We narrowed to 7,456 results for: pac

1. pAcGFP1-N1-MAHS

   Plasmid

   #90034

   Purpose

   To express GFP-tagged MAHS in mammalian cells transiently

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

   Insert

   MAHS
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACT2-μ3A

   Plasmid

   #198174

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-3 μ3A
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   silent substitution in codon 225

   Promoter

   ADH1

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACT2-μ3B

   Plasmid

   #198175

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-3 μ3B
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pL-SFFV.Reporter.RFP657.PAC

   Plasmid

   #61395

   Purpose

   Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAC-crRNA-Cm

   Plasmid

   #158711

   Purpose

   Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   eforCP (for crRNA cloning)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetO

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAcGFP1-N1-Dsup-C

   Plasmid

   #90022

   Purpose

   To express GFP-tagged C-terminal region of Dsup in mammalian cells transiently

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   C-terminal region alone

   Promoter

   CMV

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC1817_pCR8-gSMN2-EX

   Plasmid

   #118651

   Purpose

   Transient transfection; Expressed SMN2-EX CasRx gRNA; Gateway Donor

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   gSMN2-EX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACYC-GFPM2

   Plasmid

   #102791

   Purpose

   Expresses GFP under WT T7 promoter

   Depositor

   Andrew Ellington

   Article

   Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

   Insert

   Green Fluorescent Protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAct-GW-HygroR

   Plasmid

   #149610

   Purpose

   Gateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   Gateway cassette (ccdb + Chlor.R.)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Act5c

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF

    Plasmid

    #82021

    Purpose

    cLac143; pALM143; IPTG inducible system

    Depositor

    Brian Pfleger

    Article

    Markley et al ACS Synth Biol. 2015 May 15;4(5):595-603

    Insert

    YFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Pcptoo_C38T,A39T

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACEBac1-GCP4

    Plasmid

    #178070

    Purpose

    Insect cell expression of GCP4

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    GCP4 (TUBGCP4 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAcGHLT-B-DDB1

    Plasmid

    #48638

    Purpose

    Expresses Full Lenght DDB1 in Insect Cells (ampR)

    Depositor

    Ning Zheng

    Article

    Li et al Cell. 2006 Jan 13;124(1):105-17.

    Insert

    DDB1 (DDB1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pACT2-CAS9c

    Plasmid

    #51055

    Purpose

    Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    Cas9c
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    yeast ADH1 promoter

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pActinin-miRFP703

    Plasmid

    #79992

    Purpose

    Alpha actinin labeling with near-infrared fluorescent protein miRFP703

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    Alpha-Actinin (ACTN1 Human)
    

    Use

    Tags

    miRFP703

    Expression

    Mammalian

    Mutation

    Promoter

    c

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPac Sp1

    Plasmid

    #12095

    Depositor

    Robert Tjian

    Article

    Courey et al Cell. 1988 Dec 2. 55(5):887-98.

    Insert

    Sp1 (SP1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pActPL-Gal4DBD

    Plasmid

    #15304

    Depositor

    Benjamin White

    Article

    Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

    Insert

    ZIP- Gal4DBD (GAL4 )
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc5.1B-lambdaN-HA

    Plasmid

    #21302

    Depositor

    Elisa Izaurralde

    Article

    Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

    Insert

    lambdaN-HA
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAC-crRNA-Km

    Plasmid

    #158712

    Purpose

    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    AcGFP (for crRNA cloning)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACUH-sfcherry2(1-10)

    Plasmid

    #172061

    Purpose

    UAS vector to express sfcherry2(1-10) in Drosophila

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    sfcherry2(1-10)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. IL2 pAcGP67a

    Plasmid

    #41806

    Depositor

    Chris Garcia

    Article

    Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

    Insert

    IL2 (IL2 Human)
    

    Use

    Tags

    6x His

    Expression

    Insect

    Mutation

    Promoter

    Polyhedron

    Available since

    Jan. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pET28a-ec_bPAC

    Plasmid

    #28135

    Purpose

    Expression of photoactivated cyclase bPAC of Beggiatoa sp. optimized for E. coli

    Depositor

    Peter Hegemann

    Article

    Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

    Insert

    E.coli adapted photoactivated adenylyl cyclase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pACM4-ACA

    Plasmid

    #49809

    Purpose

    Encodes fabD, accA, accB, accC, accD in pseudo-operon configuration.

    Depositor

    Mattheos Koffas

    Article

    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

    Inserts

    ACP transacylase
    Acetyl-CoA carboxylase
    acetyl-CoA carboxylase
    Acetyl-CoA carboxylase
    Acetyl-CoA carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent site-directed mutagenesis to remove SalI s…

    Promoter

    T7

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAct::Raf

    Plasmid

    #11341

    Depositor

    Michael Wigler

    Article

    Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

    Insert

    byr-2 (byr2 Fission Yeast)
    

    Use

    Tags

    Gal4 activation domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pACEBac1-GCP6

    Plasmid

    #178072

    Purpose

    Insect cell expression of GCP6

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    GCP6 (TUBGCP6 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMC051-HisMS2_PLP_pac_LC

    Plasmid

    #128234

    Purpose

    Lower Copy expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

    Depositor

    Paul Freemont

    Article

    de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

    Inserts

    Maturation Protein
    Coat Protein Dimer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 15, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pActin-Electra1

    Plasmid

    #184941

    Purpose

    beta-actin labelling with blue fluorescent protein Electra1

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra1-beta-Actin
    

    Use

    Tags

    Electra1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pActPL-Gal4AD

    Plasmid

    #15303

    Depositor

    Benjamin White

    Article

    Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

    Insert

    Gal4AD-ZIP+ (GAL4 )
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pACgp67B-Her2mP

    Plasmid

    #10793

    Depositor

    Judy Lieberman

    Article

    Song et al Nat Biotechnol. 2005 Jun . 23(6):709-17.

    Insert

    ErbB2 ScFv (ERBB2 Human)
    

    Use

    Tags

    6His and Truncated Protamine

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX165-HypaCas9

    Plasmid

    #140567

    Purpose

    Expresses HypaCas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    N692A M694A Q695A H698A

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pACT-Lex

    Plasmid

    #183149

    Purpose

    Yeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)

    Depositor

    Assaf Mosquna

    Article

    Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

    Insert

    GAL4 AD
    

    Use

    Yeat two-hybrid

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge