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pAAV-CAG-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234451

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. murine TCR OTI-2A.pMIG II

    Plasmid

    #52111

    Purpose

    retroviral expression of both TCRalpha and TCRbeta using a single vector

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Inserts

    T cell receptor alpha chain (Tcra Mouse)
    T cell receptor beta chain (Tcrb Mouse)
    

    Use

    Retroviral

    Tags

    T2A (from Thosea asigna virus)

    Expression

    Mammalian

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. HP17_KO7

    Plasmid

    #120346

    Purpose

    Helper plasmid for production of phagemid ssDNA, based on M13KO7

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

    Insert

    Coding part of the M13KO7 helper phage genome
    

    Expression

    Bacterial

    Promoter

    native phage promoters

    Available Since

    Aug. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid

    #107942

    Purpose

    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    

    Tags

    3x HA and NLS (nucleoplasmin)

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R, and N282A

    Promoter

    CAG

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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