We narrowed to 11,368 results for: Vars;

1. pEY38

   Plasmid

   #191042

   Purpose

   unc-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   unc-8 (unc-8 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEY13

   Plasmid

   #191019

   Purpose

   Fluorescent reporter driving amphid and phasmid nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   arrd-4 (ZK938.2 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEY20

   Plasmid

   #191026

   Purpose

   gcy-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   gcy-18 (gcy-18 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE Zeo IkB SR

   Plasmid

   #83023

   Purpose

   Retroviral expression of dominant negative IkB alpha

   Depositor

   Yi Zhang

   Article

   Kim et al Cell Rep. 2016 Sep 27;17(1):275-288. doi

   Insert

   NFKB Inhibitor Alpha (NFKBIA Human)
   

   Use

   Retroviral

   Mutation

   Serine 32 to Alanine, Serine 36 to Alanine

   Available Since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH29_32 series plasmid

   Plasmid

   #120901

   Purpose

   Also known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated device

   Depositor

   Domitilla Del Vecchio

   Article

   Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

   Inserts

   Ec-TTL-P109
   sRNA-A targeting sequence
   RBS TIR=974 of GFPop1 gene
   GFPop1
   RBS TIR=6474 of ECF32mut gene
   ECF32mut
   T7 terminator
   resource competitor
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pL0_EF-PtBle

   Plasmid

   #202180

   Purpose

   Level 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   ShBle expression cassette
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAd GFP HDAC5 WT

   Plasmid

   #32217

   Depositor

   Reuben Shaw

   Article

   Mihaylova et al Cell. 2011 May 13;145(4):607-21.

   Insert

   HDAC5 (HDAC5 Human)
   

   Use

   Adenoviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pAB088i

   Plasmid

   #79216

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-TnCAD-F3
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAB085c

   Plasmid

   #79208

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-FL
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (OR1); Ptet

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2-mCherry_SNAP_SNAPV_PACT_Dead

    Plasmid

    #173073

    Purpose

    Dead SNAP tag at the centrosome

    Depositor

    Heidi Hehnly

    Article

    Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

    Insert

    mCherry-SNAP-SNAP-Varied-PACT
    

    Tags

    mCherry-SNAP-SNAP-Varied

    Expression

    Mammalian

    Mutation

    AA144 in both SNAP tags changed from a cysteine t…

    Promoter

    CMV IE94

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. RT3GEP

    Plasmid

    #111164

    Purpose

    Tet-regulated miR-E (miR-30 variant)-based RNAi with tGFP reporter

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Retroviral

    Mutation

    WT

    Promoter

    T3G

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_SH3GLB1-1/1

    Plasmid

    #91346

    Purpose

    Protein expression and purification of human SH3 domain construct SH3GLB1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3GLB1-1/1 (SH3GLB1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. RT3REN

    Plasmid

    #111166

    Purpose

    Tet-regulated miR-E (miR-30 variant)-based RNAi with DsRed reporter

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Retroviral

    Mutation

    WT

    Promoter

    T3G

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-lxlplap

    Plasmid

    #45581

    Depositor

    Nirao Shah

    Article

    Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

    Inserts

    Green fluorescent protein
    Placental alkaline phosphatase (ALPP Human)
    

    Use

    Lentiviral

    Tags

    Histone 2B

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28 sf:mid.11

    Plasmid

    #191921

    Purpose

    Encodes a GFP design, sf:mid.11

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:mid.11
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Q69M Y145F T167I L220V V224I

    Promoter

    T7

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28 sf:mid.7

    Plasmid

    #191933

    Purpose

    Encodes a GFP design, sf:mid.7

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:mid.7
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72T Y145F V224I

    Promoter

    T7

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28 acid.6

    Plasmid

    #191912

    Purpose

    Encodes a GFP design, acid.6

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    acid.6
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Q69L S72A Y145M T167V H181L

    Promoter

    T7

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28 sf:acid.3

    Plasmid

    #191926

    Purpose

    Encodes a GFP design, sf:acid.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:acid.3
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S Q69L S72A T108V Y145M V224I

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28 mid.7

    Plasmid

    #191900

    Purpose

    Encodes a GFP design, mid.7

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.7
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72T Y145F V224I

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221 Cas9-POLD3

    Plasmid

    #183197

    Purpose

    Entry clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    Cas9-POLD3
    

    Use

    CRISPR; Gateway entry clone

    Mutation

    n/a

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pMXS-IRES-Blast SHMT1

    Plasmid

    #106304

    Purpose

    Express SHMT1

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    serine hydroxymethyltransferase 1 (SHMT1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pECFP-Rac1Y40C

    Plasmid

    #105293

    Purpose

    Control for FRET biosensor analysis - cannot bind downstream effectors

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    Rac1 (RAC1 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Y40C

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pPKm-202

    Plasmid

    #90492

    Purpose

    Expresses pcDNA3 - CMVmin - 5X UAS - pFR - CMVmin Luciferase

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    CMVmin Luciferase Fluc
    

    Expression

    Mammalian

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHH0103_RUSC1-1/1

    Plasmid

    #91379

    Purpose

    Protein expression and purification of human SH3 domain construct RUSC1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    RUSC1-1/1 (RUSC1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. sgRNA scaffold-spacer-human U6

    Plasmid

    #214697

    Purpose

    provide "sgRNA scaffold-spacer-human U6" fragment

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Insert

    sgRNA scaffold-spacer-human U6
    

    Use

    CRISPR

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mTFP1-H2A-10

    Plasmid

    #55488

    Purpose

    Localization: Nucleus/Histones, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    H2A (H2AC13 Human)
    

    Tags

    mTFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pHH0103_PACSIN2-1/1

    Plasmid

    #91330

    Purpose

    Protein expression and purification of human SH3 domain construct PACSIN2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PACSIN2-1/1 (PACSIN2 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEY22

    Plasmid

    #191028

    Purpose

    lim-4 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    lim-4 (lim-4 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEY17

    Plasmid

    #191023

    Purpose

    flp-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    flp-18 CELE_Y48D7A.2 (flp-18 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEY16

    Plasmid

    #191022

    Purpose

    flp-6 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    flp-6 (flp-6 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge