We narrowed to 9,185 results for: Pol;

1. pVETL-Cmcs-Flag-Ataxin3Q25

   Plasmid

   #22118

   Depositor

   Henry Paulson

   Article

   Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pGBW-m4046757

   Plasmid

   #145657

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLVX Flag RagC WT

   Plasmid

   #112756

   Purpose

   express Flag-RagC

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Flag-RagC (RRAGC Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. -227 hTF

   Plasmid

   #15442

   Depositor

   Nigel Mackman

   Article

   Oeth et al Arterioscler Thromb Vasc Biol. 1997 Feb

   Insert

   Tissue factor promoter (F3 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   July 27, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pCellFree_G03 GDI2

   Plasmid

   #67093

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   GDI2 (GDI2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pFA-DAB2

   Plasmid

   #162690

   Purpose

   Expresses DabAB2 inorganic carbon transport complex

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   DabAB2 inorganic carbon transport complex
   

   Expression

   Bacterial

   Promoter

   PLtet0-1 promoter

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti CMVie-IRES-BlastR alt MCS

   Plasmid

   #120862

   Purpose

   3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection

   Depositor

   Ghassan Mouneimne

   Article

   Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV immediate early

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVenus-N-SLC35B2

   Plasmid

   #155038

   Purpose

   Transient expression of SLC35B2-Venus

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   SLC35B2 (SLC35B2 Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   CMV

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTRY-YFP

   Plasmid

   #15302

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   YFP
   

   Use

   Gateway entry

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pbGH

    Plasmid

    #118061

    Purpose

    GBPart - Bovine growth hormone terminator

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    bgH_poly(A)
    

    Use

    Synthetic Biology; Gbpart

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIC306

    Plasmid

    #52257

    Purpose

    Retroviral expression of EGFP-TEV-S-tag SKA3 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Article

    Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

    Insert

    Ska3 (SKA3 Human)
    

    Use

    Retroviral

    Tags

    EGFP, S tag, and TEV protease site

    Expression

    Mammalian

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIA1363

    Plasmid

    #166861

    Purpose

    Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp8 (ORF1ab Synthetic)
    

    Tags

    His-GB1

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIA1362

    Plasmid

    #166860

    Purpose

    Expression of codon optimized SARS-COV-2 Nsp7 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp7 (ORF1ab Synthetic)
    

    Tags

    His-GB1

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. mVenus-PKCB1 K371R

    Plasmid

    #195230

    Purpose

    Retroviral vector containing GFP tagged PKCB1. Kinase inactive mutant.

    Depositor

    David Bryant, Keith Mostov

    Article

    Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

    Insert

    PKCB1 (PRKCB Human)
    

    Use

    Retroviral

    Mutation

    K371R

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBetaActin-Halo-CAMSAP3

    Plasmid

    #191329

    Purpose

    Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    Halo-CAMSAP3 (Camsap3 Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. NylB'-SCY_pET21b

    Plasmid

    #215417

    Purpose

    Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB'-SCY
    

    Expression

    Bacterial

    Mutation

    R187S/F264C/D370Y

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. CAG-Otx2-IRES-GFPd2

    Plasmid

    #73996

    Purpose

    Plasmid expressing Otx2(isoform b)-IRES-GFPd2

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    Otx2(isoform b)-Ires-GFP (Otx2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 human cofilin S3A

    Plasmid

    #50854

    Purpose

    expresses constituvely active cofilin in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 S3A (CFL1 Human)
    

    Expression

    Mammalian

    Mutation

    constitutively active, S3A

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pET28a-CohIII-CBM-HIS-LPETGG

    Plasmid

    #91697

    Purpose

    CohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    CohIII-CBM-HIS-LPETGG
    

    Tags

    HIS-Tag and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Cysteine deletion in CBM C63S

    Promoter

    T7

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3XFlag DVL2 (∂DIX)

    Plasmid

    #24805

    Depositor

    Jeff Wrana

    Article

    Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

    Insert

    DVL2 (DVL2 Human)
    

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Deletion of the N-terminal DIX domain

    Available Since

    May 14, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

21. PB-Antares2

    Plasmid

    #120868

    Purpose

    Piggybac transposon plasmid for live animal optical imaging

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    pcDNA3-Antares2
    

    Expression

    Mammalian

    Mutation

    Antares2 amplicon

    Promoter

    CAG Promoter

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCB169

    Plasmid

    #37390

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

    Insert

    dynein light chain TcTex-type 3 (DYNLT3 Human)
    

    Use

    Retroviral

    Tags

    GFP, S-tag, and TEV

    Expression

    Mammalian

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCellFree_G03 IL1B

    Plasmid

    #67066

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    IL1B (IL1B Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

24. 3XFlag DVL2 (∂361-736)

    Plasmid

    #24806

    Depositor

    Jeff Wrana

    Article

    Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

    Insert

    DVL2 (DVL2 Human)
    

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Deletion of aa 361-736

    Available Since

    May 14, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

25. pBS hIhh (CT#402)

    Plasmid

    #13995

    Depositor

    Cliff Tabin

    Article

    Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

    Insert

    Ihh (IHH Human)
    

    Expression

    Bacterial

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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26. C204

    Plasmid

    #174358

    Purpose

    For expressing siRNA-resistant human PABPC1 in human cell lines via transfection

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    PABPC1 (PABPC1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Multiple silent mutations introduced to be resist…

    Available Since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pIC292

    Plasmid

    #51405

    Purpose

    Retroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Article

    Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

    Insert

    Ska2 IMAGE 4685972 (SKA2 Human)
    

    Use

    Retroviral

    Tags

    EGFP, S tag, and TEV protease site

    Expression

    Mammalian

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDARMO_CMVT_3xFLAG_BIRC6_BIRmut

    Plasmid

    #197968

    Purpose

    Mammalian cell expression of 3xFLAG-BIRC6_BIRmut

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    BIRC6 (BIRC6 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    C328S, C331S, L1332V

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pN1-CMV-L-sDarken

    Plasmid

    #184801

    Purpose

    Low affinity version of the Serotonin Sensor sDarken under the control of a CMV Promoter.

    Depositor

    Olivia Masseck

    Article

    Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

    Insert

    L-sDarken
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. WNV NS2B-NS3 protease (K104A - catalytically active, don't self cleave); aliases: West Nile Virus NS2B-NS3 fusion protein

    Plasmid

    #204794

    Purpose

    Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    West Nile NS2B-NS3 fusion
    

    Expression

    Bacterial

    Available Since

    Aug. 29, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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