We narrowed to 5,270 results for: pcdna3.1
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hCathepsin S
Plasmid#11251PurposeMammalian expression of human Cathepsin SDepositorInsertcathepsin S (CTSS Human)
UseTagsC9 (GTETSQVAPA)ExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-HA-DNM2-R465W
Plasmid#155262PurposeMammalian expression of rat DNM2 fused to HADepositorInsertdynamin 2 (Dnm2 Rat)
UseTagsHAExpressionMammalianMutationArginine 465 to TryptophanPromoterAvailable sinceMay 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-LAR-GECO1.2
Plasmid#61245PurposeExpresses LAR-GECO1.2 in the mitochondria in mammalian cellsDepositorInsertLAR-GECO1.2
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationSubstitutions relative to R-GECO1.2: N45I/A47R/E1…PromoterCMVAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
EphA2-YFP
Plasmid#108852PurposeEncodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linkerDepositorInsertEPHA2 (EPHA2 Human)
UseTagsLabeled with eYFP on the C-Terminus via a 15 amin…ExpressionMammalianMutationPromoterAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD4
Plasmid#195842PurposeExpresses human FZD4 with an N-terminal HiBiTDepositorInsertFZD4 (FZD4 Human)
UseTagsHiBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
SacI ML GFP Strand 11 Long
Plasmid#164120PurposeDetect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10DepositorInsertSacI ML GFP Strand 11 Long
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
InsRA-eGFP
Plasmid#79795PurposeInsulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.DepositorInsertInsRA (INSR Human)
UseTagseGFP extracellularExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD2
Plasmid#195840PurposeExpresses human FZD2 with an N-terminal HiBiTDepositorInsertFZD2 (FZD2 Human)
UseTagsHiBiTExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_MET_D14_3Flag
Plasmid#182495PurposeContains cDNA of the MET gene without the exon 14DepositorInsertMET (MET Human)
UseTagsC-terminal 3xFlag tagExpressionMammalianMutationExon 14 deletedPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 TAK1/F
Plasmid#44161DepositorInsertTak1 (Map3k7 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
RANLS
Plasmid#50843PurposeExpresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.DepositorInsertddRFP A
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
GANLS
Plasmid#50852PurposeExpresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.DepositorInsertddGFP A
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
WN10150
Plasmid#80443PurposeExpresses dead (908A) AsCpf1DepositorInsertnuclease dead (908A) AsCpf1
UseTagsNLS-3xHAExpressionMammalianMutationnuclease dead (D908A)PromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2(WT)-8his
Plasmid#149268PurposeMammalian expression plasmid for his-tagged sACE2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 RNAi resistant
Plasmid#118347PurposeThis plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationRNAi resistance mutations (does not change amino …PromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
ER-mGreenLantern
Plasmid#164463PurposeLocalization and retention of mGreenLantern fluorescent protein in the ERDepositorInsertmGreenLantern
UseTagsCalreticulin and KDELExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HLA-cMyc-EcopT1R1
Plasmid#113962Purposemammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R1 (TAS1R1 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationtruncate N-terminal 24 residuesPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY30
Plasmid#84745PurposeExpresses huAsCpf1-P2A-puro and crRNA guideDepositorInsertshuAsCpf1
puromycin resistance gene
UseTags3xHA, NLS, and T2AExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1-FLAG
Plasmid#166803PurposeMammalian cell expression vector for FLAG-tagged NLRP1.DepositorInsertNLRP1 (NLRP1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SacI ML GFP Strand 11 Short
Plasmid#164121PurposeDetect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10DepositorInsertSacI ML GFP Strand 11 Short
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR146
Plasmid#106047PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR146; Opto-GPR146; E1)DepositorInsertOpto-GPR146 (GPR146 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNAZeo(-) LAMP2C
Plasmid#89342PurposeExpresses human LAMP-2C in mammalian cellsDepositorInsertLysosomal-associated membrane protein 2C (LAMP2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 proHB-EGF CS
Plasmid#11601DepositorInsertHB-EGF (HBEGF Human)
UseTagsHis and mycExpressionMammalianMutationConstitutively secreted HB-EGF: C-terminal amino …PromoterAvailable sinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED18
Plasmid#15419DepositorInsertMED18 (MED18 Human)
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 mitoAURKA
Plasmid#157750PurposeExpression of AURKA targeted to mitochondriaDepositorInsertAURKA (AURKA Human)
UseTagsExpressionMammalianMutationAurora A without the first 30AA + MTS of CoxVIIIPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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Myc-RILPL1
Plasmid#201470PurposeFor overexpression in cultured cells to visualize RILPL1 protein interactions.DepositorInsertRILPL1 (RILPL1 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124434PurposeExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in DrosophilaDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-his-BACH1 K52R
Plasmid#17643DepositorInsertBACH1 (BRIP1 Human)
UseTagsHis and MycExpressionMammalianMutationThe lysine residue at position 52 was changed to …PromoterAvailable sinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
CCM2_wt_eGFP
Plasmid#208876PurposeExpress eGFP-CCM2 in mammalian cells.DepositorInsertCCM2 (CCM2 Human)
UseTagseGFPExpressionMammalianMutationWT sequencePromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 S303/7A
Plasmid#118365PurposeThis plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationS303A and S307APromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEEF1D-FLAG (pcDNA 3.1+) LG6-1
Plasmid#37365DepositorInsertEEF1D (EEF1D Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA-dCas9-HA
Plasmid#61355Purposeencodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal HA tag
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
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CMV-mito-CAR-GECO1
Plasmid#46022PurposeCarmine intensiometric genetically encoded Ca2+-indicators for optical imagingDepositorInsertCAR-GECO1
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationR-GECO1 E163V/I166V/V174T/M176I/F222I/A302PPromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Opto-M1R
Plasmid#106069PurposeExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-M1R; Opto-M1R; F11; 722)DepositorInsertOpto-M1R (CHRM1 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD6
Plasmid#195844PurposeExpresses human FZD6 with an N-terminal HiBiT; contains M345LDepositorInsertFZD6 (FZD6 Human)
UseTagsHiBiTExpressionMammalianMutationM345LPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
OTR untagged
Plasmid#208913Purposeencodes the human oxytocin receptor, without any tagsDepositorInsertOXTR (OXTR Human)
UseTagsExpressionMammalianMutationTM5: 5x56, A218, Threonin instead of AlaninPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only
