We narrowed to 9,185 results for: Pol;

1. pKM136

   Plasmid

   #134177

   Purpose

   Bacterial expression of his-CENP-C 700-943

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

   Insert

   his-CENP-C 700-943 (CENPC Human)
   

   Expression

   Bacterial

   Mutation

   aa 700-943

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR207-OEP7(1–50)

   Plasmid

   #100601

   Purpose

   Gateway entry vector for AtOEP7(1–50)

   Depositor

   Yutaka Kodama

   Article

   Tanaka et al J Plant Res. 2017 Jun 20. doi: 10.1007/s

   Insert

   OEP7(1–50) (OEP7 Mustard Weed)
   

   Use

   Gateway entry vector

   Available Since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pst39-NST-clone14

   Plasmid

   #133421

   Purpose

   S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).

   Depositor

   Gerhard Wagner

   Insert

   NIP1/SUI1/TIF5 (NIP1, TIF5, SUI1 Budding Yeast)
   

   Expression

   Bacterial

   Mutation

   ORF for Nip1 encodes aa 4-156 (not complete ORF),…

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFA-dabAB2

   Plasmid

   #133012

   Purpose

   pFA carrying the badabA2 (locus_tag: BAS2958) and badabB2 genes (locus_tag: BAS2959)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Expression

   Bacterial

   Promoter

   tetR

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFE-dabAB2 C351A

   Plasmid

   #133005

   Purpose

   pFE carrying a dabA2 gene with a C351A mutation and a dabB2 gene (Uniprot: D0KWS8)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Expression

   Bacterial

   Mutation

   C351A

   Promoter

   tetR

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. YCe1885 HC_Kan_Kozak-ATG-MLS_p6

   Plasmid

   #100676

   Purpose

   part designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targeting

   Depositor

   Yizhi Cai

   Article

   Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

   Insert

   Kozak-ATG-MLS
   

   Use

   Synthetic Biology

   Available Since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. YCe2735 HC_Kan_Kozak-ATG-IgkL_p6

   Plasmid

   #100697

   Purpose

   part designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targeting

   Depositor

   Yizhi Cai

   Article

   Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

   Insert

   Kozak_ATG_IgkL
   

   Use

   Synthetic Biology

   Available Since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CTL2 (M2225R)

   Plasmid

   #96952

   Purpose

   Expresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coli

   Depositor

   Douglas Rees

   Article

   Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

   Insert

   CTL2 (M222R) of PIEZO1
   

   Tags

   6x Histidine Tag

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21a - MPX

   Plasmid

   #89619

   Purpose

   [Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Mitocondrial Tryparedoxin peroxidase
   

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21a - EtfA

    Plasmid

    #89616

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Electron transport flavoprotein alpha subunit
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GST-PC-1-CTS

    Plasmid

    #86067

    Purpose

    A bacterial expression plasmid encoding polycystin-1 ciliary targeting signal with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Polycystin-1 (PKD1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains the region from 4297-4303

    Promoter

    tac

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21a - TDR

    Plasmid

    #89627

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi thiol dependent reductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    thiol dependent reductase
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21a - NFDD

    Plasmid

    #89624

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    NAD/FAD dependent dehydrogenase
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBP-J23108

    Plasmid

    #72964

    Purpose

    Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    J23108 promoter
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBP-J23114

    Plasmid

    #72965

    Purpose

    Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    J23114 promoter
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBP-T7_RBS

    Plasmid

    #72978

    Purpose

    T7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    T7+RBS
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTU2-A-lacZ

    Plasmid

    #72950

    Purpose

    Level 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - A site
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTU2-B-lacZ

    Plasmid

    #72951

    Purpose

    Level 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - B site
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTU2-C-lacZ

    Plasmid

    #72952

    Purpose

    Level 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - C site
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTU2-D-lacZ

    Plasmid

    #72953

    Purpose

    Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - D site
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTU2-B-RFP

    Plasmid

    #72955

    Purpose

    Level 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - B site
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTU2-C-RFP

    Plasmid

    #72956

    Purpose

    Level 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - C site
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTU2-D-RFP

    Plasmid

    #72957

    Purpose

    Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - D site
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTU2-b-RFP

    Plasmid

    #72959

    Purpose

    Level 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABC

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - b site
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

25. CAG-mCherry-RORbshRNA2

    Plasmid

    #73984

    Purpose

    RORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse RORb gene
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CAG-EGFP-RORbshRNA1

    Plasmid

    #73985

    Purpose

    RORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse RORb gene
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CAG-EGFP-RORbshRNA2

    Plasmid

    #73986

    Purpose

    RORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of EGFP.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse RORb gene
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CAG-mCherry-Otx2shRNA2

    Plasmid

    #73980

    Purpose

    Otx2 shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse Otx2 gene
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCellFree_G03 ZFYVE9

    Plasmid

    #67134

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    ZFYVE9 (ZFYVE9 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Mutation

    F1359I

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pCGS652

    Plasmid

    #62220

    Purpose

    Mammalian expression TALEN vector with N152, C+18 truncations

    Depositor

    Daniel Voytas

    Article

    Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

    Type

    Empty backbone

    Use

    TALEN

    Tags

    AcV5 and SV40 NLS

    Expression

    Mammalian

    Promoter

    miniCaggs

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge