We narrowed to 7,219 results for: cas9 plasmid
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Plasmid#208888PurposeAcinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).DepositorInsertdCas9, sgRNA expression cassette, sfGFP
UsePir dependent origin of replicationExpressionBacterialAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS263
Plasmid#122379PurposeCRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codonDepositorInsertHSP90
UseCRISPRExpressionYeastAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGE161
Plasmid#79453Purposerecipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, nptIIDepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE4
Plasmid#79443Purposerecipient plasmid [multiplexing]; pPcUbi:Cas9, BASTADepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE62
Plasmid#79444Purposerecipient plasmid [single nuclease]; p35S:Cas9, nptIIDepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE3
Plasmid#79442Purposerecipient plasmid [multiplexing]; pPcUbi:Cas9, nptIIDepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE64
Plasmid#79446Purposerecipient plasmid [single nuclease]; pPcUbi:Cas9, nptIIDepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE1
Plasmid#79440Purposerecipient plasmid [multiplexing]; p35S:Cas9, nptIIDepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE65
Plasmid#79447Purposerecipient plasmid [single nuclease]; pPcUbi:Cas9, BASTADepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE146
Plasmid#79449Purposerecipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, BASTADepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE167
Plasmid#79457Purposerecipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, HygroDepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE63
Plasmid#79445Purposerecipient plasmid [single nuclease]; p35S:Cas9, BASTADepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE144
Plasmid#79448Purposerecipient plasmid [multiplexing]; p35S:Cas9, FAST, BASTADepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE160
Plasmid#79452Purposerecipient plasmid [multiplexing]; p35S:Cas9, FAST, nptIIDepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE166
Plasmid#79456Purposerecipient plasmid [multiplexing]; p35S:Cas9, FAST, HygroDepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE2
Plasmid#79441Purposerecipient plasmid [multiplexing]; p35S:Cas9, BASTADepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330-LMNA-gRNA1
Plasmid#122507Purposeexpresses WT spCas9 and a chimeric gRNA targeting human LMNADepositorInsertLMNA-gRNA1
UseCRISPRExpressionMammalianPromoterU6Available SinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJP_Osc05
Plasmid#229040PurposeLight inducible three-node circuit that can generate ring patterns in a growing bacterial colony upon blue light pulses. Heterologous expression of dCas9, Csy4, and VVD-AraC are necessary.DepositorInsertpBAD promoter
ExpressionBacterialAvailable SinceSept. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAT15489_dABE8e
Plasmid#174127Purposeplasmid expressing ABE8e with dCas9DepositorInsertdABE8e
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianPromoterEF1-aAvailable SinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
hSyn-IRES2-GFP
Plasmid#234875PurposeLentiviral plasmid with a multiple cloning site followed by IRES2-GFP.DepositorInsertEGFP
UseLentiviralExpressionMammalianPromoterhSYnAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_eGFP_191
Plasmid#111821PurposeKnockout eGFPDepositorInsertgRNA targeting eGFP 171-191
UseCRISPRTagsmCherryExpressionBacterial and MammalianPromoterU6Available SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_eGFP_369
Plasmid#111823PurposeKnockout eGFPDepositorInsertgRNA targeting eGFP 349-369
UseCRISPRTagsmCherryExpressionBacterial and MammalianPromoterU6Available SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_eGFP_314
Plasmid#111822PurposeKnockout eGFPDepositorInsertgRNA targeting eGFP 294-314
UseCRISPRTagsmCherryExpressionBacterial and MammalianPromoterU6Available SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-NucGFP
Plasmid#184696PurposeLentiviral transfer plasmid for expression of NucGFPDepositorInsertNucGFP
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT9749-dABE
Plasmid#162998Purposeplasmid expressing ABE with dCas9DepositorInsertdABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianPromoterEF1-aAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-ArgSen
Plasmid#184691PurposeLentiviral transfer plasmid for expression of ArgSenDepositorInsertArgSen
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLY86
Plasmid#130949PurposeEngineered sgRNA-LEA2-WTmis2 generator circuit including promoter Plux2, 2 BoxB aptamers and 2 mis-matches in sgRNA scaffoldDepositorInsertsgRNA-LEA2-WTmis2
UseSynthetic BiologyExpressionBacterialPromoterPlux2Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVP144
