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We narrowed to 7,219 results for: cas9 plasmid

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Showing: 6351 - 6400 of 7219 results

  1. pEditA

    Plasmid
    #207531
    Purpose
    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Available Since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207531-plasmid-map-sequence-id-409606

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  3. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid
    #238007
    Purpose
    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.
    Depositor
    Simon Powell, Agnel Sfeir
    Article
    Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:
    Insert
    AAVS1 (AAVS1, PPP1R12C Human)
    Use
    Rna donor to study rt-dsbr
    Expression
    Mammalian
    Mutation
    3bp insertion signature (ATC) at Cas9 cut site in…
    Promoter
    CMV
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238007-plasmid-map-sequence-id-472649

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  4. Ring1-KO

    Plasmid
    #246224
    Purpose
    Knock out of Ring1
    Depositor
    Hengbin Wang
    Insert
    Ring1 gRNA (RING1 Human)
    Use
    Lentiviral
    Available Since
    Oct. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    246224-plasmid-map-sequence-id-487567

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  10. LLP618_αGCN4-KRAB-CO

    Plasmid
    #211782
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to transcriptional repressor KRAB, with GFP selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    aGCN4-mCherry
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect mCherr…
    Promoter
    pEF1a and pSV40
    Available Since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    211782-plasmid-map-sequence-id-417156

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  11. LLP617_αGCN4-mCherry

    Plasmid
    #211785
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to catalytically inert mCherry control, with GFP selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    SnoopCatcher-DNMT3A
    Tags
    2xOLLAS
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect DNMT3A…
    Promoter
    pEF1a and pSV40
    Available Since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211785-plasmid-map-sequence-id-417372

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  13. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

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  21. LLP790_αGCN4-EZH2-FL-CO

    Plasmid
    #211784
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to polycomb repressive complex subunit EZH2, with GFP selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    SnoopCatcher-KRAB
    Tags
    2xOLLAS
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect KRAB e…
    Promoter
    pEF1a and pSV40
    Available Since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211784-plasmid-map-sequence-id-417361

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  24. pET28b-BE3∆UGI

    Plasmid
    #96943
    Purpose
    The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).
    Depositor
    Daesik Kim
    Article
    Kim et al Nat Biotechnol. 2017 May;35(5):475-480.
    Insert
    BE3∆UGI
    Tags
    His6 tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    96943-plasmid-map-sequence-id-193667

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  25. pLV-U6-ZNF865-UbC-DsRed-P2A-Bsr

    Plasmid
    #241309
    Purpose
    Lentiviral SpCas9-gRNA (ZNF865) expression vector with DsRed-Express2-P2A-BlastR
    Depositor
    Robby Bowles
    Insert
    ZNF865 gRNA (ZNF865 Human)
    Use
    Lentiviral
    Available Since
    Dec. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    241309-plasmid-map-sequence-id-483062

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  26. pLR05_pGuide_B2M_sgRNA1

    Plasmid
    #131090
    Purpose
    sgRNA for SpCas9 mediated B2M KO
    Depositor
    Xiaojun Lian
    Insert
    B2M KO sgRNA 1 (B2M Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131090-plasmid-map-sequence-id-256909

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  27. pLR06_pGuide_B2M_sgRNA2

    Plasmid
    #131091
    Purpose
    sgRNA for SpCas9 mediated B2M KO
    Depositor
    Xiaojun Lian
    Insert
    B2M KO sgRNA 2 (B2M Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131091-plasmid-map-sequence-id-256596

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  30. pG3H-PE3max-attR1R2

    Plasmid
    #213049
    Purpose
    Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites
    Depositor
    Bing Yang
    Article
    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1
    Inserts
    Cas9 nickase
    M-MLV Reverse Transcriptase
    Use
    Note: this plasmid needs helper pvs1-vir2 for rep…
    Expression
    Plant
    Mutation
    H840A, R240K, N413K,
    Available Since
    Feb. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    213049-plasmid-map-sequence-id-422145

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  32. pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR

    Plasmid
    #154826
    Purpose
    Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)
    Depositor
    Lise Marie Grav
    Article
    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102
    Inserts
    mCherry
    NeoR
    ZsGreen1-DR
    Use
    CRISPR and Cre/Lox
    Tags
    PEST
    Expression
    Mammalian
    Promoter
    CMV, EF1a, and SV40 early promoter
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154826-plasmid-map-sequence-id-306204

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  34. pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR

    Plasmid
    #154827
    Purpose
    Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)
    Depositor
    Lise Marie Grav
    Article
    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102
    Inserts
    TagBFP
    HygR
    ZsGreen1-DR
    Use
    CRISPR and Cre/Lox
    Tags
    PEST
    Expression
    Mammalian
    Promoter
    CMV, EF1a, and SV40 early promoter
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154827-plasmid-map-sequence-id-306202

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  40. PE_CO-Mini-N

    Plasmid
    #190336
    Purpose
    Expressing the N-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, ngRNA and pegRNA for PCSK9 +1A insertion
    Depositor
    Rasmus Bak
    Article
    Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22
    Insert
    PE_CO-Mini-N, ngRNA and pegRNA for PCSK9 +1A insertion
    Use
    AAV
    Available Since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    190336-plasmid-map-sequence-id-374544

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  42. pUt-mNF-Cas13d

    Plasmid
    #224789
    Purpose
    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Use BlastR for positive selection and HSV-TK for negative selection.
    Depositor
    Gabor Balazsi
    Article
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Insert
    mCherry-P2A-RfxCas13d
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV-d2i
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    224789-plasmid-map-sequence-id-445954

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  43. px552-sg-gria1-HT

    Plasmid
    #187652
    Purpose
    Contains HaloTag to be inserted into the NTD of Gria1 (via HITI), single guide RNA to target Cas9 to Gria1 under control of the U6 promoter, and miRFP670 under control of human synapsin promoter.
    Depositor
    Erin O'Shea
    Article
    Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75
    Inserts
    HaloTag donor sequence
    miRFP670
    Use
    AAV and CRISPR
    Tags
    N/A
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    187652-plasmid-map-sequence-id-369120

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  45. pROS13_IDH2 #7

    Plasmid
    #166102
    Purpose
    This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.
    Depositor
    Matthias Heinemann
    Article
    Chen et al bioRxiv
    Insert
    IDH2 sgRNA #7 (IDH2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    166102-plasmid-map-sequence-id-330316

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  46. pROS13_IDH1 #4

    Plasmid
    #166101
    Purpose
    This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.
    Depositor
    Matthias Heinemann
    Article
    Chen et al bioRxiv
    Insert
    IDH1 sgRNA #4 (IDH1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    166101-plasmid-map-sequence-id-330298

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Showing: 6351 - 6400 of 7219 results