We narrowed to 4,850 results for: U6...

1. SL3-MS2-gRNA GG backbone (MOBE3-4)

   Plasmid

   #219949

   Purpose

   Golden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   gRNA-MS2-SL3 (S. pyogenes Cas9)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3'-com-gRNA GG backbone (MOBE1-4)

   Plasmid

   #219947

   Purpose

   Golden gate backbone for mammalian expression of Sp-gRNA with 3'-com

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   gRNA-com-3'end (S. pyogenes Cas9)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 3'MS2-gRNA GG backbone (MOBE1-2)

   Plasmid

   #219948

   Purpose

   Golden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   gRNA-MS2-3'end (S. pyogenes Cas9)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgRNA TR KO 2

   Plasmid

   #207610

   Purpose

   sgRNA 2 to knockout TR by replacement with a PuroR cassette

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   TCAGGCCGCAGGAAGAGGAA
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. gRNA-hIRF-1 #12/pSIR-hCD2

   Plasmid

   #135392

   Purpose

   Expresses a guide RNA (gRNA) to target human IRF-1 promoter

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   gRNA_hIRF1 promoter #12
   

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgRNA TR KO 1

   Plasmid

   #207609

   Purpose

   sgRNA 1 to knockout TR by replacement with a PuroR cassette

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   ACCCTAACTGAGAAGGGCGT
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO.1-TST-EGFP-GPIDAF

   Plasmid

   #213708

   Purpose

   For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an hPGK promoter.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   Tags

   Twin-strep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1-TST-EGFP-GPIBY55

   Plasmid

   #213707

   Purpose

   For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an hPGK promoter.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   Tags

   Twin-strep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. hLig4 guide2 in pX458

   Plasmid

   #211536

   Purpose

   sgRNA-2 against Lig4

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   sgRNA hLig4 (LIG4 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   u6

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. hLig4 guide1 in pX458

    Plasmid

    #211535

    Purpose

    sgRNA-1 against Lig4

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    sgRNA hLig4 (LIG4 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    u6

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAf-CRISPR-ctfR1

    Plasmid

    #207992

    Purpose

    CRISPR vector used with pAf-CRISPR-phoA (#207991) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

    Insert

    ctfR1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Aspergillus flavus U6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACBE-ASR (pXK-607)

    Plasmid

    #208822

    Purpose

    Universal Antibiotic Screening Reporter for adenine and cytosine base editors. Key Construct: CMV-PuroR*(ATAACG)-eGFP-hU6-gRNA.

    Depositor

    Kun Xu

    Article

    Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi

    Type

    Empty backbone

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pACBE-FSR (pXK-1648)

    Plasmid

    #208821

    Purpose

    Universal Fluorescent Surrogate Reporter for adenine and cytosine base editors. Key Construct: CMV-mCherry-CMV-eGFP* (ATAACG)-hU6-gRNA.

    Depositor

    Kun Xu

    Article

    Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi

    Type

    Empty backbone

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZCS41 (U6p::GCGAAGTGACGGTAGACCGT)

    Plasmid

    #193050

    Purpose

    Encodes guide RNA expression targeting PX740 landing pad

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    Guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. lenti-Cas9-sgHPRT1

    Plasmid

    #196713

    Purpose

    CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

    Depositor

    Bernhard Schmierer

    Article

    Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

    Insert

    Cas9-T2A-BSD-U6-sgHPRT1
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a/hU6

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1 - TRC mTagBFP2-Synapsin1a

    Plasmid

    #191567

    Purpose

    Expresses an shRNA and mTagBFP2-synapsin1a

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc-sgRNA-Cas9.dora_1

    Plasmid

    #190700

    Purpose

    Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    dora (CG34401) sgRNA 1
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila U6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc-sgRNA-Cas9.dora_2

    Plasmid

    #190701

    Purpose

    Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    dora (CG34401) sgRNA 2
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila U6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc-sgRNA-Cas9.dora_3

    Plasmid

    #190702

    Purpose

    Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    dora (CG34401) sgRNA 3
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila U6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. px459-Pole4 KO sgRNA

    Plasmid

    #186935

    Purpose

    Pole4 KO in mouse ES cells

    Depositor

    Zhiguo Zhang

    Article

    Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

    Insert

    Pole4 KO sgRNA (Pole4 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge