We narrowed to 26,826 results for: STI

1. pUC19/Pt-mChe

   Plasmid

   #183164

   Purpose

   Construct for transient expression of plastids-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   Pt-mCherry
   

   Tags

   57 aa of the Pisum sativum rubisco small subunit …

   Expression

   Plant

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE-Stat5a1*6

   Plasmid

   #130668

   Purpose

   Retroviral vector for the expression of constitutive Stat5a (puromycin resistance)

   Depositor

   Gerardo Ferbeyre

   Article

   Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

   Insert

   Stat5a (Stat5a Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   changed Histidine 298 to Arginine and Serine 710 …

   Promoter

   LTR

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-CMV-Flag-AAK1_IDG-K

   Plasmid

   #135280

   Purpose

   Gateway destination clone of AAK1 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

   Depositor

   Ben Major

   Insert

   Flag-AAK1 (AAK1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cdk2-DN-HA

   Plasmid

   #1885

   Depositor

   Sander van den Heuvel

   Article

   van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

   Insert

   Cdk2 (CDK2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D146N, dominant negative

   Available Since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cdk5-DN-HA

   Plasmid

   #1873

   Depositor

   Sander van den Heuvel

   Article

   van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

   Insert

   Cdk5 (CDK5 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D145N, dominant negative

   Available Since

   March 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS hShh (CT#401)

   Plasmid

   #13996

   Depositor

   Cliff Tabin

   Article

   Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

   Insert

   Shh (SHH Human)
   

   Expression

   Bacterial

   Available Since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. T7-RelA(KR)

   Plasmid

   #23251

   Depositor

   Warner Greene

   Article

   Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

   Insert

   NFkB RelA (RELA Human)
   

   Tags

   T7 gp10

   Expression

   Mammalian

   Mutation

   K218/221R, K310R

   Available Since

   March 15, 2010

   Availability

   Academic Institutions and Nonprofits only

8. 3XFLAG-NSarQ27G-VMAn-FKBP

   Plasmid

   #70229

   Purpose

   Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

   Depositor

   Perry Howard

   Article

   Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

   Insert

   alpha Sarcin (N-terminal portion)
   

   Tags

   3XFLAG, FK506- and rapamycin-binding protein (FKB…

   Expression

   Mammalian

   Mutation

   Changed Gln 27 to Gly

   Promoter

   CMV

   Available Since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

9. JC201: pMVP/Ad/5-35-DEST

   Plasmid

   #121847

   Purpose

   pMVP destination vector, empty 5/35 fiber-modified adenovirus vector backbone

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Adenoviral; Pmvp gateway destination vector

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLHDR03_mCherry_tCD19_unique guide

    Plasmid

    #124062

    Purpose

    Construction of 1st donor for scarless genome editing with unique guide

    Depositor

    Matthew Porteus

    Article

    Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

    Inserts

    mCherry
    tCD19
    

    Use

    Mouse Targeting; Human targeting

    Promoter

    Human UbC modified

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. ArcLight-S249

    Plasmid

    #36855

    Depositor

    Vincent Pieribone

    Article

    Jin et al Neuron. 2012 Sep 6;75(5):779-85.

    Insert

    fluorescent protein voltage sensor
    

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    simian CMV

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. GreenT-EC - pRSET

    Plasmid

    #193947

    Purpose

    Bacterial expression vector for a low-affinity calcium indicator

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC
    

    Tags

    His-Tag

    Expression

    Bacterial

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. EF1m-ELP

    Plasmid

    #170763

    Purpose

    EF1m laminin α1 peptide fused to I30-ELP (VPGIG)30

    Depositor

    Andrew MacKay

    Article

    Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

    Insert

    EF1m-ELP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. PKG I alpha WT

    Plasmid

    #16390

    Depositor

    Bernard Weinstein

    Article

    Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

    Insert

    PKG1 alpha (PRKG1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLXIN GFP

    Plasmid

    #132935

    Purpose

    MLV-based transfer vector expressing GFP and neomycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    GFP
    

    Use

    Lentiviral, RNAi, and Retroviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. p663-UBC-miniTurbo-V5-CDK14_IDG-K

    Plasmid

    #158172

    Purpose

    Gateway destination clone of CDK14 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK14 (CDK14 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-21(+)Tm9D8* TrpB

    Plasmid

    #207517

    Purpose

    Expression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serine

    Depositor

    Thomas Huber

    Article

    Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

    Insert

    Tryptophan synthase β-subunit
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC-94N

    Plasmid

    #53281

    Purpose

    Contains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and produces beta-carotene in E. coli when complemented with a gene encoding geranylgeranyl diphosphate synthase

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtY, crtI, crtB
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-NGFP-VMAn-FKBP

    Plasmid

    #70225

    Purpose

    Expresses N-terminal portion of GFP along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    eGFP
    

    Tags

    FK506- and rapamycin-binding protein (FKBP), Link…

    Expression

    Mammalian

    Mutation

    Changed Glu 125 to Ile, Deleted amino acids 129-2…

    Promoter

    CMV

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-FRB-VMAc-CGFP

    Plasmid

    #70226

    Purpose

    Expresses C-terminal portion of GFP along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    eGFP
    

