We narrowed to 9,185 results for: Pol;

1. pGEX-NEMOD311N

   Plasmid

   #11968

   Depositor

   Jon Ashwell

   Article

   Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

   Insert

   NEMOD311N (IKBKG Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Asp311 to Asn

   Available Since

   July 13, 2006

   Availability

   Academic Institutions and Nonprofits only

2. ch-Tog

   Plasmid

   #28290

   Depositor

   Linda Wordeman

   Article

   Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

   Insert

   ch-TOG (CKAP5 Human)
   

   Expression

   Bacterial and Mammalian

   Mutation

   K19R, E118G

   Available Since

   April 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV.PARP1#3

   Plasmid

   #14546

   Depositor

   Didier Trono

   Article

   Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

   Insert

   PARP1 shRNA (PARP1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   March 27, 2007

   Availability

   Academic Institutions and Nonprofits only

4. GAL FLAG25Q+ProGFPp303

   Plasmid

   #15572

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 25Q + Pro (HTT Human)
   

   Tags

   FLAG and GFP

   Expression

   Yeast

   Mutation

   Proline-rich region present

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCellFree_G03 ATF1

   Plasmid

   #67065

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   ATF1 (ATF1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pENV8(GAAA)-GFPuv

   Plasmid

   #70128

   Purpose

   Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop

   Depositor

   Robert Batey

   Article

   Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

   Insert

   kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-EF1A-LivePAR(R163A)-Hygro

   Plasmid

   #187609

   Purpose

   EGFP fused to the C-terminus of a WWE domain containing the mutation Arg163Ala & a hygromycin resistance cassette.

   Depositor

   Robert Sobol

   Article

   Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

   Insert

   LivePAR(R163A)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R163A mutation in the WWE Domain

   Promoter

   EF1A

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCellFree_G03 ANXA1

   Plasmid

   #67071

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   ANXA1 (ANXA1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pDONR221 Cas9-RFC5

   Plasmid

   #183200

   Purpose

   Entry clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   Cas9-RFC5
   

   Use

   CRISPR; Gateway entry clone

   Mutation

   n/a

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCellFree_G03 STAT3

    Plasmid

    #67120

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pSL1180-HR-PUbEGFP-NLS

    Plasmid

    #176681

    Purpose

    Expression of nuclear localized EGFP under Ae. aegypti Polyubiquitin promoter (AAEL003888), EGFP version of pSL1180-HR-PUbECFP (Addgene #47917)

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    EGFP
    

    Expression

    Insect

    Promoter

    Ae. aegypti poly-ubiquitin (AAEL003888)

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWL502-crRNA

    Plasmid

    #174383

    Purpose

    The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei

    Depositor

    Jing Han

    Article

    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

    Insert

    a crRNA targeting the template strand
    

    Use

    CRISPR; Archaeal expression

    Expression

    Bacterial

    Promoter

    PhaR promoter

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCellFree_G03 ISG15

    Plasmid

    #67054

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pTU2S-b (colE1 origin)

    Plasmid

    #74092

    Purpose

    Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Golden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    None

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV5-Flag-RhoA K6,7R

    Plasmid

    #11751

    Depositor

    Jeff Wrana

    Article

    Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

    Insert

    RhoA (RHOA Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K6,7R

    Available Since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pGBW-m4046940

    Plasmid

    #149261

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    May 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pRK5 Flag RagB

    Plasmid

    #112755

    Purpose

    express Flag-RagB

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagB (RRAGB Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCellFree_G03 GATA1

    Plasmid

    #67068

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GATA1 (GATA1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pIS1 IMP-1 long UTR

    Plasmid

    #21640

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    IGF-II mRNA-binding protein 1 UTR (IGF2BP1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPRIME-TREX-GFP-FF3

    Plasmid

    #11667

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCellFree_G03 TNF

    Plasmid

    #67062

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TNF (TNF Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pMH-HT_ygiF-CHAD

    Plasmid

    #127793

    Purpose

    Expresses the CHAD domain of ygiF in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    ygiF-CHAD (ygiF Escherichia coli)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    N-terminal TTM domain of ygiF (residues 201-433)

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pGEX-NEMOL329P

    Plasmid

    #11967

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    NEMOL329P (IKBKG Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Leucine329 to Proline

    Available Since

    July 13, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pJKW1813

    Plasmid

    #174003

    Purpose

    Expresses PpCYP90G4 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

    Depositor

    Jing-Ke Weng

    Article

    Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

    Inserts

    PpCYP90G4
    ATR1 (ATR1 Mustard Weed)
    

    Expression

    Yeast

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGoldyTALEN-TUBA1B-TAL-2

    Plasmid

    #62854

    Purpose

    TALEN mediated gene editing at human TUBA1B locus

    Depositor

    Gaudenz Danuser

    Article

    Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

    Insert

    TALEN array
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    miniCAGG

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGoldyTALEN-TUBA1B-TAL-1

    Plasmid

    #62853

    Purpose

    TALEN mediated gene editing at human TUBA1B locus

    Depositor

    Gaudenz Danuser

    Article

    Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

    Insert

    TALEN array
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    miniCAGG

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mouse Cavin-1

    Plasmid

    #50441

    Purpose

    expresses mouse Cavin-1 in mammalian cells

    Depositor

    Paul Pilch

    Article

    Liu et al J Biol Chem. 2008 Feb 15;283(7):4314-22.

    Insert

    Cavin-1 (Cavin1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pBAD-BpBBS

    Plasmid

    #199467

    Purpose

    Arabinose-induced expression of His-tagged biliverdin-binding serpin of Boana punctata treefrog (BpBBS) in E. coli.

    Depositor

    Vladislav Verkhusha

    Article

    Manoilov et al J Mol Biol. 2022 Jan 30;434(2):167359. d

    Insert

    BpBBS
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. R10-3 /pQE-30

    Plasmid

    #79859

    Purpose

    The first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore.

    Depositor

    Konstantin Lukyanov

    Article

    Mishin et al Biochemistry. 2008 Apr 22;47(16):4666-73

    Insert

    R10-3
    

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Aequorea victoria green fluorescent protein (avGF…

    Promoter

    T5 promoter / lac operator

    Available Since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. B108-Otx2-IRES-Cre

    Plasmid

    #73993

    Purpose

    B108 enhancer of Blimp1 gene drives Otx2 (isoform b) and Cre expression.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    B108-Otx2 (isoform b)-IRES-Cre (Otx2 Mouse)
    

    Expression

    Mammalian

    Available Since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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