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pACE-GIRK4-S

Plasmid

#172425

Purpose

Expression of full-length, human GIRK4 with C-terminal StrepTag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK4 (KCNJ5 Human)

Tags

Streptag II

Expression

Insect

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEMS1725

Plasmid

#105870

Purpose

High copy number plasmid containing iCreERT2-FFrt-KanR-FFrt insert

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

iCreERT2-FFrt-KanR-FFrt

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+/N-SNAPf

Plasmid

#184415

Purpose

vector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

Depositor

Ann Miller

Article

Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

Type

Empty backbone

Use

For mrna synthesis

Tags

SNAPf-tag

Mutation

Codon optimized for expression in Xenopus laevis …

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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AEB-F12

Plasmid

#63849

Purpose

This pFTV1 vector contains a fluoride binding riboswitch, controlling the translation initaition rate of mRFP1 gene.

Depositor

Howard Salis

Article

Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

Insert

F12 fluoride riboswitch

Use

Synthetic Biology

Promoter

J23100

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pXTm-zebrafish Tspan4a

Plasmid

#210327

Purpose

Expresses Tetraspanin-4a (TSPAN4a) as migrasome marker

Depositor

Li Yu

Article

Jiang et al Nat Cell Biol. 2019 Aug;21(8):966-977. d

Insert

Tetraspanin 4a (tspan4a Zebrafish)

Use

Fish expression

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7xKS-mPlum

Plasmid

#117396

Purpose

Tn7 tagging vector pTn7xKS with mPlum expression scaffold

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

mPlum

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET-22HT-MSI2(8-193)

Plasmid

#60356

Purpose

Express the RBD of human MSI2 in bacterial cells for protein purification

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

human MSI2 (8-193) (MSI2 Human)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

contains amino acids 8-193 of MSI2

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pSLQ10714

Plasmid

#183959

Purpose

U6-crKlf4-CAG-hyperdCas12a-miniVPR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Inserts

HyperdCas12a
crRNA targeting Klf4

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D832A, D156R, E292R, D235R, D350R

Promoter

CAG and U6

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2-eab2-AA

Plasmid

#182153

Purpose

Expression of artificial intron separated mApple

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

AA

Use

Zebrafish expression

Promoter

eab2

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pFETCh_DNMT3B

Plasmid

#72365

Purpose

Donor vector for 3' FLAG tag of human DNMT3B

Depositor

Eric Mendenhall, Richard M. Myers

Insert

DNMT3B Homology Arms (DNMT3B Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pRG/III-hs-MBP-A2aTr316-H10

Plasmid

#121683

Purpose

Bacterial expression of adenosine A2a receptor fusion protein

Depositor

Reinhard Grisshammer, Richard Henderson

Article

Weiss et al Eur J Biochem. 2002 Jan;269(1):82-92.

Insert

adenosine A2a receptor (ADORA2A Human)

Tags

His10

Expression

Bacterial

Promoter

lac

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A23

Plasmid

#131968

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A23 (SLC25A23 Human)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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PX458_DNMT3B

Plasmid

#72366

Purpose

Encodes gRNA for 3' target of human DNMT3B along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Insert

DNMT3B (DNMT3B Human)

Use

CRISPR

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-TRAK2 [N390/43]

Plasmid

#190320

Purpose

Mammalian Expression Plasmid of anti-TRAK2 (Human). Derived from hybridoma N390/43.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TRAK2 (Homo sapiens) recombinant Mouse monoclonal antibody (TRAK2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pIC65

Plasmid

#63061

Purpose

Retroviral expression of EGFP-TEV-S-tag Mis12 for protein localization and affinity purification (LAP)

Depositor

Iain Cheeseman

Insert

hMis12, IMAGE clone 3351245 (MIS12 Human)

Use

Retroviral

Tags

EGFP and TEV-S

Expression

Mammalian

Mutation

L68R

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR_Hu_HEC1(WT)

Plasmid

#136350

Purpose

Human HEC1 cDNA in Gateway pENTR

Depositor

Guillermo de Cárcer

Insert

HEC1 (NDC80 Human)

Use

Gateway Cloning

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCSJ125

Plasmid

#104344

Purpose

Express yeast MDH2-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

Depositor

Alexander Varshavsky

Article

Chen et al Science. 2017 Jan 27;355(6323). pii: 355

Insert

MDH2

Tags

3xFLAG

Expression

Yeast

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIA4

Plasmid

#174034

Purpose

In vitro transcription of anti-CRISPR AcrIIA4 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIA4

Use

In vitro transcription

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIA2

Plasmid

#174033

Purpose

In vitro transcription of anti-CRISPR AcrIIA2 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIA2

Use

In vitro transcription

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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GMPR

Plasmid

#39147

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

GMPR (GMPR Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TFF1

Plasmid

#67047

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFF1 (TFF1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-bpNLS-Stab-exin21-eeBxb1-Stab (CJT607)

Plasmid

#248212

Purpose

bpNLS-, stabilon-, and exin21-tagged eeBxb1 recombinase, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-Stab-exin21-eeBxb1-Stab

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

eeBxb1(V74A, E229K, V375I)

Promoter

CMV and T7

Available Since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-EF1alpha-DEST-IRES-EGFP

Plasmid

#253770

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to EGFP by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

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pR6Ko1_Red

Plasmid

#243876

Purpose

GoldenBraid2.0 Cloning vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

mScarlet-I

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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RcP9

Plasmid

#242157

Purpose

Recombineering Plasmid. ITPG-induced expression of Recombitron operon (ccdB dropout cassette in Eco1 Retron); PSP-Tet promoter for Gene III induction upon M13 phage infection and aTc addition.

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-Nppa-Cre

Plasmid

#247326

Purpose

Expresses Cre recombinase under the control of the Nppa Promoter.

Depositor

William Pu, Mason Sweat

Article

Sweat et al Nat Cardiovasc Res. 2023 Oct;2(10):881-8

Insert

Nppa-Cre

Use

AAV

Expression

Mammalian

Mutation

TagRFP is out of frame and therefore not translat…

Promoter

Nppa Proximal promoter

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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APX1-pm-eGFP

Plasmid

#245520

Purpose

Plasmid for PiggyBac /ITR mediated insertion of pm-eGFP downstream of CAG promoter using Gateway recombination cassette

Depositor

Timothy Sanders, Kate Storey

Article

Dady et al Development. 2025 Aug 1:dev.205082. doi:

Insert

pm-eGFP

Expression

Mammalian

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gna15

Plasmid

#246003

Purpose

Express HA N-terminal tagged mouse Galpha15 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gna15 (Gna15 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-scFv-GCN4-sfGFP-IRESv4-HaloTag-tdMCP

Plasmid

#241137

Purpose

Expresses Anti-GCN4 scFv-sfGFP and HaloTag-tdMCP to track mature and nascent SunTag-tagged proteins and MS2 tagged mRNA

Depositor

Tim Stasevich

Article

Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

Inserts

Anti-GCN4 scFv
tdMCP

Tags

HaloTag and sfGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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MSI2_2xRRM_pGEX

Plasmid

#228800

Purpose

Recombinant expression of MSI2 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-2 (MSI2 Human)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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