We narrowed to 31,888 results for: Eng

1. pCon-IFl_Con-ICr

   Plasmid

   #192211

   Purpose

   Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Consensus I-F Leader and Consensus I-C Locus DNA
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCon-IFl_Con-IIAr

   Plasmid

   #192212

   Purpose

   Consensus I-F CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Consensus I-F Leader and Consensus II-A Locus DNA
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCon-IEl_Con-IEr

   Plasmid

   #192213

   Purpose

   Consensus I-E CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Consensus I-E Leader and Consensus I-E Locus DNA
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCon-ICl_Con-ICr

   Plasmid

   #192214

   Purpose

   Consensus I-C CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Consensus I-C Leader and Consensus I-C Locus DNA
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCon-IIAl_Con-IIAr

   Plasmid

   #192215

   Purpose

   Consensus II-A CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Consensus II-A Leader and Consensus II-A Locus DNA
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX1-IFl_Con-IFr

   Plasmid

   #192216

   Purpose

   Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   I-F leader and I-F repeat construct not studied further in this paper (X1)
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX2-IFl_Con-IFr

   Plasmid

   #192217

   Purpose

   Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   I-F leader and I-F repeat construct not studied further in this paper (X2)
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCHC030 (∆CBBp ∆MBH ∆SH)

   Plasmid

   #226205

   Purpose

   Electroporation knockout plasmid (pK18sB) to delete the CBBp, membrane-bound hydrogenase, soluble hydrogenase, and intervening sequences (∆CBBp ∆MBH ∆SH).

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for CBBp, MBH, and SH operons, and intervening sequencessequences.
   

   Use

   Synthetic Biology

   Mutation

   ∆cbbR ∆cbbLSXYEFPTZGKA ∆hoxKGZMLOQRTV ∆hypA1B1F1C…

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCHC036 (∆pHG1)

   Plasmid

   #226206

   Purpose

   Conjugation knockout plasmid (pK18msB) to delete the C. necator megaplasmid replication region containing helD, repA, repB, and parAB in order to promote loss of the entire pHG1 megaplasmid.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for the pHG1 megaplasmid replication region
   

   Use

   Synthetic Biology

   Mutation

   ∆helD ∆repA ∆repB ∆parAB

   Available Since

   Nov. 14, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pACB120

    Plasmid

    #222213

    Purpose

    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with five mutations (K10N, S11R, W15C, G76V, G258D) and a C-terminal His tag

    Depositor

    Gregg Beckham

    Article

    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

    Insert

    fghA(K10N, S11R, W15C, G76V, G258D)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Contains five amino acid mutations: K10N, S11R, W…

    Promoter

    T7

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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