We narrowed to 31,888 results for: Eng

1. pRAW-460

   Plasmid

   #200216

   Purpose

   Strep-TEV-PaCas1 + PaCas2/3 (Cas2 R55E, N56D)

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   PaCas1, PaCas2-3
   

   Tags

   Strep II-TEV on Cas1

   Expression

   Bacterial

   Mutation

   Cas2 R55E-N56D mutations

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRAW-461

   Plasmid

   #200217

   Purpose

   Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E)

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   PaCas1, PaCas2-3
   

   Tags

   Strep II-TEV on Cas1

   Expression

   Bacterial

   Mutation

   Cas2 K11D-R12E mutations

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRAW-462

   Plasmid

   #200218

   Purpose

   Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E, R55E, N56D)

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   PaCas1, PaCas2-3
   

   Tags

   Strep II-TEV on Cas1

   Expression

   Bacterial

   Mutation

   Cas2 K11D-R12E, R55E-N56D mutations

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. (B) G418 marker (R) (SBE 137)

   Plasmid

   #195011

   Purpose

   geneticin (G418) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOS

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   pXYL+G418+tNOS
   

   Expression

   Bacterial

   Promoter

   pXYL

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Coexpressed Free NES

   Plasmid

   #182482

   Purpose

   Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Inserts

   FPPS
   NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL10 and GAL7

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Coexpressed Free PcPTS

   Plasmid

   #182483

   Purpose

   Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and patchoulol synthase from Pogostemon cablin (PcPTS; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Inserts

   FPPS
   PTS
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL10 and GAL7

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Linked Free NES (AG4TGGA)2

   Plasmid

   #182488

   Purpose

   Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence (AG4TGGA)2 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Insert

   FPPS-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL10

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Linked Free (PT)4P

   Plasmid

   #182490

   Purpose

   Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence PTPTPTPTP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Insert

   FPPS-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL10

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTS2326

   Plasmid

   #169628

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Expression

   Mammalian

   Promoter

   PhCMV / PPGK

   Available Since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTS2321

    Plasmid

    #169626

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Expression

    Mammalian

    Promoter

    PhCMV / PPGK

    Available Since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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