    Tags

    FKBP-rapamycin binding (FRB) domain of the mammal…

    Expression

    Mammalian

    Mutation

    Changed Ile 129 to Cys, Deleted amino acids 1-128

    Promoter

    CMV

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pAC-PHYTipi

    Plasmid

    #53283

    Purpose

    Contains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces phytoene in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtE, idi, crtY, crtI, crtB
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. Map3k1

    Plasmid

    #29581

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

    Insert

    Map3k1 (MAP3K1 Human)
    

    Tags

    GST, HIS6, and TEV

    Expression

    Bacterial

    Mutation

    Constitutively Active. Contains catalytic domain …

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

23. p2CT-His-MBP-Lse_C2c2_WT

    Plasmid

    #83486

    Purpose

    Bacterial expression of WT L. seeligeri C2c2 CRISPR effector

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lse_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRN119

    Plasmid

    #176884

    Purpose

    Encodes STM and WUS2 for ectopic meristem induction in plants

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    CmYLCV:Luc+:HSP, Nos::WUS2:pinII, and 35:STM:35S
    

    Expression

    Plant

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Donor_eGP2AP_RC

    Plasmid

    #133784

    Purpose

    Library reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.

    Depositor

    Sriram Kosuri

    Article

    Davis et al Cell Syst. 2020 Jul 22;11(1):75-85.e7. d

    Type

    Empty backbone

    Tags

    eGFP-P2A-Puro

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-C1-TFIIB

    Plasmid

    #26675

    Depositor

    Sui Huang

    Article

    Chen et al Mol Biol Cell. 2002 Jan . 13(1):276-84.

    Insert

    TFIIB (GTF2B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pET28a-TRBP

    Plasmid

    #50351

    Purpose

    bacterial expression of TRBP

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

    Insert

    TRBP (TARBP2 Human)
    

    Tags

    His and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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28. pcDNA3.cytGAP

    Plasmid

    #78734

    Purpose

    Expression of Ca2+ sensor GAP in the cytosol

    Depositor

    Teresa Alonso

    Article

    Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

    Insert

    cytGAP
    

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRcCMV Cep215 (Nigg CW493)

    Plasmid

    #41152

    Depositor

    Erich Nigg

    Article

    Graser et al J Cell Sci. 2007 Dec 15;120(Pt 24):4321-

    Insert

    Cep215 (CDK5RAP2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p2CT-His-MBP-Lsh_C2c2_WT

    Plasmid

    #83487

    Purpose

    Bacterial expression of WT L. shahii C2c2 CRISPR effector

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lsh_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLenti CMV/TO DEST (775-1)

    Plasmid

    #17432

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA5FRT/Flag-LOC339287(MSL1)

    Plasmid

    #29762

    Depositor

    Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    MSL1 (MSL1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

33. NET47 pEGFP-N2 (593)

    Plasmid

    #61986

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET47 (TM7SF2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

34. 2xMyc-tdMCP-SNAP (tdMCP standard)

    Plasmid

    #226291

    Purpose

    Lentiviral plasmid expressing a tdMCP-protein standard. Used in LABEL-seq abundance assays.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Inserts

    2xMyc-tdMCP-SNAPtag
    EGFP-IRES-BlastR
    

    Use

    Lentiviral

    Tags

    2xMyc-tdMCP

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti X2 Puro DEST (w16-1)

    Plasmid

    #17296

    Purpose

    3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi; Destination vector

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p663-UBC-TurboID-V5-BRSK2_IDG-K

    Plasmid

    #135272

    Purpose

    Gateway destination clone of BRSK2 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TurboID-V5-BRSK2 (BRSK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pUC18/promSau/Lysostaphin

    Plasmid

    #125766

    Purpose

    Production of staphylolytic protein lysostaphin from a constitutive promoter

    Depositor

    Benedykt Wladyka

    Article

    Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

    Insert

    lysostaphin
    

    Expression

    Bacterial

    Mutation

    sequence corresponding to the mature form protein

    Promoter

    pemIK constitutive promoter

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV5B-TGFbeta receptor I K232R

    Plasmid

    #11763

    Depositor

    Jeff Wrana

    Article

    Wieser et al EMBO J. 1995 May 15. 14(10):2199-208.

    Insert

    TGFbeta receptor I (TGFBR1 Human)
    

    Tags

    6xHIS

    Expression

    Mammalian

    Mutation

    K232R: kinase dead

    Promoter

    CMV

    Available Since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. p667-UBC-CDK12-V5_IDG-K

    Plasmid

    #135245

    Purpose

    Gateway destination clone of CDK12 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    CDK12-V5 (CDK12 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pC0044-EF1a-RanCas13b-NES-mapk

    Plasmid

    #103855

    Purpose

    Expresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    RanCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and mapk NES

    Expression

    Mammalian

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge