We narrowed to 9,185 results for: Pol;

1. pLJM1-Flag-Rheb

   Plasmid

   #165031

   Purpose

   expression of Flag tagged Rheb protein in mammalian cells

   Depositor

   Ken Inoki

   Article

   Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

   Insert

   Rheb (RHEB Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET32a-TRXtag

   Plasmid

   #11516

   Depositor

   Pamela Bjorkman

   Article

   Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

   Insert

   None - thioredoxin is vector encoded (trxA )
   

   Tags

   His, His (out of frame), and Trx

   Expression

   Bacterial

   Mutation

   vector modified; removed N-terminal His tag by di…

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

3. p21 promoter A

   Plasmid

   #16462

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

   Insert

   p21 promoter (CDKN1A Human)
   

   Expression

   Mammalian

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV::TVA/tRFP

   Plasmid

   #164486

   Purpose

   Dual CMV promoter plasmid to express TVA and TurboRFP

   Depositor

   Hollis Cline

   Article

   Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

   Inserts

   TVA (CD320 Chicken)
   TurboRFP
   

   Expression

   Mammalian

   Promoter

   sCMV and sCMV IE94

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. gRNA-ura-HYB

   Plasmid

   #64330

   Purpose

   ura3 deletion gRNA cassette carried by pRS42H

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   gBlock product of ura3 deletion gRNA cassette
   

   Expression

   Yeast

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pMALcPP-MS2cp

   Plasmid

   #203351

   Purpose

   Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria

   Depositor

   Thomas Cech

   Article

   Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;12

   Insert

   MS2 coat protein
   

   Tags

   Hx6-MBP

   Expression

   Bacterial

   Mutation

   V75E and A81G, to prevent capsid assembly

   Available Since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti CMVie-IRES-BlastR

   Plasmid

   #119863

   Purpose

   3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection

   Depositor

   Ghassan Mouneimne

   Article

   Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV immediate early

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cut190*_pET21b

   Plasmid

   #215404

   Purpose

   Expression construct for Cut190* gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   Cut190*
   

   Expression

   Bacterial

   Mutation

   S226P/R228S

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-EGFP-IRES-Neo

   Plasmid

   #128660

   Purpose

   mammalian expression of EGFP with neomycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Wild type

   Promoter

   CMV

   Available Since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmRFP-N1 human cofilin WT

    Plasmid

    #50856

    Purpose

    expresses human cofilin fused to mRFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 (CFL1 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZac2.1-GfaABC1D-GAT3-HA-mCherry

    Plasmid

    #117722

    Purpose

    Expresses a GABA transporter GAT-3 (Slc6a11)

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Insert

    Slc6a11 (Slc6a11 Mouse)
    

    Use

    AAV

    Tags

    HA and mCherry

    Expression

    Mammalian

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBetaActin-mNeonGreen-CAMSAP3

    Plasmid

    #191322

    Purpose

    Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    mNeonGreen-CAMSAP3 (Camsap3 Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDARMO_CMVT_3xFLAG_BIRC6

    Plasmid

    #197967

    Purpose

    Mammalian cell expression of wt 3xFLAG-BIRC6

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    BIRC6 (BIRC6 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    L1332V

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-N1 Raptor Halo

    Plasmid

    #112754

    Purpose

    express Raptor-Halo

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Raptor-Halo (RPTOR Human)
    

    Tags

    Halo

    Expression

    Mammalian

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV Puro SCRIB WT

    Plasmid

    #88886

    Purpose

    SCRIB WT in MSCV backbone

    Depositor

    Senthil Muthuswamy

    Article

    Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

    Insert

    SCRIB (SCRIB Human)
    

    Use

    Retroviral

    Tags

    N/A

    Mutation

    N/A

    Promoter

    MSCV promoter

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYES1L-URA

    Plasmid

    #84301

    Purpose

    E.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.

    Depositor

    George Church

    Article

    Ostrov et al Science. 2016 Aug 19;353(6301):819-22. d

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBluescriptII[attB/Ins1]

    Plasmid

    #74100

    Purpose

    "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

    Depositor

    Ben Szaro

    Article

    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

    Inserts

    Phi C31 attb element
    Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
    

    Expression

    Bacterial

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-ATXN1

    Plasmid

    #48189

    Purpose

    Expresses Ataxin1

    Depositor

    Huda Zoghbi

    Article

    Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

    Insert

    Ataxin1 (ATXN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMVTAG-NEMO

    Plasmid

    #11970

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    NEMO (IKBKG Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST_CMV_N_Flag_BIRC6

    Plasmid

    #197973

    Purpose

    Mammalian cell expression of wt FLAG-BIRC6

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    BIRC6 (BIRC6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L1332V

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCB163

    Plasmid

    #37388

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

    Insert

    Dynactin 2 (DCTN2 Human)
    

    Use

    Retroviral

    Tags

    GFP, S-tag, and TEV

    Expression

    Mammalian

    Mutation

    A7P

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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22. sAB-K29

    Plasmid

    #204735

    Purpose

    synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin

    Depositor

    Minglei Zhao

    Article

    Yu et al Nat Chem Biol. 2021 Aug;17(8):896-905. d

    Insert

    synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin
    

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. 7Gli:GFP

    Plasmid

    #110494

    Purpose

    reporter of Hedgehog signaling activity

    Depositor

    Michael Lewis

    Article

    Li et al PLoS Genet. 2016 May 19;12(5):e1006054.

    Insert

    7 repeats of the Gli binding site GAACACCCA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    7 repeats of Gli biding site

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. PB-fLuc=tdT

    Plasmid

    #120870

    Purpose

    Piggybac transposon plasmid for live animal optical imaging

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    pcDNA3-fLuc=tdT
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. CMV BARK p2A mCherry

    Plasmid

    #117689

    Purpose

    CMV-iBARK-p2A-mCherry: Mammalian expression vector for Gaq silencing

    Depositor

    Baljit Khakh

    Article

    Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

    Insert

    BARKrgs peptide
    

    Tags

    HA / FLAG

    Expression

    Mammalian

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pDEST26-USP47

    Plasmid

    #64326

    Purpose

    Mammalian expression and purification of USP47.

    Depositor

    Grigory Dianov

    Article

    Parsons et al Mol Cell. 2011 Mar 4;41(5):609-15. doi:

    Insert

    USP47 (USP47 Human)
    

    Tags

    His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. pIC291

    Plasmid

    #51403

    Purpose

    Retroviral expression of EGFP-TEV-S-tag SKA1 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Article

    Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

    Insert

    Ska1 IMAGE 3909951 (SKA1 Human)
    

    Use

    Retroviral

    Tags

    EGFP, S tag, and TEV protease site

    Expression

    Mammalian

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PB-Aka-P2A-GFP

    Plasmid

    #120867

    Purpose

    Piggybac transposon plasmid for live animal optical imaging

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    Akaluc
    

    Expression

    Mammalian

    Mutation

    insert Akaluc and a P2A site

    Promoter

    CAG Promoter

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. gRNA-trp-HYB

    Plasmid

    #64331

    Purpose

    trp1 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of trp1 deletion gRNA cassette
    

    Expression

    Yeast

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro

    Plasmid

    #129537

    Purpose

    PiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.

    Depositor

    Hideyuki Okano

    Article

    Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

    Insert

    tetR-3xFlag-HA-2A-Puro
    

    Use

    Synthetic Biology

    Promoter

    hPGK promoter

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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