We narrowed to 11,284 results for: Vars;

1. pAAV-EFS-DjCas13d-P2A-mCherry-pA

   Plasmid

   #233027

   Purpose

   To Express HA tagged DjCas13d and mcherry from the mammalian EFS promoter. The DjCas13dx and mCherry are separated by a P2A site

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   DjCas13d/mCherry
   

   Use

   AAV

   Tags

   mCherry

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EFS-CasRx-P2A-mCherry-pA

   Plasmid

   #233025

   Purpose

   To Express HA tagged CasRX and mcherry from the mammalian EFS promoter. The CasRx and mCherry are separated by a P2A site

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   CasRx/mCherry
   

   Use

   AAV

   Tags

   mCherry

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EFS-hfCas13d-P2A-mCherry-pA

   Plasmid

   #233026

   Purpose

   To Express HA Tagged hfCas13d and mcherry from the mammalian EFS promoter. The hfCas13d and mCherry are separated by a P2A site

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   hfCas13d/mCherry
   

   Use

   AAV

   Tags

   mCherry

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-intron-CasRx-pA/EF1a-mCherry-WPRE-pa

   Plasmid

   #233032

   Purpose

   To Express HA tagged CasRX the CMV promoter. Also encodes a mCherry gene outside of the viral genome

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   CasRx and mCherry
   

   Use

   AAV

   Tags

   mCherry

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS puro -GFP-delta73-82 REX1-IRES-mCherry

   Plasmid

   #232246

   Purpose

   Mammalian expression of delta73-82 REX1-GFP-IRES-mCherry

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   delta73-82 REX1-GFP-IRES-mCherry (Zfp42 Mouse)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   delta73-82

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS puro -GFP-I77P I78P REX1-IRES-mCherry

   Plasmid

   #232245

   Purpose

   Mammalian expression of I77P I78P REX1-GFP-IRES-mCherry

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   I77P I78P REX1-GFP-IRES-mCherry (Zfp42 Mouse)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   I77P I78P

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. p35S-FHA-AtAGO4_gDNA

   Plasmid

   #216838

   Purpose

   N-term Flag-HA tagged Arabidopsis thaliana (Col-0) AGO4 gDNA under regulation of CaMV 35S promoter

   Depositor

   Arturo Marí-Ordóñez

   Article

   Dombey et al bioRxiv 2024.04.03.587901; doi: https://

   Insert

   ARGONAUTE4 (AGO4 Mustard Weed)
   

   Tags

   Flag-HA

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-eA3A-BE5-(NG)-PX458-EGFP

   Plasmid

   #229687

   Purpose

   CRISPR CBE plasmid (C to T edits): eA3A-BE5-NG engineered to include the sgRNA cloning backbone from PX458 and EGFP. Includes BbsI sites for sgRNA cloning downstream of U6 promoter.

   Depositor

   Deborah Marsh

   Article

   Dickson et al Hum Mol Genet. 2023 Jun 27:ddad105. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-parkin K161N

   Plasmid

   #227365

   Purpose

   For bacterial expression of parkin with a mutated pUbl/pUb binding site on RING0.

   Depositor

   Kalle Gehring

   Article

   Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

   Insert

   Parkin K161N (PRKN Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Chan…

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-parkin delta 101-109

    Plasmid

    #227364

    Purpose

    For bacterial expression of parkin with a mutated ACT.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin delta 101-109 (PRKN Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Resi…

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW57-CMV-MAAP6

    Plasmid

    #218245

    Purpose

    1) Ectopic expression of MAAP6 protein in mammalian cells. 2) Packaging MAAP6 sequence into lentivirus for stable expression in mammalian cells.

    Depositor

    David Schaffer

    Article

    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

    Insert

    Membrane-Associated Accessory Protein from Adeno-Associated Virus 6 driven by CMV promoter
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57-CMV-MAAP9

    Plasmid

    #218246

    Purpose

    1) Ectopic expression of MAAP9 protein in mammalian cells. 2) Packaging MAAP9 sequence into lentivirus for stable expression in mammalian cells.

