We narrowed to 10,636 results for: t7

1. pWUKI Cas1 Cas2 E9Q

   Plasmid

   #80091

   Purpose

   Encodes Cas1 and Cas2 E9Q

   Depositor

   George Church

   Article

   Shipman et al Science. 2016 Jun 9. pii: aaf1175.

   Inserts

   Cas1 (cas1 )
   Cas2 (cas2 )
   CRISPR Array (bp1542-2899)
   

   Expression

   Bacterial

   Mutation

   E9Q

   Promoter

   T7

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWUKI Cas1 V134L Cas2

   Plasmid

   #80092

   Purpose

   Encodes Cas1 V134L and Cas2

   Depositor

   George Church

   Article

   Shipman et al Science. 2016 Jun 9. pii: aaf1175.

   Inserts

   Cas1 (cas1 )
   Cas2 (cas2 )
   CRISPR Array
   

   Expression

   Bacterial

   Mutation

   V134L

   Promoter

   T7

   Availability

   Academic Institutions and Nonprofits only

   

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3. enz.043-SETD7-Y305F

   Plasmid

   #154045

   Purpose

   Expresses SETD7 (Y305F) in E. coli

   Depositor

   Christopher Voigt

   Article

   Wallace et al Advanced Functional Materials 2020

   Insert

   Histone-lysine N-methyltransferase SETD7 (SETD7 Human)
   

   Tags

   His, MBP

   Expression

   Bacterial

   Mutation

   changed tyrosine 305 to phenylalanine

   Promoter

   T7

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNatB (pACYCduet-naa20-naa25)

   Plasmid

   #53613

   Purpose

   Allows expression of the fission yeast NatB complex - chloramphenicol marker

   Depositor

   Dan Mulvihill

   Article

   Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

   Inserts

   naa20 (naa20 Fission Yeast)
   naa25 (naa25 Fission Yeast)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-6xHis-GFP(-30)

   Plasmid

   #199167

   Purpose

   used to express a supercharged GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(-30)
   

   Tags

   MGHHHHHHGGA

   Mutation

   T19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …

   Promoter

   T7

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFGET19_Ulp1

   Plasmid

   #64697

   Purpose

   H6-Ulp1 from pHYRS52 into pET-28b(+)

   Depositor

   Hideo Iwai

   Article

   Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

   Insert

   ULP1 (ULP1 Budding Yeast, Saccharomyces cerevisiae)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Ulp1(403-621)

   Promoter

   T7

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. S96 ELP

   Plasmid

   #68393

   Purpose

   Elastin-like polypeptide (VPGxG) with 96 repeats of Serine guest residue

   Depositor

   Andrew MacKay

   Article

   Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

   Insert

   S96 ELP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. V96 ELP

   Plasmid

   #68392

   Purpose

   Elastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residue

   Depositor

   Andrew MacKay

   Article

   Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

   Insert

   V96 ELP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b-AavLEA1

   Plasmid

   #53093

   Purpose

   AavLEA1 expression (in BL21 DE3 E. coli strain)

   Depositor

   Claude Férec

   Article

   Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

   Insert

   AavLEA1
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACYC-Skp-GroEL/ES

    Plasmid

    #83922

    Purpose

    Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Skp
    Gro EL/ES
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET_mNG2(1-10)_32aalinker_mNG2(11)

    Plasmid

    #82611

    Purpose

    Bacterial expression vector for split mNeonGreen2 screening

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
    

    Expression

    Bacterial

    Mutation

    split before mutations

    Promoter

    T7

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACYC-Skp-TF

    Plasmid

    #83921

    Purpose

    Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Skp
    Trigger Factor
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. V48 ELP

    Plasmid

    #68395

    Purpose

    Elastin-like polypeptide (VPGxG) with 48 repeats of valine guest residue

    Depositor

    Andrew MacKay

    Article

    Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

    Insert

    V48 ELP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET30b-7+-SrtA

    Plasmid

    #105602

    Purpose

    expressed 7+ sortase A variant in E.coli

    Depositor

    Stephanie Dougan

    Article

    Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

    Insert

    S.aureus Sortase A, 7+ mutant
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIA1414

    Plasmid

    #172402

    Purpose

    Expression of SARS-COV-2 Nsp9 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp9 (ORF1ab Synthetic)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21b FAST-PETase

    Plasmid

    #225760

    Purpose

    Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag

    Depositor

    Jason Boock

    Article

    Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

    Insert

    FAST-PETase
    

    Tags

    6xHis Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSF-1b-S2

    Plasmid

    #128590

    Purpose

    Expresses tagless S2 ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S2 (rpsB E.coli)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPSU10-30-50-100

    Plasmid

    #173838

    Purpose

    Coexpresses 10, 30, 50 and 100 kD Penn State ladder proteins

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPAB
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7-CalfluxVTN

    Plasmid

    #83926

    Purpose

    Bioluminescent Reporter of Intracellular Calcium

    Depositor

    Carl Johnson

    Article

    Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

    Insert

    CalfluxVTN
    

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTYB1-eIF4A

    Plasmid

    #37225

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Insert

    eIF4A
    

    Tags

    intein

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only