We narrowed to 2,357 results for: Efs

1. pRDA_886

   Plasmid

   #201162

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti_gRNA-Puro

   Plasmid

   #84752

   Purpose

   Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. AsCas12a-6xNLS-E174R/S542R (pRG232)

   Plasmid

   #149723

   Purpose

   Lentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance gene

   Depositor

   Junwei Shi

   Article

   Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIS1-Eef25UTR-TOPmut-renilla

   Plasmid

   #38236

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   Eef2_TOPm_5UTR/Renilla (Eef2 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   The first nucleotides of the 5' UTR were cha…

   Promoter

   Endogenous Eef2

   Available since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pGP-CMV-NES-fRCaMP2

   Plasmid

   #125250

   Purpose

   Expresses red fluorescent calcium sensor in mammalian cells

   Depositor

   Katalin Torok

   Article

   Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

   Insert

   fRCaMP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D396A

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE2-EF1a-HA-ATAD3A-IRES-Hygro

   Plasmid

   #211469

   Purpose

   Lentivirus backbone expressing ATAD3A with an HA-tag at the N-terminal

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   ATAD3A (ATAD3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-FLIM-AKAR

   Plasmid

   #63058

   Purpose

   Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backbone

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

   Insert

   FLIM-AKAR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAXEF-Rap1-WT

   Plasmid

   #32697

   Depositor

   Vicki Boussiotis

   Article

   Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

   Insert

   RAP1 (RAP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF promoter

   Available since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

9. LRG2.1_Puro

   Plasmid

   #125594

   Purpose

   Lentiviral expression of sgRNA with GFP and puromycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for sgRNA expression and EFS promoter…

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDA_888

    Plasmid

    #201165

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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