    Depositor

    David Schaffer

    Article

    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

    Insert

    Membrane-Associated Accessory Protein from Adeno-Associated Virus 9 driven by CMV promoter
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Control-Control-LRG-GFP

    Plasmid

    #225873

    Purpose

    Two copies of guide RNA targeting a control sequence

    Depositor

    Janai Carr-Ascher

    Article

    Freeland et al Clin Cancer Res. 2024 Aug 23. doi: 10.11

    Insert

    Control
    

    Use

    CRISPR and Lentiviral

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBIOS1458

    Plasmid

    #171715

    Purpose

    expresses maize Glutamine Synthetase ZmGln1-3 cDNA under the control of the Cassava Vein Mosaic Virus promoter (CsVMV) (cassette 1) and the maize Rubisco small subunit promoter (ZmRbcS) (cassette 2)

    Depositor

    Christophe Sallaud

    Article

    Amiour et al Commun Biol. 2021 Sep 17;4(1):1095. doi:

    Insert

    ZmGln1-3
    

    Expression

    Plant

    Available Since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-ArgiNLS-miRFP670

    Plasmid

    #220600

    Purpose

    Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    miRFP670
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)

    Plasmid

    #220597

    Purpose

    Constitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mVenus-Q69M (ME)
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1a-ArgiNLS-mKate2

    Plasmid

    #220599

    Purpose

    Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mKate2
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-FLEX-ArgiNLS-EGFP

    Plasmid

    #220601

    Purpose

    Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCPH2A.X_K5Q-GS3

    Plasmid

    #204746

    Purpose

    Expression of Cas9 and human H2AX with the K5Q mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K5Q (H2AX Human)
    

    Expression

    Mammalian

    Mutation

    H2AX with the K5Q mutation

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCPH2A.X_K5R-GS3

    Plasmid

    #204747

    Purpose

    Expression of Cas9 and human H2AX with the K5R mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K5R (H2AX Human)
    

    Expression

    Mammalian

    Mutation

    H2AX with the K5R mutation

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCPH2A.X_K134L-GS3

    Plasmid

    #204750

    Purpose

    Expression of Cas9 and human H2AX with the K134L mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K134L (H2AX Human)
    

    Expression

    Mammalian

    Mutation

    H2AX with the K134L mutation

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCPH2A.X_K134M-GS3

    Plasmid

    #204751

    Purpose

    Expression of Cas9 and human H2AX with the K134M mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K134M (H2AX Human)
    

    Expression

    Mammalian

    Mutation

    H2AX with the K134M mutation

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCPH2A.X_K134A-GS3

    Plasmid

    #204752

    Purpose

    Expression of Cas9 and human H2AX with the K134A mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K134A (H2AX Human)
    

    Expression

    Mammalian

    Mutation

    H2AX with the K134A mutation

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p2701-AGER-tdTomato

    Plasmid

    #216470

    Purpose

    donor vector for targeting a tdTomato reporter to the human AGER locus at the endogenous ATG start site

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Insert

    tdTomato
    

    Use

    CRISPR

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p2707-pHAGE-Ef1aL-dsRed-UBC-tagBFP

    Plasmid

    #216476

    Purpose

    lentiviral dual expression of dsRed and tagBFP

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    dsRed
    tagBFP
    

    Use

    Lentiviral

    Promoter

    EF1aL and UBC

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. U6-sgRNA-hcr3

    Plasmid

    #193660

    Purpose

    Expression of tandem pre-sgRNA array hcr3 for LbCas12a

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    U6-CFTR-sgRNA-KLF4-sgRNA-TET1-sgRNA-tRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. U6-sgRNA-hcr5

    Plasmid

    #193661

    Purpose

    Expression of tandem pre-sgRNA array hcr5 for LbCas12a

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    U6-DNMT3B-sgRNA-KLF4-sgRNA-TET1-sgRNA-PRR5L-sgRNA-CFTR-sgRNA-tRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. U6-sgRNA-pcr4

    Plasmid

    #193662

    Purpose

    Expression of tandem pre-sgRNA array pcr4 for LbCas12a

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    U6-PUM1-sgRNA-GHR-sgRNA-HMGA2-sgRNA-PUM2-sgRNA-tRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-dCas12a-AID

    Plasmid

    #193647

    Purpose

    Expression of base editor dCas12a-AID in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-hAID, EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-dCas12a-BE

    Plasmid

    #193648

    Purpose

    Expression of base editor dCas12a-BE in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-rAPOBEC1, EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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