Plasmid#222081PurposeSingle component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and eforRED for plasmid eviction.DepositorInsertdCas9-PmCDA1, sfGFP, sacB and eforRED
UseCRISPRExpressionBacterialAvailable SinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVP143
Plasmid#222080PurposeSingle component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and AeBlue for plasmid eviction.DepositorInsertdCas9-PmCDA1, sfGFP, sacB and AeBlue
UseCRISPRExpressionBacterialAvailable SinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGFP-CUG-IRES-mCherry
Plasmid#222110PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralMutationCUG start codonAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISP_IS
Plasmid#120425PurposeThis plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.DepositorInsertCRISPR spacers targeting IS1, IS5, IS3
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterconstitutiveAvailable SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGFP-AUA-IRES-mCherry
Plasmid#222116PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralMutationAUA start codonAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-AGC-IRES-mCherry
Plasmid#222119PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralMutationAGC start codonAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ACG-IRES-mCherry
Plasmid#222113PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralMutationACG start codonAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-UUG-IRES-mCherry
Plasmid#222112PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralMutationUUG start codonAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-AUU-IRES-mCherry
Plasmid#222118PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralMutationAUU start codonAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-AGG-IRES-mCherry
Plasmid#222115PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralMutationAGG start codonAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-AUC-IRES-mCherry
Plasmid#222117PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralMutationAUC start codonAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCyCBE
Plasmid#226505PurposeA cytidine deaminase-mediated base-editing plasmid in cyanobacteriaDepositorInsertsAPOBEC1-Cas9D10A
sgRNA
sacB gene
UseCRISPRAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLY185
Plasmid#184146PurposeRFP generator controlled by Plux2 promoterDepositorInsertrfp
UseSynthetic BiologyPromoterPlux2Available SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti-mTagBFP2
Plasmid#216126PurposeLentiviral vector for expressing mTagBFP2.DepositorInsertEF1a-mTagBFP2-WPRE
UseLentiviralExpressionMammalianAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
NPM1_mNeonGreen_Donor
Plasmid#178093PurposeHDR donor plasmid to tag NPM1 with mNeonGreen using eSpCas9(1.1)_No_FLAG_NPM1_G6.DepositorInsertNPM1-mNeonGreen HDR donor
UseCRISPRExpressionMammalianAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
LMNA_mScarlet-I_Donor
Plasmid#178092PurposeHDR donor plasmid to tag LMNA with mScarlet-I using eSpCas9(1.1)_No_FLAG_LMNA_G2.DepositorInsertLMNA-mScarlet-I HDR donor
UseCRISPRExpressionMammalianAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-GFP ArgSen
Plasmid#184695PurposeLentiviral transfer plasmid for expression of GFP ArgSenDepositorInsertGFP ArgSen
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-GFP RanSen
Plasmid#184697PurposeLentiviral transfer plasmid for expression of GFP RanSenDepositorInsertGFP RanSen
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-ArgSen (-)NLS
Plasmid#184689PurposeLentiviral transfer plasmid for expression of ArgSen (-)NLSDepositorInsertArgSen (-)NLS
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-P2A-hMLH1dn
Plasmid#222997PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)DepositorInsertPE7-P2A-hMLH1dn
TagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDeletion of residues 754-756 in MLH1 as described…PromoterCMVAvailable SinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJEC819
Plasmid#190815PurposeCRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.DepositorInsertdCas9-XTEN-rpoA
UseCRISPR and Synthetic BiologyTagsXTEN (linker), followed by the N-terminal domain …PromoterSP30Available SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRIS
Plasmid#120424PurposeThis plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.DepositorInsertCRISPR spacers targeting IS1, IS5, IS3, IS150
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterconstitutiveAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV8-cPE-N
Plasmid#183538PurposeDeliver a split compact Prime Editor in vivoDepositorInsertN-terminal fragment of SpCas9 nickase (H840A)
UseAAVExpressionMammalianAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only