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Plasmid#206281PurposeENTR Vector 1 for MultiSite Gateway assembly. Encodes mAG-hGeminin under the control of CMVDepositorInsertmAG-hGeminin (GMNN Human)
UseMultimate/gateway entr 1TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 1 CMV mAG beta-catenin
Plasmid#206258PurposeENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mAG Ctnnb1 under the control of CMV promoter.DepositorInsertmAG Ctnnb1 (Ctnnb1 Mouse)
UseMultimate/gateway entr 1Tagsmonomeric Azami GreenExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKM2 WT
Plasmid#107158PurposeMBP-rPKM2 WT for production and purification of rat PKM2 WTDepositorInsertMBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationPromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Mouse Ism1 with C-terminal Myc-6X-his tag
Plasmid#173046PurposeExpresses Ism1 in mammalian cellsDepositorInsertIsm1 (Ism1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
PEA1-Nuclease-Puro HEK3 CTT ins
Plasmid#171995PurposeDelivers all prime editing nuclease components targeting the HEK3 gene for a CTT inserrtion, in a single, puromycin selectable plasmidDepositorInsertHEK3 CTT insertion pegRNA and CbH-Cas9-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA sham
UseTagsExpressionMammalianMutationPromoterCMV for Cas9, U6 for gRNAsAvailable sinceSept. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKL WT
Plasmid#107156PurposeMBP-rPKL WT for production and purification of rat PKL WTDepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationPromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-hPKL S113D
Plasmid#107161PurposeFor mammalian expression of Flag-hPKL S113DDepositorInsertFlag-hPKL S113D (PKLR Human)
UseRetroviralTagsN-term FlagExpressionMammalianMutationSerine 113 mutated to aspartatePromoterCMVAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-hPKL S113A
Plasmid#107160PurposeFor mammalian expression of Flag-hPKL S113ADepositorInsertFlag-hPKL S113A (PKLR Human)
UseRetroviralTagsN-term FlagExpressionMammalianMutationSerine 113 mutated to alaninePromoterCMVAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKL S12TAG
Plasmid#107155PurposeMBP-rPKL construct for production and purification of rat PKL pS12DepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationSerine 12 to TAG for phosphoserine insertionPromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKL S113TAG
Plasmid#107157PurposeMBP-rPKL S113TAG for production and purification of rat PKL pS113DepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationSerine 113 changed to TAG for phosphoserine inser…PromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
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7M3X
Plasmid#176411PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertRBBP7 (RBBP7 Human)
UseBaculovirus expresssionTags6xHis-TEVExpressionMutationPromoterPolyhedrinAvailable sinceOct. 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
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5KKP (PUS7)
Plasmid#101878PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPUS7 (PUS7 Human)
UseTags6xHis, TEVExpressionBacterialMutationPromoterT7Available sinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
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5TF2 (PREB)
Plasmid#101889PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertPREB (PREB Human)
UseBaculovirus expressionTags6xHis, TEVExpressionMutationPromoterpolyhedrinAvailable sinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
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MAGEA3
Plasmid#73246PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMAGEA3 (MAGEA3 Human)
UseTagsHis6-Thioredoxin-TEVExpressionBacterialMutationpfam01454:MAGE 116-286PromoterT7Available sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
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STARD13A
Plasmid#42384PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSTARD13A (STARD13 STARD13A, Human)
UseTagsHis6-TEVExpressionBacterialMutationSee CommentsPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pAc5.1B-V5-SBP-DmNot2_K
Plasmid#146721PurposeInsect Expression of DmNot2DepositorInsertDmNot2 (Rga Fly)
UseTagsExpressionInsectMutationone silent mutation C267T compared to the ORF ext…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
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5UBA (RPUSD4)
Plasmid#101256PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRPUSD4 (RPUSD4 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
5VBB (RPUSD1)
Plasmid#101259PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRPUSD1 (RPUSD1 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
5C7J
Plasmid#69965PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertNEDD4:SDC094-F02:C29544 (NEDD4 Human)
UseTagsHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceOct. 7, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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GYG2
Plasmid#73254PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGYG2 (GYG2 Human)
UseTagsTEV-His6-FlagExpressionBacterialMutationpfam01501:Glyco_transf_8 8-224PromoterT7Available sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
4Z0O
Plasmid#69954PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertZCWPW2:JMC01B-H12:C203050 (ZCWPW2 Human)
UseTagsHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceOct. 7, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
4YVD
Plasmid#69960PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertPLRG1:JMC102-C09:C233452 (PLRG1 Human)
UseBaculovirus expressionTagsHis and TEV cleavage siteExpressionMutationPromoterpolyhedrinAvailable sinceOct. 7, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -