We narrowed to 9,185 results for: Pol;

1. pZac2.1-GfaABC1D-Rpl22-HA

   Plasmid

   #111811

   Purpose

   Express Rpl22HA in astrocytes

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Insert

   Ribosomal protein L22 (Rpl22 Mouse)
   

   Tags

   HA

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-Ftractin-mCherry

   Plasmid

   #85131

   Purpose

   lentiviral expression of an F-actin live-cell marker (mCherry)

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)
   

   Use

   Lentiviral

   Tags

   mCherry

   Promoter

   EF1a

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGM265

   Plasmid

   #220179

   Purpose

   Expresses exogenous wild-type TTC1 with an N-terminal sfGFP tag

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   TTC1 (TTC1 Human)
   

   Use

   Lentiviral

   Tags

   sfGFP

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCas9-GFP

   Plasmid

   #78546

   Purpose

   Fusion protein Cas9+GFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Fusion protein with Cas9

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLyGo-Ec-8

   Plasmid

   #163136

   Purpose

   pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   P(rhaB)

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCas9-T2A-GFP

   Plasmid

   #78548

   Purpose

   Cas9 protein with GFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   T2A-GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   In frame with Cas9

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-SYN-sDarken

   Plasmid

   #184796

   Purpose

   Serotonin Sensor sDarken under the control of a hSyn Promoter.

   Depositor

   Olivia Masseck

   Article

   Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

   Insert

   sDarken
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TEV-HRV-3C Proteases-mCherry

   Plasmid

   #243795

   Purpose

   Encodes for mammalian expression of TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

   Depositor

   Cole DeForest

   Article

   Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

   Insert

   TEV and HRV-3C proteases
   

   Expression

   Mammalian

   Available Since

   Oct. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. LCC-ICCG_pET21b

   Plasmid

   #215408

   Purpose

   Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   LCC-ICCG
   

   Expression

   Bacterial

   Mutation

   F243I/D238C/S283C/Y127G

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCas9-BFP

    Plasmid

    #78545

    Purpose

    Fusion protein Cas9+BFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Fusion protein with Cas9

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFW2500

    Plasmid

    #224213

    Purpose

    Bacteroides genomic editing tool; IPTG inducible-Fncpf1 and recT

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Insert

    Fncpf1 and recT
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOPINB-AMSH*

    Plasmid

    #66712

    Purpose

    Expresses activated AMSH in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Inserts

    hbp (Stam2 Mouse)
    AMSH (STAMBP Human)
    

    Tags

    His-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pPRIME-CMV-LNGFR-FF3

    Plasmid

    #11666

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of LNGFR and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    LNGFR

    Expression

    Mammalian

    Available Since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS_3E5E_luciferase

    Plasmid

    #103055

    Purpose

    Encodes Ebola virus minigenome with firefly luciferase reporter gene. Minigenome is under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    Ebolavirus minigenome
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM6-RgTALsyn

    Plasmid

    #100947

    Purpose

    Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    T7

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPINB-OTUB1*

    Plasmid

    #65441

    Purpose

    Expresses activated OTUB1 in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Inserts

    UbcH5b (UBE2D2 Human)
    otubain-1 (OTUB1 )
    

    Tags

    His-3C

    Expression

    Bacterial

    Mutation

    Active site cysteine mutated to alanine

    Promoter

    T7

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pGEX4T3-GST-Tev

    Plasmid

    #177142

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of the Tev protease

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    Tev
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-N1 human cofilin WT

    Plasmid

    #50859

    Purpose

    expresses human cofilin fused to EGFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 (CFL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-CAG-empty-ires-EGFP

    Plasmid

    #122953

    Purpose

    Lentiviral expression empty vector under CAG promoter with EGFP expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiCCAT1-Red

    Plasmid

    #226521

    Purpose

    CCAT1 transgene with fluorescent protein mKate2

    Depositor

    Neville Sanjana

    Article

    Caragine et al Nat Commun. 2025 Feb 13;16(1):1611. doi:

    Insert

    EF1a_mKate2_CMV_CCAT1 (CCAT1 Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pREP3-TEV-NLS

    Plasmid

    #21146

    Depositor

    Stephen Kearsey

    Article

    Yang et al BMC Mol Biol. 2005 Jun 7;6:13. doi: 10.1

    Insert

    TEV protease (TEVgp1 tobacco etch virus)
    

    Use

    S. pombe

    Tags

    2xNLS, NLS, and myc

    Expression

    Yeast

    Available Since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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22. pN1-CMV-H-sDarken

    Plasmid

    #184800

    Purpose

    High affinity version of the Serotonin Sensor sDarken under the control of a CMV Promoter.

    Depositor

    Olivia Masseck

    Article

    Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

    Insert

    H-sDarken
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLentiCas9-T2A-BFP

    Plasmid

    #78547

    Purpose

    Cas9 protein with BFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    T2A-BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    In frame with Cas9

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLyGo-Kp-1

    Plasmid

    #163143

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pBa.Halo-LDLR

    Plasmid

    #162717

    Purpose

    Mammalian expression of human LDLR

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    LDLR (LDLR Human)
    

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Chicken Beta actin

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLyGo-Ec-1

    Plasmid

    #163127

    Purpose

    pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Ubiquitin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGL2-NOS2Promoter-Luciferase

    Plasmid

    #19296

    Depositor

    Charles Lowenstein

    Article

    Lowenstein et al Proc Natl Acad Sci U S A. 1993 Oct 15. 9

    Insert

    NOS2 Promoter (Nos2 Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Nov. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pZH81 pET21b Rat1-His

    Plasmid

    #231650

    Purpose

    Bacterial expression vector expressing yeast S. cerevisiae rat1 with a C-terminal his tag

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    Rat1 (RAT1 Budding Yeast)
    

    Tags

    6His

    Expression

    Bacterial

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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29. pBP-T7_RBS-His6-Thrombin

    Plasmid

    #72979

    Purpose

    T7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    T7+RBS+His6+thrombin
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pLyGo-Bs-1

    Plasmid

    #163137

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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